Letunic et al. (2017) Nucleic Acids Res doi: 10.1093/nar/gkx922
Letunic et al. (2020) Nucleic Acids Res doi: 10.1093/nar/gkaa937

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Resolvase Resolvase, N terminal domain

SMART accession number:	SM00857
Description:	The N-terminal domain of the resolvase family contains the active site and the dimer interface. The extended arm at the C-terminus of this domain connects to the C-terminal helix-turn-helix domain of resolvase.
Interpro abstract (IPR006119):	Site-specific recombination plays an important role in DNA rearrangement in prokaryotic organisms. Two types of site-specific recombination are known to occur: Recombination between inverted repeats resulting in the reversal of a DNA segment. Recombination between repeat sequences on two DNA molecules resulting in their cointegration, or between repeats on one DNA molecule resulting in the excision of a DNA fragment. Site-specific recombination is characterised by a strand exchange mechanism that requires no DNA synthesis or high energy cofactor; the phosphodiester bond energy is conserved in a phospho-protein linkage during strand cleavage and re-ligation. Two unrelated families of recombinases are currently known [ (PUBMED:3011407) ]. The first, called the 'phage integrase' family, groups a number of bacterial phage and yeast plasmid enzymes. The second [ (PUBMED:2896291) ], called the 'resolvase' family, groups enzymes which share the following structural characteristics: an N-terminal catalytic and dimerization domain that contains a conserved serine residue involved in the transient covalent attachment to DNA, and a C-terminal helix-turn-helix DNA-binding domain ( IPR006120 ). The N-terminal resolvase/invertase-type recombinase catalytic domain has an alpha/beta fold and consists of a five-stranded mixed beta-sheet surrounded by three alpha helices on one side and one helix on the other [ (PUBMED:7628011) ].
GO process:	DNA recombination (GO:0006310)
GO function:	DNA binding (GO:0003677), recombinase activity (GO:0000150)
Family alignment:	View or CHROMA formatHMM/Web logoCLUSTALW formatMSF formatFASTA formatPIR format

There are 61235 Resolvase domains in 61219 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Taxonomic distribution of proteins containing Resolvase domain.

  This tree includes only several representative species. The complete taxonomic breakdown of all proteins with Resolvase domain is also avaliable.

  Click on the protein counts, or double click on taxonomic names to display all proteins containing Resolvase domain in the selected taxonomic class.

• Cellular role (predicted cellular role)

• Cellular role: chromatin
  

• Structure (3D structures containing this domain)

• 3D Structures of Resolvase domains in PDB

  PDB code	Main view	Title
  1gdr		MODEL FOR A DNA MEDIATED SYNAPTIC COMPLEX SUGGESTED BY CRYSTAL PACKING OF GAMMA DELTA RESOLVASE SUBUNITS
  1gdt		CRYSTAL STRUCTURE OF A SITE-SPECIFIC RECOMBINASE, GAMMA-DELTA RESOLVASE COMPLEXED WITH A 34 BP CLEAVAGE SITE
  1ght		SOLUTION STRUCTURE OF THE CATALYTIC DOMAIN OF GAMMA DELTA RESOLVASE
  1hx7		SOLUTION STRUCTURE OF THE CATALYTIC DOMAIN OF GAMMA DELTA RESOLVASE
  1zr2		Structure of a Synaptic gamma-delta Resolvase Tetramer Covalently Linked to two Cleaved DNAs
  1zr4		Structure of a Synaptic gamma-delta Resolvase Tetramer Covalently linked to two Cleaved DNAs
  2gm4		An activated, tetrameric gamma-delta resolvase: Hin chimaera bound to cleaved DNA
  2gm5		An activated, truncated gamma-delta resolvase tetramer
  2mhc		2MHC
  2r0q		Crystal structure of a serine recombinase- DNA regulatory complex
  2rsl		REFINEMENT OF GAMMA DELTA RESOLVASE REVEALS A STRIKINGLY FLEXIBLE MOLECULE
  3bvp		Crystal Structure of the N-terminal Catalytic Domain of TP901-1 Integrase
  3g13		Crystal structure of putative conjugative transposon recombinase from Clostridium difficile
  3guv		Crystal structure of a resolvase family site-specific recombinase from Streptococcus pneumoniae
  3ilx		Crystal structure of First ORF in transposon ISC1904 from Sulfolobus solfataricus P2
  3lhf		The Crystal Structure of a Serine Recombinase from Sulfolobus solfataricus to 2.3A
  3lhk		Crystal structure of putative DNA binding protein from Methanocaldococcus jannaschii.
  3pkz		Structural basis for catalytic activation of a serine recombinase
  3uj3		Crystal Structure of the synaptic tetramer of the G-Segment Invertase (Gin)
  4bqq		Protein crystal structure of the N-terminal and recombinase domains of the Streptomyces temperate phage serine recombinase, fC31 integrase.
  4m6f		Dimer of the G-Segment Invertase bound to a DNA substrate

• Links (links to other resources describing this domain)

• INTERPRO	IPR006119
  PFAM	Resolvase

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