Schultz et al. (1998) Proc. Natl. Acad. Sci. USA 95, 5857-5864
Letunic et al. (2012) Nucleic Acids Res , doi:10.1093/nar/gkr931

• HOME
• SETUP
• FAQ
• ABOUT
• GLOSSARY
• WHAT'S NEW
• FEEDBACK

Glyco_32 Glycosyl hydrolases family 32

SMART accession number:	SM00640
Description:
Interpro abstract (IPR001362):	O-Glycosyl hydrolases (EC 3.2.1.) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [(PUBMED:7624375), (PUBMED:8535779)]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site. Glycoside hydrolase family 32 comprises enzymes with several known activities; invertase (EC 3.2.1.26); inulinase (EC 3.2.1.7); levanase (EC 3.2.1.65); exo-inulinase (EC 3.2.1.80); sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99); fructan:fructan 1-fructosyltransferase (EC 2.4.1.100).
GO process:	carbohydrate metabolic process (GO:0005975)
GO function:	hydrolase activity, hydrolyzing O-glycosyl compounds (GO:0004553)
Family alignment:	View or CHROMA formatCLUSTALW formatMSF formatFASTA formatPIR format

There are 1554 Glyco_32 domains in 1552 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Click on to expand nodes. To display all proteins with a Glyco_32 domain in a specific node, click on it.

  This tree shows only several representative species. The complete taxonomic breakdown of all proteins with Glyco_32 domain is also avaliable.

  Useful shortcuts: Expand all nodes or Collapse tree
  Go to specific node: Arabidopsis thaliana, Saccharomyces cerevisiae

• Metabolism (metabolic pathways involving proteins which contain this domain)

• Click the image to view the interactive version of the map in iPath

  % proteins involved KEGG pathway ID Description 51.37 map00500 Starch and sucrose metabolism 45.49 map00052 Galactose metabolism 2.35 map00530 Aminosugars metabolism 0.78 map00051 Fructose and mannose metabolism This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with Glyco_32 domain which could be assigned to a KEGG orthologous group, and not all proteins containing Glyco_32 domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

• Structure (3D structures containing this domain)

• 3D Structures of Glyco_32 domains in PDB

  PDB code	Main view	Title
  1st8		Crystal structure of fructan 1-exohydrolase iia from cichorium intybus
  1uyp		The three-dimensional structure of beta-fructosidase (invertase) from thermotoga maritima
  1w2t		Beta-fructosidase from thermotoga maritima in complex with raffinose
  1y4w		Crystal structure of exo-inulinase from aspergillus awamori in spacegroup p21
  1y9g		Crystal structure of exo-inulinase from aspergillus awamori complexed with fructose
  1y9m		Crystal structure of exo-inulinase from aspergillus awamori in spacegroup p212121
  2ac1		Crystal structure of a cell-wall invertase from arabidopsis thaliana
  2add		Crystal structure of fructan 1-exohydrolase iia from cichorium intybus in complex with sucrose
  2ade		Crystal structure of fructan 1-exohydrolase iia from cichorium intybus in complex with fructose
  2aey		Crystal structure of fructan 1-exohydrolase iia from cichorium intybus in complex with 2,5 dideoxy-2,5-immino-d- mannitol
  2aez		Crystal structure of fructan 1-exohydrolase iia (e201q) from cichorium intybus in complex with 1-kestose
  2oxb		Crystal structure of a cell-wall invertase (e203q) from arabidopsis thaliana in complex with sucrose
  2qqu		Crystal structure of a cell-wall invertase (d239a) from arabidopsis thaliana in complex with sucrose
  2qqv		Crystal structure of a cell-wall invertase (e203a) from arabidopsis thaliana in complex with sucrose
  2qqw		Crystal structure of a cell-wall invertase (d23a) from arabidopsis thaliana in complex with sucrose

• Links (links to other resources describing this domain)

• PFAM	Glyco_hydro_32
  INTERPRO	IPR001362

Send comments to Ivica Letunic