CENPB

Putative DNA-binding domain in centromere protein B, mouse jerky and transposases.
CENPB
SMART accession number:SM00674
Description: -
Interpro abstract (IPR006600):

The CENPB-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of about 70-75 amino acids, present in eukaryotic centromere proteins and transposases. The domain is named after the mammalian major centromere autoantigen B or centromere protein B (CENP-B), which is a fundamental centromere component of chromosomes. The N terminus of CENP-B contains two DNA-binding HTH domains, which bind to adjacent major grooves of DNA. The N terminus of CENP-B is formed by a psq-type HTH domain and C-terminal to this domain lies the CENPB-type HTH domain. These two HTH domains together bind specifically to a 17-base-pair sequence, the CENP-B box, which occurs in alpha-satellite DNA in human centromeres [(PUBMED:16183641)].

The structure of the CENPB-type HTH domain is composed of three alpha-helices. The second and third helices connected via a turn comprise the helix-turn-helix motif. Helix 3 is termed the recognition helix as it binds the DNA major groove, like in other HTHs. In CENP-B this domain recognises site 3 of the CENP-B box, while the preceding psq-type HTH binds site 1 of the CENP-B box, and a connecting linker loop binds in the minor groove of DNA and recognises site 2 [(PUBMED:11726497)].

Some proteins known to contain a CENPB-type HTH domain:

  • Mammalian centromere protein B (CENP-B), associated with the centromere and specifically binding DNA to the CENP-B box.
  • Mammalian jerky protein, involved in epileptic seizures in mice [(PUBMED:15487591)].
  • Mammalian Pogo transposases [(PUBMED:9862965)] and tigger transposable elements [(PUBMED:9097724)].
  • Fission yeast ARS-binding protein 1 (abp1) [(PUBMED:17112379)] and CENP-B homologue proteins (CBHP-1 and 2), which are centromere proteins [(PUBMED:9237993), (PUBMED:11238404)].
  • Candida albicans protein PDC2 (Pyruvate DeCarboxylase 2) [(PUBMED:8264540), (PUBMED:19013460)].
  • Fungal transposases.
Family alignment:
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There are 2646 CENPB domains in 2609 proteins in SMART's nrdb database.

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