Letunic et al. (2017) Nucleic Acids Res doi: 10.1093/nar/gkx922
Letunic et al. (2020) Nucleic Acids Res doi: 10.1093/nar/gkaa937

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CTD Spt5 C-terminal nonapeptide repeat binding Spt4

SMART accession number:	SM01104
Description:	The C-terminal domain of the transcription elongation factor protein Spt5 is necessary for binding to Spt4 to form the functional complex that regulates early transcription elongation by RNA polymerase II. The complex may be involved in pre-mRNA processing through its association with mRNA capping enzymes. This CTD domain carries a regular nonapeptide repeat that can be present in up to 18 copies, as in S. pombe (PUBMED:19460865). The repeat has a characteristic TPA motif.
Interpro abstract (IPR024945):	The C-terminal domain of the transcription elongation factor protein Spt5 is necessary for binding to Spt4 to form the functional complex that regulates early transcription elongation by RNA polymerase II. The complex may be involved in pre-mRNA processing through its association with mRNA capping enzymes. This CTD domain carries a regular nonapeptide repeat that can be present in up to 18 copies, as in S. pombe [ (PUBMED:19460865) ]. The repeat has a characteristic TPA motif.
GO function:	protein binding (GO:0005515)
Family alignment:	View or CHROMA formatHMM/Web logoCLUSTALW formatMSF formatFASTA formatPIR format

There are 1111 CTD domains in 1071 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Taxonomic distribution of proteins containing CTD domain.

  This tree includes only several representative species. The complete taxonomic breakdown of all proteins with CTD domain is also avaliable.

  Click on the protein counts, or double click on taxonomic names to display all proteins containing CTD domain in the selected taxonomic class.

• Cellular role (predicted cellular role)

• Cellular role: transcription
  

• Literature (relevant references for this domain)

• Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.

  • Schwer B, Schneider S, Pei Y, Aronova A, Shuman S
  • Characterization of the Schizosaccharomyces pombe Spt5-Spt4 complex.
  • RNA. 2009; 15: 1241-50
  • Display abstract

  • The Spt5-Spt4 complex regulates early transcription elongation by RNApolymerase II and has an imputed role in pre-mRNA processing via itsphysical association with mRNA capping enzymes. Here we characterize theSchizosaccharomyces pombe core Spt5-Spt4 complex as a heterodimer and mapa trypsin-resistant Spt4-binding domain within the Spt5 subunit. A geneticanalysis of Spt4 in S. pombe revealed it to be inessential for growth at25 degrees C-30 degrees C but critical at 37 degrees C. These results echothe conditional spt4Delta growth phenotype in budding yeast, where we findthat Saccharomyces cerevisiae and S. pombe Spt4 are functionallyinterchangeable. Complementation of S. cerevisiae spt4Delta and atwo-hybrid assay for Spt4-Spt5 interaction provided a readout of theeffects of 33 missense and truncation mutations on S. pombe Spt4 functionin vivo, which were interpreted in light of the recent crystal structureof S. cerevisiae Spt4 fused to a fragment of Spt5. Our results highlightthe importance of the Spt4 Zn2+-binding residues--Cys12, Cys15, Cys29, andAsp32--and of Ser57, a conserved constituent of the Spt4-Spt5 interface.The 990-amino acid S. pombe Spt5 protein has an exceptionally regularcarboxyl-terminal domain (CTD) composed of 18 nonapeptide repeats. We findthat as few as three nonamer repeats sufficed for S. pombe growth, butonly when Spt4 was present. Synthetic lethality of the spt5(1-835)spt4Delta double mutant at 34 degrees C suggests that interaction of Spt4with the central domain of Spt5 overlaps functionally with the Spt5 CTD.

• Links (links to other resources describing this domain)

• PFAM	CTD
  INTERPRO	IPR024945

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