Letunic et al. (2017) Nucleic Acids Res doi: 10.1093/nar/gkx922
Letunic et al. (2020) Nucleic Acids Res doi: 10.1093/nar/gkaa937

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PKS_TE Thioesterase

SMART accession number:	SM00824
Description:	Peptide synthetases are involved in the non-ribosomal synthesis of peptide antibiotics. Next to the operons encoding these enzymes, in almost all cases, are genes that encode proteins that have similarity to the type II fatty acid thioesterases of vertebrates. There are also modules within the peptide synthetases that also share this similarity. With respect to antibiotic production, thioesterases are required for the addition of the last amino acid to the peptide antibiotic, thereby forming a cyclic antibiotic. Thioesterases (non-integrated) have molecular masses of 25-29 kDa.
Interpro abstract (IPR020802):	Thioesterase domains often occur integrated in or associated with peptide synthetases which are involved in the non-ribosomal synthesis of peptide antibiotics [ (PUBMED:9560421) ]. Thioesterases are required for the addition of the last amino acid to the peptide antibiotic, thereby forming a cyclic antibiotic. Next to the operons encoding these enzymes, in almost all cases, are genes that encode proteins that have similarity to the type II fatty acid thioesterases of vertebrates.
GO process:	biosynthetic process (GO:0009058)
GO function:	hydrolase activity, acting on ester bonds (GO:0016788)
Family alignment:	View or CHROMA formatHMM/Web logoCLUSTALW formatMSF formatFASTA formatPIR format

There are 856 PKS_TE domains in 856 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Taxonomic distribution of proteins containing PKS_TE domain.

  This tree includes only several representative species. The complete taxonomic breakdown of all proteins with PKS_TE domain is also avaliable.

  Click on the protein counts, or double click on taxonomic names to display all proteins containing PKS_TE domain in the selected taxonomic class.

• Cellular role (predicted cellular role)

• Cellular role: metabolism
  

• Metabolism (metabolic pathways involving proteins which contain this domain)

• Click the image to view the interactive version of the map in iPath

  % proteins involved KEGG pathway ID Description 10.00 map00350 Tyrosine metabolism 10.00 map00564 Glycerophospholipid metabolism 10.00 map00960 Alkaloid biosynthesis II 10.00 map00903 Limonene and pinene degradation 10.00 map00632 Benzoate degradation via CoA ligation 10.00 map00310 Lysine degradation 10.00 map00624 1- and 2-Methylnaphthalene degradation 10.00 map00360 Phenylalanine metabolism 10.00 map00340 Histidine metabolism 10.00 map00642 Ethylbenzene degradation This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with PKS_TE domain which could be assigned to a KEGG orthologous group, and not all proteins containing PKS_TE domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

• Structure (3D structures containing this domain)

• 3D Structures of PKS_TE domains in PDB

  PDB code	Main view	Title
  1kez		Crystal Structure of the Macrocycle-forming Thioesterase Domain of Erythromycin Polyketide Synthase (DEBS TE)
  1mn6		Thioesterase Domain from Picromycin Polyketide Synthase, pH 7.6
  1mna		Thioesterase Domain of Picromycin Polyketide Synthase (PICS TE), pH 8.0
  1mnq		Thioesterase Domain of Picromycin Polyketide Synthase (PICS TE), pH 8.4
  1mo2		Thioesterase Domain from 6-Deoxyerythronolide Synthase (DEBS TE), pH 8.5
  2h7x		Pikromycin Thioesterase adduct with reduced triketide affinity label
  2h7y		Pikromycin Thioesterase with covalent affinity label
  2hfj		Pikromycin thioesterase with covalent pentaketide affinity label
  2hfk		Pikromycin thioesterase in complex with product 10-deoxymethynolide
  5d3k		5D3K
  5d3z		5D3Z

• Links (links to other resources describing this domain)

• PFAM	Thioesterase
  INTERPRO	IPR020802

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