PACMotif C-terminal to PAS motifs (likely to contribute to PAS structural domain)
|SMART accession number:||SM00086|
|Description:||PAC motif occurs C-terminal to a subset of all known PAS motifs. It is proposed to contribute to the PAS domain fold.|
|Interpro abstract (IPR001610):|
Click on the following links for more information.
- Evolution (species in which this domain is found)
Taxonomic distribution of proteins containing PAC domain.
This tree includes only several representative species. The complete taxonomic breakdown of all proteins with PAC domain is also avaliable.
Click on the protein counts, or double click on taxonomic names to display all proteins containing PAC domain in the selected taxonomic class.
- Literature (relevant references for this domain)
Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.
- Pellequer JL, Wager-Smith KA, Kay SA, Getzoff ED
- Photoactive yellow protein: a structural prototype for the three-dimensional fold of the PAS domain superfamily.
- Proc Natl Acad Sci U S A. 1998; 95: 5884-90
- Display abstract
PAS domains are found in diverse proteins throughout all three kingdoms of life, where they apparently function in sensing and signal transduction. Although a wealth of useful sequence and functional information has become recently available, these data have not been integrated into a three-dimensional (3D) framework. The very early evolutionary development and diverse functions of PAS domains have made sequence analysis and modeling of this protein superfamily challenging. Limited sequence similarities between the approximately 50-residue PAS repeats and one region of the bacterial blue-light photosensor photoactive yellow protein (PYP), for which ground-state and light-activated crystallographic structures have been determined to high resolution, originally were identified in sequence searches using consensus sequence probes from PAS-containing proteins. Here, we found that by changing a few residues particular to PYP function, the modified PYP sequence probe also could select PAS protein sequences. By mapping a typical approximately 150-residue PAS domain sequence onto the entire crystallographic structure of PYP, we show that the PAS sequence similarities and differences are consistent with a shared 3D fold (the PAS/PYP module) with obvious potential for a ligand-binding cavity. Thus, PYP appears to prototypically exhibit all the major structural and functional features characteristic of the PAS domain superfamily: the shared PAS/PYP modular domain fold of approximately 125-150 residues, a sensor function often linked to ligand or cofactor (chromophore) binding, and signal transduction capability governed by heterodimeric assembly (to the downstream partner of PYP). This 3D PAS/PYP module provides a structural model to guide experimental testing of hypotheses regarding ligand-binding, dimerization, and signal transduction.
- Rutila JE, Suri V, Le M, So WV, Rosbash M, Hall JC
- CYCLE is a second bHLH-PAS clock protein essential for circadian rhythmicity and transcription of Drosophila period and timeless.
- Cell. 1998; 93: 805-14
- Display abstract
We report the identification, characterization, and cloning of another novel Drosophila clock gene, cycle (cyc). Homozygous cyc flies are completely arrhythmic. Heterozygous cyc/+ flies are rhythmic but have altered periods, indicating that the cyc locus has a dosage effect on period. The molecular circadian phenotype of homozygous cyc flies is like homozygous Clk flies presented in the accompanying paper: mutant flies have little or no transcription of the per and tim genes. Cloning of the gene indicates that it also encodes a bHLH-PAS transcription factor and is a Drosophila homolog of the human protein BMAL1. cyc is a nonsense mutation, consistent with its strong loss-of-function phenotype. We propose that the CYC:CLK heterodimer binds to per and tim E boxes and makes a major contribution to the circadian transcription of Drosophila clock genes.
- Ponting CP, Aravind L
- PAS: a multifunctional domain family comes to light.
- Curr Biol. 1997; 7: 6747-6747
- Zelzer E, Wappner P, Shilo BZ
- The PAS domain confers target gene specificity of Drosophila bHLH/PAS proteins.
- Genes Dev. 1997; 11: 2079-89
- Display abstract
Trachealess (Trh) and Single-minded (Sim) are highly similar Drosophila bHLH/PAS transcription factors. They activate nonoverlapping target genes and induce diverse cell fates. A single Drosophila gene encoding a bHLH/PAS protein homologous to the vertebrate ARNT protein was isolated and may serve as a partner for both Trh and Sim. We show that Trh and Sim complexes recognize similar DNA-binding sites in the embryo. To examine the basis for their distinct target gene specificity, the activity of Trh-Sim chimeric proteins was monitored in embryos. Replacement of the Trh PAS domain by the analogous region of Sim was sufficient to convert it into a functional Sim protein. The PAS domain thus mediates all the features conferring specificity and the distinct recognition of target genes. The normal expression pattern of additional proteins essential for the activity of the Trh or Sim complexes can be inferred from the induction pattern of target genes and binding-site reporters, triggered by ubiquitous expression of Trh or Sim. We postulate that the capacity of bHLH/PAS heterodimers to associate, through the PAS domain, with additional distinct proteins that bind target-gene DNA, is essential to confer specificity.
- Zhulin IB, Taylor BL, Dixon R
- PAS domain S-boxes in Archaea, Bacteria and sensors for oxygen and redox.
- Trends Biochem Sci. 1997; 22: 331-3
- Borgstahl GE, Williams DR, Getzoff ED
- 1.4 A structure of photoactive yellow protein, a cytosolic photoreceptor: unusual fold, active site, and chromophore.
- Biochemistry. 1995; 34: 6278-87
- Display abstract
A photosensing protein directs light energy captured by its chromophore into a photocycle. The protein's structure must accommodate the photocycle and promote the resulting chemical or conformational changes that lead to signal transduction. The 1.4 A crystallographic structure of photoactive yellow protein, determined by multiple isomorphous replacement methods, provides the first view at atomic resolution of a protein with a photocycle. The alpha/beta fold, which differs from the original chain tracing, shows striking similarity to distinct parts of the signal transduction proteins profilin and the SH2 domain. In the dark state structure of photoactive yellow protein, the novel 4-hydroxycinnamyl chromophore, covalently attached to Cys69, is buried within the major hydrophobic core of the protein and is tethered at both ends by hydrogen bonds. In the active site, the yellow anionic form of the chromophore is stabilized by hydrogen bonds from the side chains of Tyr42 and buried Glu46 to the phenolic oxygen atom and by electrostatic complementarity with the positively charged guanidinium group of Arg52. Thr50 further interlocks Tyr42, Glu46, and Arg52 through a network of active site hydrogen bonds. Arg52, located in a concavity of the protein surface adjacent to the dominant patch of negative electrostatic potential, shields the chromophore from solvent and is positioned to form a gateway for the phototactic signal. Overall, the high-resolution structure of photoactive yellow protein supports a mechanism whereby electrostatic interactions create an active site poised for photon-induced rearrangements and efficient protein-mediated signal transduction.
- Metabolism (metabolic pathways involving proteins which contain this domain)
Click the image to view the interactive version of the map in iPath
% proteins involved KEGG pathway ID Description 38.51 map02030 Bacterial chemotaxis - General 31.28 map02020 Two-component system - General 9.57 map04710 Circadian rhythm 7.66 map05211 Renal cell carcinoma 2.13 map04150 mTOR signaling pathway 1.70 map03010 Ribosome 1.28 map00230 Purine metabolism 0.64 map00632 Benzoate degradation via CoA ligation 0.64 map00720 Reductive carboxylate cycle (CO2 fixation) 0.64 map04914 Progesterone-mediated oocyte maturation 0.43 map00650 Butanoate metabolism 0.43 map00562 Inositol phosphate metabolism 0.43 map00020 Citrate cycle (TCA cycle) 0.43 map04540 Gap junction 0.43 map04730 Long-term depression 0.21 map00350 Tyrosine metabolism 0.21 map00260 Glycine, serine and threonine metabolism 0.21 map00970 Aminoacyl-tRNA biosynthesis 0.21 map00910 Nitrogen metabolism 0.21 map00620 Pyruvate metabolism 0.21 map00400 Phenylalanine, tyrosine and tryptophan biosynthesis 0.21 map02040 Flagellar assembly 0.21 map00340 Histidine metabolism 0.21 map04810 Regulation of actin cytoskeleton 0.21 map04020 Calcium signaling pathway 0.21 map00633 Trinitrotoluene degradation 0.21 map00190 Oxidative phosphorylation 0.21 map00860 Porphyrin and chlorophyll metabolism 0.21 map04510 Focal adhesion 0.21 map05060 Prion disease 0.21 map00640 Propanoate metabolism 0.21 map00540 Lipopolysaccharide biosynthesis 0.21 map03030 DNA replication
This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with PAC domain which could be assigned to a KEGG orthologous group, and not all proteins containing PAC domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.
- Structure (3D structures containing this domain)
3D Structures of PAC domains in PDB
PDB code Main view Title 1byw STRUCTURE OF THE N-TERMINAL DOMAIN OF THE HUMAN-ERG POTASSIUM CHANNEL 1dp6 OXYGEN-BINDING COMPLEX OF FIXL HEME DOMAIN 1dp8 CRYSTAL STRUCTURE OF THE NITRIC OXIDE BOUND FIXL HEME DOMAIN 1dp9 CRYSTAL STRUCTURE OF IMIDAZOLE-BOUND FIXL HEME DOMAIN 1drm CRYSTAL STRUCTURE OF THE LIGAND FREE BJFIXL HEME DOMAIN 1dwy Bovine prion protein fragment 121-230 1dwz Bovine prion protein fragment 121-230 1dx0 BOVINE PRION PROTEIN RESIDUES 23-230 1dx1 BOVINE PRION PROTEIN RESIDUES 23-230 1g28 STRUCTURE OF A FLAVIN-BINDING DOMAIN, LOV2, FROM THE CHIMERIC PHYTOCHROME/PHOTOTROPIN PHOTORECEPTOR PHY3 1jnu Photoexcited structure of the plant photoreceptor domain, phy3 LOV2 1lsv Crystal structure of the CO-bound BjFixL heme domain 1lsw Crystal structure of the ferrous BjFixL heme domain 1lsx Crystal structure of the methylimidazole-bound BjFixL heme domain 1lt0 Crystal structure of the CN-bound BjFixL heme domain 1n9l Crystal structure of the Phot-LOV1 domain from Chlamydomonas reinhardtii in the dark state. 1n9n Crystal structure of the Phot-LOV1 domain from Chlamydomonas reinhardtii in illuminated state. Data set of a single crystal. 1n9o Crystal structure of the Phot-LOV1 domain from Chlamydomonas reinhardtii in illuminated state. Composite data set. 1p97 NMR structure of the C-terminal PAS domain of HIF2a 1s66 Crystal structure of heme domain of direct oxygen sensor from E. coli 1s67 Crystal structure of heme domain of direct oxygen sensor from E. coli 1v9y Crystal Structure of the heme PAS sensor domain of Ec DOS (ferric form) 1v9z Crystal Structure of the heme PAS sensor domain of Ec DOS (Ferrous Form) 1vb6 Crystal Structure of the heme PAS sensor domain of Ec DOS (oxygen-bound form) 1wa9 Crystal Structure of the PAS repeat region of the Drosophila clock protein PERIOD 1x0o human ARNT C-terminal PAS domain 1xj2 CO-bound structure of bjFixLH 1xj3 bjFixLH in unliganded ferrous form 1xj4 CO-bound structure of BjFixLH 1xj6 Structure of bjFixLH in the unliganded ferrous form 1y28 Crystal structure of the R220A metBJFIXL HEME domain 2a24 HADDOCK Structure of HIF-2a/ARNT PAS-B Heterodimer 2b02 Crystal Structure of ARNT PAS-B Domain 2cmn A Proximal Arginine Residue in the Switching Mechanism of the FixL Oxygen Sensor 2gj3 Crystal structure of the FAD-containing PAS domain of the protein NifL from Azotobacter vinelandii. 2hv1 HADDOCK structure of ARNT PAS-B Homodimer 2k7s Human ARNT C-Terminal PAS Domain, 3 Residue IB slip 2kdk Structure of human circadian clock protein BMAL2 C-terminal PAS domain 2l0w Solution NMR structure of the N-terminal PAS domain of HERG potassium channel 2l1m Solution structure of the eag domain of the hERG (Kv11.1) K+ channel 2l4r NMR solution structure of the N-terminal PAS domain of hERG 2owh Structure of an early-microsecond photolyzed state of CO-bjFixLH 2owj Structure of an early-microsecond photolyzed state of CO-bjFixLH, dark state 2pd7 2.0 Angstrom Crystal Structure of the Fungal Blue-Light Photoreceptor Vivid 2pd8 1.8 Angstrom Crystal Structure of the Cys71Ser mutant of Vivid 2pdr 1.7 Angstrom Crystal Structure of the Photo-excited Blue-light Photoreceptor Vivid 2pdt 2.3 Angstrom Structure of Phosphodiesterase treated Vivid 2pr5 Structural Basis for Light-dependent Signaling in the Dimeric LOV Photosensor YtvA (Dark Structure) 2pr6 Structural Basis for Light-dependent Signaling in the Dimeric LOV Photosensor YtvA (Light Structure) 2v0u n- and c-terminal helices of oat lov2 (404-546) are involved in light-induced signal transduction (cryo dark structure of lov2 (404-546)) 2v0w N- and C-terminal helices of oat LOV2 (404-546) are involved in light- induced signal transduction (cryo-trapped light structure of LOV2 ( 404-546)) 2v1a N- and C-terminal helices of oat LOV2 (404-546) are involved in light-induced signal transduction (room temperature (293K) dark structure of LOV2 (404-546)) 2v1b N- and C-terminal helices of oat LOV2 (404-546) are involved in light-induced signal transduction (room temperature (293K) light structure of LOV2 (404-546)) 2vlg KinA PAS-A domain, homodimer 2vv6 BJFIXLH IN FERRIC FORM 2vv7 BJFIXLH IN UNLIGANDED FERROUS FORM 2vv8 Molecular mechanism of signal transduction in bjFixL 2wkp Structure of a photoactivatable Rac1 containing Lov2 Wildtype 2wkq Structure of a photoactivatable Rac1 containing the Lov2 C450A Mutant 2wkr Structure of a photoactivatable Rac1 containing the Lov2 C450M Mutant 2z6c Crystal structure of LOV1 domain of phototropin1 from Arabidopsis thaliana 2z6d Crystal structure of LOV1 domain of phototropin2 from Arabidopsis thaliana 3d72 1.65 Angstrom crystal structure of the Cys71Val variant in the fungal photoreceptor VVD 3ewk Structure of the redox sensor domain of Methylococcus capsulatus (Bath) MmoS 3f1n Crystal structure of a high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains, with internally bound ethylene glycol. 3f1o Crystal structure of the high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains, with an internally-bound artificial ligand 3f1p Crystal structure of a high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains 3gdi Mammalian Clock Protein mPER2 - Crystal Struture of a PAS Domain Fragment 3gec Crystal structure of a tandem PAS domain fragment of Drosophila PERIOD 3h7w Crystal structure of the high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains with the artificial ligand THS017 3h82 Crystal structure of the high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains with the artificial ligand THS020 3h9w Crystal Structure of the N-terminal domain of Diguanylate cyclase with PAS/PAC sensor (Maqu_2914) from Marinobacter aquaeolei, Northeast Structural Genomics Consortium Target MqR66C 3hji 1.8 Angstrom Crystal Structure of the I74V:I85V Variant of Vivid (VVD). 3hjk 2.0 Angstrom Structure of the Ile74Val Variant of Vivid (VVD). 3is2 2.3 Angstrom Crystal Structure of a Cys71 Sulfenic Acid form of Vivid 3k3c The N-terminal PAS domain crystal structure of Rv1364c from Mycobacterium tuberculosis at 1.62 3k3d The N-terminal PAS domain crystal structure of RV1364C from Mycobacterium Tuberculosis at 2.3 angstrom 3kx0 Crystal Structure of the PAS domain of Rv1364c 3lyx Crystal structure of the PAS domain of the protein CPS_1291 from Colwellia psychrerythraea. Northeast Structural Genomics Consortium target id CsR222B 3mr0 Crystal Structure of Sensory Box Histidine Kinase/Response Regulator from Burkholderia thailandensis E264 3nja The crystal structure of the PAS domain of a GGDEF family protein from Chromobacterium violaceum ATCC 12472. 3p7n Crystal structure of light activated transcription factor El222 from Erythrobacter litoralis 3rh8 Crystal Structure of the Light-state Dimer of Fungal Blue-Light Photoreceptor Vivid 3rty Structure of an Enclosed Dimer Formed by The Drosophila Period Protein 3sw1 Structure of a full-length bacterial LOV protein 3t50 X-ray structure of the LOV domain from the LOV-HK sensory protein from Brucella abortus (dark state). 3ue6 The dark structure of the blue-light photoreceptor Aureochrome1 LOV 3ulf The light state structure of the blue-light photoreceptor Aureochrome1 LOV 4dj2 Unwinding the Differences of the Mammalian PERIOD Clock Proteins from Crystal Structure to Cellular Function 4dj3 Unwinding the Differences of the Mammalian PERIOD Clock Proteins from Crystal Structure to Cellular Function 4eep Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2 4eer Crystal structure of LOV2 domain of Arabidopsis thaliana phototropin 2 C426A mutant 4ees Crystal structure of iLOV 4eet Crystal structure of iLOV 4eeu Crystal structure of phiLOV2.1 4eq1 Crystal Structure of the ARNT PAS-B homodimer 4f3l Crystal Structure of the Heterodimeric CLOCK:BMAL1 Transcriptional Activator Complex 4gcz Structure of a blue-light photoreceptor 4ghi Crystal structure of the high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains in complex with a benzoxadiazole antagonist 4gs9 Crystal structure of the high affinity heterodimer of HIF2 alpha and ARNT C-terminal PAS domains in complex with an inactive benzoxadiazole antagonist 4gw9 Structure of a bacteriophytochrome and light-stimulated protomer swapping with a gene repressor 4h6j Identification of Cys 255 in HIF-1 as a novel site for development of covalent inhibitors of HIF-1 /ARNT PasB domain protein-protein interaction. 4hhd 2.75 Angstrom resolution crystal structure of the A. thaliana LOV2 domain with an extended N-terminal A' helix (cryo dark structure) 4hoi Crystal structure of PAS domain from the mouse EAG1 potassium channel 4hp4 Crystal structure of PAS domain from the fruit-fly ELK potassium channel 4hp9 Crystal structure of the N-terminal truncated PAS domain from the hERG potassium channel 4hqa Crystal structure of PAS domain from the human ERG (hERG) potassium channel 4i5s Structure and function of sensor histidine kinase 4llo Structure of the eag domain-CNBHD complex of the mouse EAG1 channel
- Links (links to other resources describing this domain)