C2

Protein kinase C conserved region 2 (CalB)
C2
SMART accession number:SM00239
Description: Ca2+-binding motif present in phospholipases, protein kinases C, and synaptotagmins (among others). Some do not appear to contain Ca2+-binding sites. Particular C2s appear to bind phospholipids, inositol polyphosphates, and intracellular proteins. Unusual occurrence in perforin. Synaptotagmin and PLC C2s are permuted in sequence with respect to N- and C-terminal beta strands. SMART detects C2 domains using one or both of two profiles.
Interpro abstract (IPR000008): The C2 domain is a Ca2+-dependent membrane-targeting module found in many cellular proteins involved in signal transduction or membrane trafficking. C2 domains are unique among membrane targeting domains in that they show wide range of lipid selectivity for the major components of cell membranes, including phosphatidylserine and phosphatidylcholine. This C2 domain is about 116 amino-acid residues and is located between the two copies of the C1 domain in Protein Kinase C (that bind phorbol esters and diacylglycerol) and the protein kinase catalytic domain . Regions with significant homology [(PUBMED:7559667)] to the C2-domain have been found in many proteins. The C2 domain is thought to be involved in calcium-dependent phospholipid binding [(PUBMED:8253763)] and in membrane targetting processes such as subcellular localisation.

The 3D structure of the C2 domain of synaptotagmin has been reported [(PUBMED:7697723)], the domain forms an eight-stranded beta sandwich constructed around a conserved 4-stranded motif, designated a C2 key [(PUBMED:7697723)]. Calcium binds in a cup-shaped depression formed by the N- and C-terminal loops of the C2-key motif. Structural analyses of several C2 domains have shown them to consist of similar ternary structures in which three Ca2+-binding loops are located at the end of an 8 stranded antiparallel beta sandwich.

GO function:protein binding (GO:0005515)
Family alignment:
View or

There are 47478 C2 domains in 29474 proteins in SMART's nrdb database.

Click on the following links for more information.