Schultz et al. (1998) Proc. Natl. Acad. Sci. USA 95, 5857-5864
Letunic et al. (2012) Nucleic Acids Res , doi:10.1093/nar/gkr931

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EXOIII

SMART accession number:	SM00479
Description:	exonuclease domain in DNA-polymerase alpha and epsilon chain, ribonuclease T and other exonucleases
Interpro abstract (IPR006055):	This entry includes a variety of exonuclease proteins, such as ribonuclease T [(PUBMED:8506149)] and the epsilon subunit of DNA polymerase III. Ribonuclease T is responsible for the end-turnover of tRNA,and removes the terminal AMP residue from uncharged tRNA. DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria, and also exhibits 3' to 5' exonuclease activity.
GO component:	intracellular (GO:0005622)
GO function:	exonuclease activity (GO:0004527)
Family alignment:	View or CHROMA formatCLUSTALW formatMSF formatFASTA formatPIR format

There are 5444 EXOIII domains in 5443 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Click on to expand nodes. To display all proteins with a EXOIII domain in a specific node, click on it.

  This tree shows only several representative species. The complete taxonomic breakdown of all proteins with EXOIII domain is also avaliable.

  Useful shortcuts: Expand all nodes or Collapse tree
  Go to specific node: Anopheles gambiae, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae, Takifugu rubripes

• Cellular role (predicted cellular role)

• Cellular role: replication
  

• Literature (relevant references for this domain)

• Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.

  • Koonin EV, Deutscher MP
  • RNase T shares conserved sequence motifs with DNA proofreading exonucleases.
  • Nucleic Acids Res. 1993; 21: 2521-2
• Metabolism (metabolic pathways involving proteins which contain this domain)

• Click the image to view the interactive version of the map in iPath

  % proteins involved KEGG pathway ID Description 99.10 map03030 DNA replication 0.45 map00240 Pyrimidine metabolism 0.45 map00230 Purine metabolism This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with EXOIII domain which could be assigned to a KEGG orthologous group, and not all proteins containing EXOIII domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

• Structure (3D structures containing this domain)

• 3D Structures of EXOIII domains in PDB

  PDB code	Main view	Title
  1j53		Structure of the n-terminal exonuclease domain of the epsilon subunit of e.coli dna polymerase iii at ph 8.5
  1j54		Structure of the n-terminal exonuclease domain of the epsilon subunit of e.coli dna polymerase iii at ph 5.8
  1j9a		Oligoribonuclease
  1w0h		Crystallographic structure of the nuclease domain of 3'hexo, a deddh family member, bound to ramp
  1wlj		Human isg20
  1y97		The human trex2 3' exonuclease structure suggests a mechanism for efficient non-processive dna catalysis
  1yta		Crystal structure of oligoribonuclease, the lone essential exoribonuclease in escherichia coli
  1zbh		3'-end specific recognition of histone mrna stem-loop by 3'- exonuclease
  1zbu		Crystal structure of full-length 3'-exonuclease
  2f96		2.1 a crystal structure of pseudomonas aeruginosa rnase t (ribonuclease t)
  2gbz		The crystal structure of xc847 from xanthomonas campestris: a 3-5 oligoribonuclease of dnaq fold family with a novel opposingly-shifted helix
  2gui		Structure and funciton of cyclized versions of the proofreading exonuclease subunit of e. coli dna polymerase iii
  2ido		Structure of the e. coli pol iii epsilon-hot proofreading complex
  2igi		Crystal structure of e. coli oligoribonuclease
  2ioc		The crystal structure of trex1 explains the 3' nucleotide specificity and reveals a polyproline ii helix for protein partenring
  2is3		Crystal structure of escherichia coli rnase t
  2o4g		Structure of trex1 in complex with a nucleotide
  2o4i		Structure of trex1 in complex with dna
  2oa8		Crystal structure of mtrex1 with ssdna
  2p1j		Crystal structure of a polc-type dna polymerase iii exonuclease domain from thermotoga maritima
  3b6o		Structure of trex1 in complex with a nucleotide and an inhibitor ion (lithium)
  3b6p		Structure of trex1 in complex with a nucleotide and inhibitor ions (sodium and zinc)
  3cg7		Crystal structure of cell-death related nuclease 4 (crn-4)
  3cm5		Crystal structure of cell-death related nuclease 4 (crn-4) bound with mn
  3cm6		Crystal structure of cell-death related nuclease 4 (crn-4) bound with er

• Links (links to other resources describing this domain)

• PFAM	Exonuclease
  INTERPRO	IPR006055

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