UreE_CUreE urease accessory protein, C-terminal domain |
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| SMART accession number: | SM00987 |
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| Description: | UreE is a urease accessory protein. Urease hydrolyses urea into ammonia and carbamic acid. The C-terminal region of members of this family contains a His rich Nickel binding site. |
| Family alignment: |
There are 3634 UreE_C domains in 3634 proteins in SMART's nrdb database.
Click on the following links for more information.
- Evolution (species in which this domain is found)
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Go to specific node: Anopheles gambiae, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae, Takifugu rubripes - Cellular role (predicted cellular role)
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Cellular role: metabolism
- Literature (relevant references for this domain)
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Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.
- Song HK, Mulrooney SB, Huber R, Hausinger RP
- Crystal structure of Klebsiella aerogenes UreE, a nickel-bindingmetallochaperone for urease activation.
- J Biol Chem. 2001; 276: 49359-64
- Display abstract
UreE is proposed to be a metallochaperone that delivers nickel ions tourease during activation of this bacterial virulence factor. Wild-typeKlebsiella aerogenes UreE binds approximately six nickel ions perhomodimer, whereas H144*UreE (a functional C-terminal truncated variant)was previously reported to bind two. We determined the structure ofH144*UreE by multi-wavelength anomalous diffraction and refined it to 1.5A resolution. The present structure reveals an Hsp40-like peptide-bindingdomain, an Atx1-like metal-binding domain, and a flexible C terminus.Three metal-binding sites per dimer, defined by structural analysis ofCu-H144*UreE, are on the opposite face of the Atx1-like domain thanobserved in the copper metallochaperone. One metal bridges the twosubunits via the pair of His-96 residues, whereas the other two sitesinvolve metal coordination by His-110 and His-112 within each subunit. Incontrast to the copper metallochaperone mechanism involving thiol ligandexchanges between structurally similar chaperones and target proteins, wepropose that the Hsp40-like module interacts with urease apoprotein and/orother urease accessory proteins, while the Atx1-like domain delivershistidyl-bound nickel to the urease active site.
- Structure (3D structures containing this domain)
3D Structures of UreE_C domains in PDB
PDB code Main view Title 1akz 
Human uracil-dna glycosylase 1emh 
Crystal structure of human uracil-dna glycosylase bound to uncleaved substrate-containing dna 1emj 
Uracil-dna glycosylase bound to dna containing a 4'-thio- 2'deoxyuridine analog product 1eug 
Crystal structure of escherichia coli uracil dna glycosylase and its complexes with uracil and glycerol: structure and glycosylase mechanism revisited 1eui 
Escherichia coli uracil-dna glycosylase complex with uracil- dna glycosylase inhibitor protein 1flz 
Uracil dna glycosylase with uaap 1l9g 
Crystal structure of uracil-dna glycosylase from t. maritima 1lau 
Uracil-dna glycosylase 1lqg 
Escherichia coli uracil-dna glycosylase complex with uracil- dna glycosylase inhibitor protein 1lqj 
Escherichia coli uracil-dna glycosylase 1lqm 
Escherichia coli uracil-dna glycosylase complex with uracil- dna glycosylase inhibitor protein 1mtl 
Non-productive mug-dna complex 1mug 
G:t/u mismatch-specific dna glycosylase from e.coli 1mwi 
Crystal structure of a mug-dna product complex 1mwj 
Crystal structure of a mug-dna pseudo substrate complex 1okb 
Crystal structure of uracil-dna glycosylase from atlantic cod (gadus morhua) 1q3f 
Uracil dna glycosylase bound to a cationic 1-aza-2'- deoxyribose-contianing dna 1ssp 
Wild-type uracil-dna glycosylase bound to uracil-containing dna 1udg 
The structural basis of specific base excision repair by uracil-dna glycosylase 1udh 
The structural basis of specific base excision repair by uracil-dna glycosylase 1udi 
Nucleotide mimicry in the crystal structure of the uracil- dna glycosylase-uracil glycosylase inhibitor protein complex 1ugh 
Crystal structure of human uracil-dna glycosylase in complex with a protein inhibitor: protein mimicry of dna 1ui0 
Crystal structure of uracil-dna glycosylase from thermus thermophilus hb8 1ui1 
Crystal structure of uracil-dna glycosylase from thermus thermophilus hb8 1uug 
Escherichia coli uracil-dna glycosylase:inhibitor complex with wild-type udg and wild-type ugi 1vk2 
Crystal structure of uracil-dna glycosylase (tm0511) from thermotoga maritima at 1.90 a resolution 1wyw 
Crystal structure of sumo1-conjugated thymine dna glycosylase 1yuo 
Optimisation of the surface electrostatics as a strategy for cold adaptation of uracil-dna n-glycosylase (ung)from atlantic cod (gadus morhua) 2boo 
The crystal structure of uracil-dna n-glycosylase (ung) from deinococcus radiodurans. 2c53 
A comparative study of uracil dna glycosylases from human and herpes simplex virus type 1 2c56 
A comparative study of uracil dna glycosylases from human and herpes simplex virus type 1 2d07 
Crystal structure of sumo-3-modified thymine-dna glycosylase 2d3y 
Crystal structure of uracil-dna glycosylase from thermus thermophilus hb8 2ddg 
Crystal structure of uracil-dna glycosylase in complex with ap:g containing dna 2dem 
Crystal structure of uracil-dna glycosylase in complex with ap:a containing dna 2dp6 
Crystal structure of uracil-dna glycosylase in complex with ap:c containing dna 2eug 
Crystal structure of escherichia coli uracil dna glycosylase and its complexes with uracil and glycerol: structure and glycosylase mechanism revisited 2hxm 
Complex of ung2 and a small molecule synthetic inhibitor 2j8x 
Epstein-barr virus uracil-dna glycosylase in complex with ugi from pbs-2 2jhq 
Crystal structure of uracil dna-glycosylase from vibrio cholerae 2oxm 
Crystal structure of a ung2/modified dna complex that represent a stabilized short-lived extrahelical state in ezymatic dna base flipping 2oyt 
Crystal structure of ung2/dna(tm) 2rba 
Structure of human thymine dna glycosylase bound to abasic and undamaged dna 2ssp 
Leucine-272-alanine uracil-dna glycosylase bound to abasic site-containing dna 2uug 
Escherichia coli uracil-dna glycosylase:inhibitor complex with h187d mutant udg and wild-type ugi 2zhx 
Crystal structure of uracil-dna glycosylase from mycobacterium tuberculosis in complex with a proteinaceous inhibitor 3cxm 
Leishmania naiffi uracil-dna glycosylase in complex with 5- bromouracil 3eug 
Crystal structure of escherichia coli uracil dna glycosylase and its complexes with uracil and glycerol: structure and glycosylase mechanism revisited 3fcf 
Complex of ung2 and a fragment-based designed inhibitor 3fci 
Complex of ung2 and a fragment-based designed inhibitor 3fck 
Complex of ung2 and a fragment-based design inhibitor 3fcl 
Complex of ung2 and a fragment-based designed inhibitor 3ikb 
The structure of a conserved protein from streptococcus mutans ua159. 4eug 
Crystallographic and enzymatic studies of an active site variant h187q of escherichia coli uracil dna glycosylase: crystal structures of mutant h187q and its uracil complex 4skn 
A nucleotide-flipping mechanism from the structure of human uracil-dna glycosylase bound to dna 5eug 
Crystallographic and enzymatic studies of an active site variant h187q of escherichia coli uracil dna glycosylase: crystal structures of mutant h187q and its uracil complex - Links (links to other resources describing this domain)
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PFAM UreE_C