SMART: AAI domain annotation

AAI Plant lipid transfer protein / seed storage protein / trypsin-alpha amylase inhibitor domain family

SMART accession number:	SM00499
Description:
Interpro abstract (IPR016140):	This entry represents a structural domain consisting of 4-helices with a folded leaf topology, and forming a right-handed superhelix. This domain occurs in several proteins, including: Plant lipid-transfer proteins, such as the non-specific lipid-transfer proteins ns-LTP1 and ns-LTP2 [(PUBMED:10491104), (PUBMED:12011089)]. Proteinase/alpha-amylase inhibitors, such as trypsin/alpha-amylase inhibitor RBI from Eleusine coracana (Indian finger millet) [(PUBMED:9687373)] and Hageman factor/amylase inhibitor from Zea mays (Maize) [(PUBMED:9799488)]. Seed storage proteins, such as Napin from Brassica napus (Rape) [(PUBMED:8961930)] and 2S albumin from Ricinus communis (Castor bean) [(PUBMED:14636051)].
Family alignment:	View or

There are 2196 AAI domains in 2156 proteins in SMART's nrdb database.

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Evolution (species in which this domain is found)
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This tree shows only several representative species. The complete taxonomic breakdown of all proteins with AAI domain is also avaliable.

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Go to specific node: Arabidopsis thaliana, Caenorhabditis elegans
Literature (relevant references for this domain)
Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.
- Wirtz KW
- Phospholipid transfer proteins.
- Annu Rev Biochem. 1991; 60: 73-99
- Gautier MF, Alary R, Joudrier P
- Cloning and characterization of a cDNA encoding the wheat (Triticum durum Desf.) CM16 protein.
- Plant Mol Biol. 1990; 14: 313-22
- Display abstract
- A Triticum durum cDNA library prepared from developing endosperm (22 days after flowering (DAF] was screened using synthetic oligonucleotide probes covering part of the CM3 and CM16 N-terminal protein sequences. A full-length cDNA clone (pTd78) encoding the CM16 protein (chloroform/methanol-soluble protein) was isolated and characterized. To our knowledge this is the first characterization of a clone coding for a wheat CM protein. The CM16 protein is synthesized as a preprotein with a signal peptide of 24 residues, the molecular weight of the mature protein being 13,438 Da. As other members of the cereal trypsin/alpha-amylase inhibitor family, the CM16 protein contains 10 cysteine residues, their position being well conserved. In developing endosperm the highest level of CM16 mRNA was detected at mid-maturation.
- Henrissat B, Popineau Y, Kader JC
- Hydrophobic-cluster analysis of plant protein sequences. A domain homology between storage and lipid-transfer proteins.
- Biochem J. 1988; 255: 901-5
- Display abstract
- Hydrophobic-cluster analysis was used to characterize a conserved domain located near the C-terminal amino acid sequence of wheat (Triticum aestivum) storage proteins. This domain was transformed into a linear template for a global search for similarities in over 5200 protein sequences. In addition to proteins that had already been found to exhibit homology to wheat storage proteins, a previously unreported homology was found with non-specific lipid-transfer proteins from castor bean (Ricinus communis) and from spinach (Spinacia oleracea) leaf. Hydrophobic-cluster analysis of various members of the present protein group clearly shows a typical domain structure where (i) variable and conserved domains are located along the sequence at precise positions, (ii) the conserved domains probably reflect a common ancestor, and (iii) the unique properties of a given protein (chain cut into subunits, repetitive domains, trypsin-inhibitor active site) are associated with the variable domains.
Structure (3D structures containing this domain)

3D Structures of AAI domains in PDB

PDB code	Main view	Title
1afh		Lipid transfer protein from maize seedlings, nmr, 15 structures
1b1u		Crystal structure of the bifunctional inhibitor ragi
1be2		Lipid transfer protein complexed with palmitate, nmr, 10 structures
1bea		Bifunctional hageman factor/amylase inhibitor from maize
1bfa		Recombinant bifunctional hageman factor/amylase inhibitor from maize
1bip		Bifunctional proteinase inhibitor trypsin/a-amylase from seeds of ragi (eleusine coracana gaertneri)
1bv2		Lipid transfer protein from rice seeds, nmr, 14 structures
1bwo		The crystal structure of wheat non-specific transfer protein complexed with two molecules of phospholipid at 2.1 a resolution
1cz2		Solution structure of wheat ns-ltp complexed with prostaglandin b2.
1fk0		Structural basis of non-specific lipid binding in maize lipid-transfer protein complexes with capric acid revealed by high-resolution x-ray crystallography
1fk1		Structural basis of non-specific lipid binding in maize lipid-transfer protein complexes with lauric acid revealed by high-resolution x-ray crystallography
1fk2		Structural basis of non-specific lipid binding in maize lipid-transfer protein complexes with myristic acid revealed by high-resolution x-ray crystallography
1fk3		Structural basis of non-specific lipid binding in maize lipid-transfer protein complexes with palmitoleic acid revealed by high-resolution x-ray crystallography
1fk4		Structural basis of non-specific lipid binding in maize lipid-transfer protein complexes with stearic acid revealed by high-resolution x-ray crystallography
1fk5		Structural basis of non-specific lipid binding in maize lipid-transfer protein complexes with oleic acid revealed by high-resolution x-ray crystallography
1fk6		Structural basis of non-specific lipid binding in maize lipid-transfer protein complexes with alpha-linolenic acid revealed by high-resolution x-ray crystallography
1fk7		Structural basis of non-specific lipid binding in maize lipid-transfer protein complexes with ricinoleic acid revealed by high-resolution x-ray crystallography
1gh1		Nmr structures of wheat nonspecific lipid transfer protein
1hss		0.19 alpha-amylase inhibitor from wheat
1hyp		Crystal structure of hydrophobic protein from soybean; a member of a new cystine-rich family
1jtb		Lipid transfer protein complexed with palmitoyl coenzyme a, nmr, 16 structures
1l6h		Solution structure of plant nsltp2 purified from rice (oryza sativa)
1lip		Barley lipid transfer protein (nmr, 4 structures)
1mid		Non-specific lipid transfer protein 1 from barley in complex with l-alfa-lysophosphatidylcholine, laudoyl
1mzl		Maize nonspecific lipid transfer protein
1mzm		Maize nonspecific lipid transfer protein complexed with palmitate
1n89		Solution structure of a liganded type 2 wheat non-specific lipid transfer protein
1pnb		Structure of napin bnib, nmr, 10 structures
1psy		Structure of ricc3, nmr, 20 structures
1rzl		Rice nonspecific lipid transfer protein
1s6d		Structure in solution of a methionine-rich 2s albumin protein from sunflower seed
1siy		Nmr structure of mung bean non-specific lipid transfer protein 1
1sm7		Solution structure of the recombinant pronapin precursor, bnib.
1t12		Solution structure of a new ltp1
1tmq		Structure of tenebrio molitor larval alpha-amylase in complex with ragi bifunctional inhibitor
1tuk		Crystal stucture of liganted type 2 non specific lipid transfer protein from wheat
1uva		Lipid binding in rice nonspecific lipid transfer protein-1 complexes from oryza sativa
1uvb		Lipid binding in rice nonspecific lipid transfer protein-1 complexes from oryza sativa
1uvc		Lipid binding in rice nonspecific lipid transfer protein-1 complexes from oryza sativa
1w2q		Allergen arah6 from peanut (arachis hypogaea)
2alg		Crystal structure of peach pru p3, the prototypic member of the family of plant non-specific lipid transfer protein pan-allergens
2b5s		Crystal structure of peach pru p3, the prototypic member of the family of plant non-specific lipid transfer protein pan-allergens
2ds2		Crystal structure of mabinlin ii
2rkn		X-ray structure of the self-defense and signaling protein dir1 from arabidopsis taliana
3cee		Three-dimensional structure of a post translational modified barley ltp1

Links (links to other resources describing this domain)
PFAM tryp_alpha_amyl
INTERPRO IPR016140
PROSITE CEREAL_TRYP_AMYL_INH

PFAM	tryp_alpha_amyl
INTERPRO	IPR016140
PROSITE	CEREAL_TRYP_AMYL_INH