Letunic et al. (2017) Nucleic Acids Res doi: 10.1093/nar/gkx922
Letunic et al. (2020) Nucleic Acids Res doi: 10.1093/nar/gkaa937

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CAP_GLY

SMART accession number:	SM01052
Description:	Cytoskeleton-associated proteins (CAPs) are involved in the organisation of microtubules and transportation of vesicles and organelles along the cytoskeletal network. A conserved motif, CAP-Gly, has been identified in a number of CAPs, including CLIP-170 and dynactins. The crystal structure of Caenorhabditis elegans F53F4.3 protein Q20728 CAP-Gly domain was recently solved (PUBMED:12221106). The domain contains three beta-strands. The most conserved sequence, GKNDG, is located in two consecutive sharp turns on the surface, forming the entrance to a groove (PUBMED:12221106).
Interpro abstract (IPR000938):	Cytoskeleton-associated proteins (CAPs) are involved in the organisation of microtubules and transportation of vesicles and organelles along the cytoskeletal network. A conserved glycine-rich domain, CAP-Gly, has been identified in a number of CAPs, including CLIP-170 and dynactins. The crystal structure of the Caenorhabditis elegans F53F4.3 protein CAP-Gly domain has been solved. The domain contains three beta-strands. The most conserved sequence, GKNDG, is located in two consecutive sharp turns on the surface, forming the entrance to a groove [ (PUBMED:12221106) ].
Family alignment:	View or CHROMA formatHMM/Web logoCLUSTALW formatMSF formatFASTA formatPIR format

There are 17076 CAP_GLY domains in 12534 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Taxonomic distribution of proteins containing CAP_GLY domain.

  This tree includes only several representative species. The complete taxonomic breakdown of all proteins with CAP_GLY domain is also avaliable.

  Click on the protein counts, or double click on taxonomic names to display all proteins containing CAP_GLY domain in the selected taxonomic class.

• Cellular role (predicted cellular role)

• Cellular role: transport
  

• Literature (relevant references for this domain)

• Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.

  • Li S et al.
  • Crystal structure of the cytoskeleton-associated protein glycine-rich(CAP-Gly) domain.
  • J Biol Chem. 2002; 277: 48596-601
  • Display abstract

  • Cytoskeleton-associated proteins (CAPs) are involved in the organizationof microtubules and transportation of vesicles and organelles along thecytoskeletal network. A conserved motif, CAP-Gly, has been identified in anumber of CAPs, including CLIP-170 and dynactins. The crystal structure ofthe CAP-Gly domain of Caenorhabditis elegans F53F4.3 protein, solved bysingle wavelength sulfur-anomalous phasing, revealed a novel protein foldcontaining three beta-sheets. The most conserved sequence, GKNDG, islocated in two consecutive sharp turns on the surface, forming theentrance to a groove. Residues in the groove are highly conserved asmeasured from the information content of the aligned sequences. TheC-terminal tail of another molecule in the crystal is bound in thisgroove.

  • Riehemann K, Sorg C
  • Sequence homologies between four cytoskeleton-associated proteins.
  • Trends Biochem Sci. 1993; 18: 82-3
• Structure (3D structures containing this domain)

• 3D Structures of CAP_GLY domains in PDB

  PDB code	Main view	Title
  1ixd		Solution structure of the CAP-GLY domain from human cylindromatosis tomour-suppressor CYLD
  1lpl		Structural Genomics of Caenorhabditis elegans: CAP-Gly domain of F53F4.3
  1tov		Structural genomics of Caenorhabditis elegans: CAP-GLY domain of F53F4.3
  1txq		Crystal structure of the EB1 C-terminal domain complexed with the CAP-Gly domain of p150Glued
  1whg		Solution structure of the CAP-Gly domain in mouse tubulin specific chaperone B
  1whh		Solution structure of the 2nd CAP-Gly domain in mouse CLIP170-related 59kDa protein CLIPR-59
  1whj		Solution structure of the 1st CAP-Gly domain in mouse 1700024K14Rik hypothetical protein
  1whk		Solution structure of the 3rd CAP-Gly domain in mouse 1700024K14Rik hypothetical protein
  1whl		Solution structure of the 1st CAP-Gly domain in human cylindromatosis tumor suppressor CYLD
  1whm		Solution structure of the 2nd CAP-Gly domain in human cylindromatosis tumor suppressor CYLD
  2cow		Solution structure of the CAP-Gly domain in human Kinesin-like protein KIF13B
  2coy		Solution structure of the CAP-Gly domain in human Dynactin 1
  2coz		Solution structure of the CAP-Gly domain in human centrosome-associated protein CAP350
  2cp0		Solution structure of the 1st CAP-Gly domain in human CLIP-170-related protein CLIPR59
  2cp2		Solution structure of the 1st CAP-Gly domain in human CLIP-115/CYLN2
  2cp3		Solution structure of the 2nd CAP-Gly domain in human CLIP-115/CYLN2
  2cp5		Solution structure of the 1st CAP-Gly domain in human CLIP-170/restin
  2cp6		Solution structure of the 2nd CAP-Gly domain in human CLIP-170/restin
  2cp7		Solution structure of the 1st CAP-Gly domain in mouse CLIP-170/restin
  2e3h		Crystal structure of the CLIP-170 CAP-Gly domain 2
  2e3i		Crystal structure of the CLIP-170 CAP-Gly domain 1
  2e4h		Solution structure of cytoskeletal protein in complex with tubulin tail
  2hkn		Crystal structure of the CAP-Gly domain of human Dynactin-1 (p150-Glued)
  2hkq		Crystal structure of the C-terminal domain of human EB1 in complex with the CAP-Gly domain of human Dynactin-1 (p150-Glued)
  2hl3		Crystal structure of the A49M mutant CAP-Gly domain of human Dynactin-1 (p150-Glued) in complex with human EB1 C-terminal hexapeptide
  2hl5		Crystal structure of the C-terminal domain of human EB1 in complex with the A49M mutant CAP-Gly domain of human Dynactin-1 (p150-Glued)
  2hqh		Crystal structure of p150Glued and CLIP-170
  2m02		3D structure of cap-gly domain of mammalian dynactin determined by magic angle spinning NMR spectroscopy
  2mpx		2MPX
  2qk0		Structural Basis of Microtubule Plus End Tracking by XMAP215, CLIP-170 and EB1
  2z0w		Crystal structure of the 2nd CAP-Gly domain in human Restin-like protein 2 reveals a swapped-dimer
  3e2u		Crystal structure of the zink-knuckle 2 domain of human CLIP-170 in complex with CAP-Gly domain of human dynactin-1 (p150-GLUED)
  3rdv		Structure of the SLAIN2c-CLIPCG1 complex
  3tq7		EB1c/EB3c heterodimer in complex with the CAP-Gly domain of P150glued
  4b6m		Trypansoma brucei tubulin binding cofactor B CAP-Gly domain

• Links (links to other resources describing this domain)

• INTERPRO	IPR000938
  PFAM	CAP_GLY

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