The virD operon in Agrobacterium encodes a site-specific endonuclease, and a number of other poorly characterised products. This family represents the VirD5 protein.
The virD operon in Agrobacterium encodes a site-specific endonuclease, and a number of other poorly characterised products [ (PUBMED:3658701) ]. This family represents the VirD5 protein.
Family alignment:
There are 14 Agro_virD5 domains in 14 proteins in SMART's nrdb database.
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Evolution (species in which this domain is found)
Taxonomic distribution of proteins containing Agro_virD5 domain.
This tree includes only several representative species. The complete taxonomic breakdown of all proteins with Agro_virD5 domain is also avaliable.
Click on the protein counts, or double click on taxonomic names to display all proteins containing Agro_virD5 domain in the selected taxonomic class.
Molecular characterization of the virD operon from Agrobacterium tumefaciens.
Nucleic Acids Res. 1987; 15: 7503-17
Display abstract
The Agrobacterium tumefaciens Ti plasmid virulence (vir) region contains at least six transcriptional units required for the efficient transfer of T-DNA to the plant genome (virA, B, C, D, E, and G). We have reported that two proteins encoded by the 5'portion of the virD operon are required for a site-specific endonuclease activity that nicks the direct repeats which flank the T-DNA. We have presented the nucleotide sequence for this portion of the operon. The nucleotide sequence of the remainder of the virD operon essential for virulence has now been determined. Two additional open reading frames encode proteins of 21.3 and 75.8 kilodaltons (kd). Translational fusions between virD2, virD3, and virD4 proteins and trpE produced fusion proteins of the size predicted from the nucleotide sequence data. We have used antisera directed against the trpE-virD2 fusion protein to detect both native virD2 protein and a virD2-lacZ fusion protein in crude extracts from Agrobacterium.
Links (links to other resources describing this domain)
