CAP10Putative lipopolysaccharide-modifying enzyme. |
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| SMART accession number: | SM00672
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| Description: |
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| Interpro abstract (IPR006598): |
Cryptococcus neoformans is a pathogenic fungus which most commonly affects the central nervous system and causes fatal meningoencephalitis primarily in patients with AIDS. This fungus produces a thick extracellular polysaccharide capsule which is well recognised as a virulence factor. CAP10 is required for capsule formation and virulence [(PUBMED:10482503)].
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There are 488
CAP10 domains in 488 proteins in SMART's nrdb database.
Click on the following links for more information.
- Evolution (species in which this domain is found)
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- Cellular role (predicted cellular role)
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Binding / catalysis: Lipopolysaccharide-modification?
- Literature (relevant references for this domain)
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Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.
- Chang YC, Kwon-Chung KJ
- Isolation, characterization, and localization of a capsule-associatedgene, CAP10, of Cryptococcus neoformans.
- J Bacteriol. 1999; 181: 5636-43
- Display abstract
Cryptococcus neoformans is a pathogenic fungus which most commonly affectsthe central nervous system and causes fatal meningoencephalitis primarilyin patients with AIDS. This fungus produces a thick extracellularpolysaccharide capsule which is well recognized as a virulence factor.Here, we describe the isolation and characterization of a novel gene,CAP10, which is required for capsule formation. Complementation of theacapsular cap10 mutant produced an encapsulated strain and the deletion ofCAP10 from a wild strain resulted in an acapsular phenotype. The molecularmass of the hemagglutinin epitope-tagged Cap10p is about 73 kDa, which issimilar to the size predicted from sequence analysis. When CAP10 was fusedwith a hybrid green fluorescent protein construct, the fluorescencesignals appeared as patches in the cytoplasm. Using a reporter geneconstruct, we found that CAP10 was expressed at high levels inlate-stationary-phase cells. In addition, we found that the expressionlevels of CAP10 are modulated by the transcriptional factor STE12alpha.Deletion of STE12alpha downregulated the expression levels of CAP10 whileoverexpression of STE12alpha upregulated the expression levels of CAP10.Animal model studies indicate that deletion of the CAP10 gene results inthe loss of virulence, and complementation of the acapsular phenotype ofcap10 restores virulence. Thus, CAP10 is required for capsule formationand virulence.
- Links (links to other resources describing this domain)
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