SMART: LamG domain annotation

LamG Laminin G domain

SMART accession number:	SM00282
Description:
Interpro abstract (IPR001791):	Laminins are large heterotrimeric glycoproteins involved in basement membrane function (PUBMED:15037599). The laminin globular (G) domain can be found in one to several copies in various laminin family members, including a large number of extracellular proteins. The C-terminus of the laminin alpha chain contains a tandem repeat of five laminin G domains, which are critical for heparin-binding and cell attachment activity (PUBMED:10747011). Laminin alpha4 is distributed in a variety of tissues including peripheral nerves, dorsal root ganglion, skeletal muscle and capillaries; in the neuromuscular junction, it is required for synaptic specialisation (PUBMED:15823034). The structure of the laminin-G domain has been predicted to resemble that of pentraxin (PUBMED:9480764). Laminin G domains can vary in their function, and a variety of binding functions have been ascribed to different LamG modules. For example, the laminin alpha1 and alpha2 chains each have five C-teminal laminin G domains, where only domains LG4 and LG5 contain binding sites for heparin, sulphatides and the cell surface receptor dystroglycan (PUBMED:10747011). Laminin G-containing proteins appear to have a wide variety of roles in cell adhesion, signalling, migration, assembly and differentiation. Proteins with laminin-G domains include: Laminin. Merosin. Agrin. Neurexins. Vitamin K dependent protein S. Sex steroid binding protein SBP/SHBG. Drosophila proteins Slit, Crumbs, Fat. several proteoglycan precursors.
Family alignment:	View or

There are 4181 LamG domains in 1857 proteins in SMART's nrdb database.

Click on the following links for more information.

Evolution (species in which this domain is found)
Click on to expand nodes. To display all proteins with a LamG domain in a specific node, click on it.

This tree shows only several representative species. The complete taxonomic breakdown of all proteins with LamG domain is also avaliable.

Useful shortcuts: Expand all nodes or Collapse tree
Go to specific node: Anopheles gambiae, Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens, Mus musculus, Rattus norvegicus, Takifugu rubripes
Literature (relevant references for this domain)
Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.
- Beckmann G, Hanke J, Bork P, Reich JG
- Merging extracellular domains: fold prediction for laminin G-like and amino-terminal thrombospondin-like modules based on homology to pentraxins.
- J Mol Biol. 1998; 275: 725-30
- Display abstract
- Using a new method for construction and database searches of sequence consensus strings, we have identified a new superfamily of protein modules comprising laminin G, thrombospondin N and the pentraxin families. The conserved patterns correspond mainly to hydrophobic core residues located in central beta strands of the known three-dimensional structures of two pentraxins, the human C-reactive protein and the serum amyloid P-component. Thus, we predict a similar jellyroll fold for all members of this superfamily. In addition, the conservation of two exposed aspartate residues in the majority of superfamily members suggests hitherto unrecognised functional sites.
Disease (disease genes where sequence variants are found in this domain)
SwissProt sequences and OMIM curated human diseases associated with missense mutations within the LamG domain.

Protein Disease

Laminin subunit alpha-2 precursor (SRS)(SMART) OMIM:156225: Muscular dystrophy, congenital merosin-deficient
Metabolism (metabolic pathways involving proteins which contain this domain)

Structure (3D structures containing this domain)

Protein	Disease
Laminin subunit alpha-2 precursor (SRS)(SMART)	OMIM:156225: Muscular dystrophy, congenital merosin-deficient

3D Structures of LamG domains in PDB

PDB code	Main view	Title
1c4r		The structure of the ligand-binding domain of neurexin 1beta: regulation of lns domain function by alternative splicing
1d2s		Crystal structure of the n-terminal laminin g-like domain of shbg in complex with dihydrotestosterone
1dyk		Laminin alpha 2 chain lg4-5 domain pair
1f5f		Crystal structure of the n-terminal g-domain of shbg in complex with zinc
1h30		C-terminal lg domain pair of human gas6
1kdk		The structure of the n-terminal lg domain of shbg in crystals soaked with edta
1kdm		The crystal structure of the human sex hormone-binding globulin (tetragonal crystal form)
1lhn		Crystal structure of the n-terminal lg-domain of shbg in complex with 5alpha-androstane-3beta,17alpha-diol
1lho		Crystal structure of the n-terminal lg-domain of shbg in complex with 5alpha-androstane-3beta,17beta-diol
1lhu		Crystal structure of the n-terminal lg-domain of shbg in complex with estradiol
1lhv		Crystal structure of the n-terminal lg-domain of shbg in complex with norgestrel
1lhw		Crystal structure of the n-terminal lg-domain of shbg in complex with 2-methoxyestradiol
1okq		Laminin alpha 2 chain lg4-5 domain pair, ca1 site mutant
1pz7		Modulation of agrin function by alternative splicing and ca2+ binding
1pz8		Modulation of agrin function by alternative splicing and ca2+ binding
1pz9		Modulation of agrin function by alternative splicing and ca2+ binding
1q56		Nmr structure of the b0 isoform of the agrin g3 domain in its ca2+ bound state
1qu0		Crystal structure of the fifth laminin g-like module of the mouse laminin alpha2 chain
1sli		Leech intramolecular trans-sialidase complexed with dana
1sll		Sialidase l from leech macrobdella decora
2c5d		Structure of a minimal gas6-axl complex
2h0b		Crystal structure of the second lns/lg domain from neurexin alpha
2jd4		Mouse laminin alpha1 chain, domains lg4-5
2r16		Crystal structure of bovine neurexin 1 alpha lns/lg domain (with no splice insert)
2r1b		Crystal structure of rat neurexin 1beta with a splice insert at ss#4
2r1d		Crystal structure of rat neurexin 1beta in the ca2+ containing form
2sli		Leech intramolecular trans-sialidase complexed with 2,7- anhydro-neu5ac, the reaction product
2wjs
2wqz
3b3q		Crystal structure of a synaptic adhesion complex
3biw		Crystal structure of the neuroligin-1/neurexin-1beta synaptic adhesion complex
3bod		Structure of mouse beta-neurexin 1
3bop		Structure of mouse beta-neurexin 2d4
3sli		Leech intramolecular trans-sialidase complexed with 2,7- anhydro-neu5ac prepared by soaking with 3'-sialyllactose
4sli		Leech intramolecular trans-sialidase complexed with 2- propenyl-neu5ac, an inactive substrate analogue

Links (links to other resources describing this domain)
PFAM laminin_G
INTERPRO IPR001791

PFAM	laminin_G
INTERPRO	IPR001791