Schultz et al. (1998) Proc. Natl. Acad. Sci. USA 95, 5857-5864
Letunic et al. (2012) Nucleic Acids Res , doi:10.1093/nar/gkr931

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Glyco_25 Glycosyl hydrolases family 25

SMART accession number:	SM00641
Description:
Interpro abstract (IPR018077):	O-Glycosyl hydrolases (EC 3.2.1.) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [(PUBMED:7624375), (PUBMED:8535779)]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site. Glycoside hydrolase family 25 comprises enzymes with only one known activity; lysozyme (EC 3.2.1.17). It has been shown [(PUBMED:1916274), (PUBMED:1747104)] that a number of cell-wall lytic enzymes are evolutionary related and can be classified into a single family. Two residues, an aspartate and a glutamate, have been shown [(PUBMED:567645)] to be important for the catalytic activity of the Charalopsis enzyme. These residues as well as some others in their vicinity are conserved in all proteins from this family.
Family alignment:	View or CHROMA formatCLUSTALW formatMSF formatFASTA formatPIR format

There are 672 Glyco_25 domains in 621 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Click on to expand nodes. To display all proteins with a Glyco_25 domain in a specific node, click on it.

  This tree shows only several representative species. The complete taxonomic breakdown of all proteins with Glyco_25 domain is also avaliable.

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• Metabolism (metabolic pathways involving proteins which contain this domain)

• Click the image to view the interactive version of the map in iPath

  % proteins involved KEGG pathway ID Description 100.00 map00530 Aminosugars metabolism This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with Glyco_25 domain which could be assigned to a KEGG orthologous group, and not all proteins containing Glyco_25 domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

• Structure (3D structures containing this domain)

• 3D Structures of Glyco_25 domains in PDB

  PDB code	Main view	Title
  1h09		Multimodular pneumococcal cell wall endolysin from phage cp-1
  1jfx		Crystal structure of the bacterial lysozyme from streptomyces coelicolor at 1.65 a resolution
  1oba		Multimodular pneumococcal cell wall endolysin from phage cp-1 complexed with choline
  2ixu		Crystal structure of the modular cpl-1 endolysin complexed with a peptidoglycan analogue (wild-type endolysin)
  2ixv		Crystal structure of the modular cpl-1 endolysin complexed with a peptidoglycan analogue (e94q mutant)
  2j8f		Crystal structure of the modular cpl-1 endolysin complexed with a peptidoglycan analogue (e94q mutant in complex with a disaccharide-pentapeptide)
  2j8g		Crystal structure of the modular cpl-1 endolysin complexed with a peptidoglycan analogue (e94q mutant in complex with a tetrasaccharide-pentapeptide)
  2wag		The structure of a family 25 glycosyl hydrolase from bacillus anthracis.

• Links (links to other resources describing this domain)

• PFAM	Glyco_hydro_25
  INTERPRO	IPR018077

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