Schultz et al. (1998) Proc. Natl. Acad. Sci. USA 95, 5857-5864
Letunic et al. (2008) Nucleic Acids Res , doi:10.1093/nar/gkn808

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PWI PWI, domain in splicing factors

There are 113 PWI domains in 113 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Click on to expand nodes. To display all proteins with a PWI domain in a specific node, click on it.

  This tree shows only several representative species. The complete taxonomic breakdown of all proteins with PWI domain is also avaliable.

  Useful shortcuts: Expand all nodes or Collapse tree
  Go to specific node: Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae, Takifugu rubripes

• Literature (relevant references for this domain)

• Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.

  • Szymczyna BR et al.
  • Structure and function of the PWI motif: a novel nucleic acid-binding domain that facilitates pre-mRNA processing.
  • Genes Dev. 2003; 17: 461-75
  • Display abstract

  • The PWI motif is a highly conserved domain of unknown function in the SRm160 splicing and 3'-end cleavage-stimulatory factor, as well as in several other known or putative pre-mRNA processing components. We show here that the PWI motif is a new type of RNA/DNA-binding domain that has an equal preference for single- and double-stranded nucleic acids. Deletion of the motif prevents SRm160 from binding RNA and stimulating 3'-end cleavage, and its substitution with a heterologous RNA-binding domain restores these functions. The NMR solution structure of the SRm160-PWI motif reveals a novel, four-helix bundle and represents the first example of an alpha-helical fold that can bind single-stranded (ss)RNA. Structure-guided mutagenesis indicates that the same surface is involved in RNA and DNA binding and requires the cooperative action of a highly conserved, adjacent basic region. Thus, the PWI motif is a novel type of nucleic acid-binding domain that likely has multiple important functions in pre-mRNA processing, including SRm160-dependent stimulation of 3'-end formation.

• Structure (3D structures containing this domain)

• 3D Structures of PWI domains in PDB

  PDB code	Main view	Title
  1mp1		Solution structure of the pwi motif from srm160
  1x4q		Solution structure of pwi domain in u4/u6 small nuclear ribonucleoprotein prp3(hprp3)

• Links (links to other resources describing this domain)

• INTERPRO	IPR002483