AlkA_N

AlkA N-terminal domain
AlkA_N
SMART accession number:SM01009
Description: This domain is found at the N terminus of bacterial AlkA . AlkA (3-methyladenine-DNA glycosylase II) is a base excision repair glycosylase from Escherichia coli. It removes a variety of alkylated bases from DNA, primarily by removing alkylation damage from duplex and single stranded DNA. AlkA flips a 1-azaribose abasic nucleotide out of DNA. This produces a 66 degrees bend in the DNA and a marked widening of the minor groove (PUBMED:10675345).
Interpro abstract (IPR010316):

AlkA (DNA-3-methyladenine glycosylase II) is a base excision repair glycosylase from Escherichia coli. It removes a variety of alkylated bases from DNA, primarily by removing alkylation damage from duplex and single stranded DNA. AlkA is similar in fold and active site location to the bifunctional glycosylase/lyase endonuclease III. This suggests that the two may use similar mechanisms for base excision [(PUBMED:8706136)]. The structural analysis of the AlkA and AlkA-hypoxanthine structures indicate that free hypoxanthine binding in the active site may inhibit glycosylase activity [(PUBMED:12009927)].

The AlkA protein consists of three domains: an N-terminal mixed alpha-beta structure, a central seven-helix bundle, and a C-terminal domain of four a helices [(PUBMED:8706136)]. This entry represents the N-terminal domain.

Family alignment:
View or

There are 1573 AlkA_N domains in 1573 proteins in SMART's nrdb database.

Click on the following links for more information.