UreE_CUreE urease accessory protein, C-terminal domain |
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| SMART accession number: | SM00987 |
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| Description: | UreE is a urease accessory protein. Urease hydrolyses urea into ammonia and carbamic acid. The C-terminal region of members of this family contains a His rich Nickel binding site. |
| Family alignment: |
There are 48577 UreE_C domains in 48571 proteins in SMART's nrdb database.
Click on the following links for more information.
- Evolution (species in which this domain is found)
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Taxonomic distribution of proteins containing UreE_C domain.
This tree includes only several representative species. The complete taxonomic breakdown of all proteins with UreE_C domain is also avaliable.
Click on the protein counts, or double click on taxonomic names to display all proteins containing UreE_C domain in the selected taxonomic class.
- Cellular role (predicted cellular role)
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Cellular role: metabolism
- Literature (relevant references for this domain)
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Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.
- Song HK, Mulrooney SB, Huber R, Hausinger RP
- Crystal structure of Klebsiella aerogenes UreE, a nickel-bindingmetallochaperone for urease activation.
- J Biol Chem. 2001; 276: 49359-64
- Display abstract
UreE is proposed to be a metallochaperone that delivers nickel ions tourease during activation of this bacterial virulence factor. Wild-typeKlebsiella aerogenes UreE binds approximately six nickel ions perhomodimer, whereas H144*UreE (a functional C-terminal truncated variant)was previously reported to bind two. We determined the structure ofH144*UreE by multi-wavelength anomalous diffraction and refined it to 1.5A resolution. The present structure reveals an Hsp40-like peptide-bindingdomain, an Atx1-like metal-binding domain, and a flexible C terminus.Three metal-binding sites per dimer, defined by structural analysis ofCu-H144*UreE, are on the opposite face of the Atx1-like domain thanobserved in the copper metallochaperone. One metal bridges the twosubunits via the pair of His-96 residues, whereas the other two sitesinvolve metal coordination by His-110 and His-112 within each subunit. Incontrast to the copper metallochaperone mechanism involving thiol ligandexchanges between structurally similar chaperones and target proteins, wepropose that the Hsp40-like module interacts with urease apoprotein and/orother urease accessory proteins, while the Atx1-like domain delivershistidyl-bound nickel to the urease active site.
- Structure (3D structures containing this domain)
3D Structures of UreE_C domains in PDB
PDB code Main view Title 1akz 
HUMAN URACIL-DNA GLYCOSYLASE 1emh 
CRYSTAL STRUCTURE OF HUMAN URACIL-DNA GLYCOSYLASE BOUND TO UNCLEAVED SUBSTRATE-CONTAINING DNA 1emj 
URACIL-DNA GLYCOSYLASE BOUND TO DNA CONTAINING A 4'-THIO-2'DEOXYURIDINE ANALOG PRODUCT 1eug 
CRYSTAL STRUCTURE OF ESCHERICHIA COLI URACIL DNA GLYCOSYLASE AND ITS COMPLEXES WITH URACIL AND GLYCEROL: STRUCTURE AND GLYCOSYLASE MECHANISM REVISITED 1eui 
ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE COMPLEX WITH URACIL-DNA GLYCOSYLASE INHIBITOR PROTEIN 1flz 
URACIL DNA GLYCOSYLASE WITH UAAP 1l9g 
CRYSTAL STRUCTURE OF URACIL-DNA GLYCOSYLASE FROM T. MARITIMA 1lau 
URACIL-DNA GLYCOSYLASE 1lqg 
ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE COMPLEX WITH URACIL-DNA GLYCOSYLASE INHIBITOR PROTEIN 1lqj 
ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE 1lqm 
ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE COMPLEX WITH URACIL-DNA GLYCOSYLASE INHIBITOR PROTEIN 1mtl 
Non-productive MUG-DNA complex 1mug 
G:T/U MISMATCH-SPECIFIC DNA GLYCOSYLASE FROM E.COLI 1mwi 
Crystal structure of a MUG-DNA product complex 1mwj 
Crystal Structure of a MUG-DNA pseudo substrate complex 1okb 
crystal structure of Uracil-DNA glycosylase from Atlantic cod (Gadus morhua) 1q3f 
Uracil DNA glycosylase bound to a cationic 1-aza-2'-deoxyribose-contianing DNA 1ssp 
WILD-TYPE URACIL-DNA GLYCOSYLASE BOUND TO URACIL-CONTAINING DNA 1udg 
THE STRUCTURAL BASIS OF SPECIFIC BASE EXCISION REPAIR BY URACIL-DNA GLYCOSYLASE 1udh 
THE STRUCTURAL BASIS OF SPECIFIC BASE EXCISION REPAIR BY URACIL-DNA GLYCOSYLASE 1udi 
NUCLEOTIDE MIMICRY IN THE CRYSTAL STRUCTURE OF THE URACIL-DNA GLYCOSYLASE-URACIL GLYCOSYLASE INHIBITOR PROTEIN COMPLEX 1ugh 
CRYSTAL STRUCTURE OF HUMAN URACIL-DNA GLYCOSYLASE IN COMPLEX WITH A PROTEIN INHIBITOR: PROTEIN MIMICRY OF DNA 1ui0 
Crystal Structure Of Uracil-DNA Glycosylase From Thermus Thermophilus HB8 1ui1 
Crystal Structure Of Uracil-DNA Glycosylase From Thermus Thermophilus HB8 1uug 
ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE:INHIBITOR COMPLEX WITH WILD-TYPE UDG AND WILD-TYPE UGI 1vk2 
Crystal structure of Uracil-DNA glycosylase (TM0511) from Thermotoga maritima at 1.90 A resolution 1wyw 
Crystal Structure of SUMO1-conjugated thymine DNA glycosylase 1yuo 
Optimisation of the surface electrostatics as a strategy for cold adaptation of uracil-DNA N-glycosylase (UNG)from atlantic cod (Gadus morhua) 2boo 
The crystal structure of Uracil-DNA N-Glycosylase (UNG) from Deinococcus radiodurans. 2c53 
A comparative study of uracil DNA glycosylases from human and herpes simplex virus type 1 2c56 
A comparative study of uracil DNA glycosylases from human and herpes simplex virus type 1 2d07 
Crystal Structure of SUMO-3-modified Thymine-DNA Glycosylase 2d3y 
Crystal structure of uracil-DNA glycosylase from Thermus Thermophilus HB8 2ddg 
Crystal structure of uracil-DNA glycosylase in complex with AP:G containing DNA 2dem 
Crystal structure of Uracil-DNA glycosylase in complex with AP:A containing DNA 2dp6 
Crystal structure of uracil-DNA glycosylase in complex with AP:C containing DNA 2eug 
CRYSTAL STRUCTURE OF ESCHERICHIA COLI URACIL DNA GLYCOSYLASE AND ITS COMPLEXES WITH URACIL AND GLYCEROL: STRUCTURE AND GLYCOSYLASE MECHANISM REVISITED 2hxm 
Complex of UNG2 and a small Molecule synthetic Inhibitor 2j8x 
Epstein-Barr virus uracil-DNA glycosylase in complex with Ugi from PBS-2 2jhq 
Crystal structure of Uracil DNA-glycosylase from Vibrio cholerae 2l3f 
Solution NMR Structure of a putative Uracil DNA glycosylase from Methanosarcina acetivorans, Northeast Structural Genomics Consortium Target MvR76 2oxm 
Crystal structure of a UNG2/modified DNA complex that represent a stabilized short-lived extrahelical state in ezymatic DNA base flipping 2oyt 
Crystal Structure of UNG2/DNA(TM) 2rba 
Structure of Human Thymine DNA Glycosylase Bound to Abasic and Undamaged DNA 2ssp 
LEUCINE-272-ALANINE URACIL-DNA GLYCOSYLASE BOUND TO ABASIC SITE-CONTAINING DNA 2uug 
ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE:INHIBITOR COMPLEX WITH H187D MUTANT UDG AND WILD-TYPE UGI 2zhx 
Crystal structure of Uracil-DNA Glycosylase from Mycobacterium tuberculosis in complex with a proteinaceous inhibitor 3a7n 
Crystal structure of uracil-DNA glycosylase from Mycobacterium tuberculosis 3cxm 
Leishmania naiffi uracil-DNA glycosylase in complex with 5-bromouracil 3eug 
CRYSTAL STRUCTURE OF ESCHERICHIA COLI URACIL DNA GLYCOSYLASE AND ITS COMPLEXES WITH URACIL AND GLYCEROL: STRUCTURE AND GLYCOSYLASE MECHANISM REVISITED 3fcf 
Complex of UNG2 and a fragment-based designed inhibitor 3fci 
Complex of UNG2 and a fragment-based designed inhibitor 3fck 
Complex of UNG2 and a fragment-based design inhibitor 3fcl 
Complex of UNG2 and a fragment-based designed inhibitor 3ikb 
The structure of a conserved protein from Streptococcus mutans UA159. 3tkb 
crystal structure of human uracil-DNA glycosylase D183G/K302R mutant 3tr7 
Structure of a uracil-DNA glycosylase (ung) from Coxiella burnetii 3uf7 
Co-crystal structure of Escherichia coli uracil-DNA glycosylase and a C-terminal fragement of the single-stranded DNA-binding protein 3ufj 
Human Thymine DNA Glycosylase Bound to Substrate Analog 2'-fluoro-2'-deoxyuridine 3ufm 
Co-crystal structure of Deinococcus radiodurans uracil-DNA glycosylase and the C-terminus of the single-stranded DNA-binding protein 3uo7 
Crystal structure of Human Thymine DNA Glycosylase Bound to Substrate 5-carboxylcytosine 3uob 
Crystal structure of Human Thymine DNA Glycosylase Bound to Substrate Analog 2'-deoxy-2'-beta-fluoro-cytidine 3wdf 
Staphylococcus aureus UDG 3wdg 
Staphylococcus aureus UDG / UGI complex 3zoq 
Structure of BsUDG-p56 complex 3zor 
Structure of BsUDG 4eug 
Crystallographic and Enzymatic Studies of an Active Site Variant H187Q of Escherichia Coli Uracil DNA Glycosylase: Crystal Structures of Mutant H187Q and its Uracil Complex 4fnc 
Human TDG in a post-reactive complex with 5-hydroxymethyluracil (5hmU) 4jgc 
Human TDG N140A mutant IN A COMPLEX WITH 5-carboxylcytosine (5caC) 4l5n 
Crystallographic Structure of HHV-1 Uracil-DNA Glycosylase complexed with the Bacillus phage PZA inhibitor protein p56 4lyl 
4LYL 4skn 
A NUCLEOTIDE-FLIPPING MECHANISM FROM THE STRUCTURE OF HUMAN URACIL-DNA GLYCOSYLASE BOUND TO DNA 4uqm 
4UQM 4wpk 
4WPK 4wpl 
4WPL 4wru 
4WRU 4wrv 
4WRV 4wrw 
4WRW 4wrx 
4WRX 4wry 
4WRY 4wrz 
4WRZ 4ws0 
4WS0 4ws1 
4WS1 4ws2 
4WS2 4ws3 
4WS3 4ws4 
4WS4 4ws5 
4WS5 4ws6 
4WS6 4ws7 
4WS7 4ws8 
4WS8 4xeg 
4XEG 4z3a 
4Z3A 4z47 
4Z47 4z7b 
4Z7B 4z7z 
4Z7Z 4zbx 
4ZBX 4zby 
4ZBY 4zbz 
4ZBZ 5ayr 
5AYR 5ays 
5AYS 5cys 
5CYS 5eug 
CRYSTALLOGRAPHIC AND ENZYMATIC STUDIES OF AN ACTIVE SITE VARIANT H187Q OF ESCHERICHIA COLI URACIL DNA GLYCOSYLASE: CRYSTAL STRUCTURES OF MUTANT H187Q AND ITS URACIL COMPLEX 5ff8 
5FF8 5h93 
5H93 5h98 
5H98 5h99 
5H99 5h9i 
5H9I 5hf7 
5HF7 5jk7 
5JK7 5jxy 
5JXY 5t2w 
5T2W - Links (links to other resources describing this domain)
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PFAM UreE_C