Schultz et al. (1998) Proc. Natl. Acad. Sci. USA 95, 5857-5864
Letunic et al. (2012) Nucleic Acids Res , doi:10.1093/nar/gkr931

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Alpha-mann_mid Alpha mannosidase, middle domain

SMART accession number:	SM00872
Description:	Members of this entry belong to the glycosyl hydrolase family 38, This domain, which is found in the central region adopts a structure consisting of three alpha helices, in an immunoglobulin/albumin-binding domain-like fold. The domain is predominantly found in the enzyme alpha-mannosidase (PUBMED:12634058).
Interpro abstract (IPR015341):	Members of this entry belong to the glycosyl hydrolase family 38, This domain, which is found in the central region adopts a structure consisting of three alpha helices, in an immunoglobulin/albumin-binding domain-like fold. The domain is predominantly found in the enzyme alpha-mannosidase [(PUBMED:12634058)].
GO function:	hydrolase activity, hydrolyzing O-glycosyl compounds (GO:0004553), zinc ion binding (GO:0008270)
Family alignment:	View or CHROMA formatCLUSTALW formatMSF formatFASTA formatPIR format

There are 1113 Alpha-mann_mid domains in 1112 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Click on to expand nodes. To display all proteins with a Alpha-mann_mid domain in a specific node, click on it.

  This tree shows only several representative species. The complete taxonomic breakdown of all proteins with Alpha-mann_mid domain is also avaliable.

  Useful shortcuts: Expand all nodes or Collapse tree
  Go to specific node: Anopheles gambiae, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae, Takifugu rubripes

• Cellular role (predicted cellular role)

• Cellular role: metabolism
  

• Metabolism (metabolic pathways involving proteins which contain this domain)

• Click the image to view the interactive version of the map in iPath

  % proteins involved KEGG pathway ID Description 43.57 map00511 N-Glycan degradation 43.57 map01032 Glycan structures - degradation 5.81 map00510 N-Glycan biosynthesis 5.81 map01030 Glycan structures - biosynthesis 1 1.24 map00530 Aminosugars metabolism This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with Alpha-mann_mid domain which could be assigned to a KEGG orthologous group, and not all proteins containing Alpha-mann_mid domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

• Structure (3D structures containing this domain)

• 3D Structures of Alpha-mann_mid domains in PDB

  PDB code	Main view	Title
  1hty		Golgi alpha-mannosidase ii
  1hww		Golgi alpha-mannosidase ii in complex with swainsonine
  1hxk		Golgi alpha-mannosidase ii in complex with deoxymannojirimicin
  1ps3		Golgi alpha-mannosidase ii in complex with kifunensine
  1qwn		Golgi alpha-mannosidase ii covalent intermediate complex with 5-fluoro-gulosyl-fluoride
  1qwu		Golgi alpha-mannosidase ii d341n mutant complex with 5-f- guloside
  1qx1		Golgi alpha-mannosidase ii d341n mutant complex with 2-f- mannosyl-f
  1r33		Golgi alpha-mannosidase ii complex with 5-thio-d- mannopyranosylamine
  1r34		Golgi alpha-mannosidase ii complex with 5-thio-d- mannopyranosylamidinium salt
  1tqs		Golgi alpha-mannosidase ii in complex with salacinol
  1tqt		Golgi alpha-mannosidase ii in complex with a diastereomer of salacinol
  1tqu		Golgi alpha-mannosidase ii in complex with the salacinol analog ghavamiol
  1tqv		Golgi alpha-mannosidase ii in complex with seleno-salacinol (blintol)
  1tqw		Golgi alpha-mannosidase ii in complex with a diastereomer of seleno-salacinol
  2alw		Golgi alpha-mannosidase ii complex with noeuromycin
  2f18		Golgi alpha-mannosidase ii complex with (2r,3r,4s)-2- ({[(1r)-2-hydroxy-1-phenylethyl]amino}methyl)pyrrolidine-3, 4-diol
  2f1a		Golgi alpha-mannosidase ii complex with (2r,3r,4s)-2- ({[(1s)-2-hydroxy-1-phenylethyl]amino}methyl)pyrrolidine-3, 4-diol
  2f1b		Golgi alpha-mannosidase ii complex with (2r,3r,4s,5r)-2- ({[(1r)-2-hydroxy-1-phenylethyl]amino}methyl)-5- methylpyrrolidine-3,4-diol
  2f7o		Golgi alpha-mannosidase ii complex with mannostatin a
  2f7p		Golgi alpha-mannosidase ii complex with benzyl-mannostatin a
  2f7q		Golgi alpha-mannosidase ii complex with aminocyclopentitetrol
  2f7r		Golgi alpha-mannosidase ii complex with benzyl- aminocyclopentitetrol
  2fyv		Golgi alpha-mannosidase ii complex with an amino-salacinol carboxylate analog
  2ow6		Golgi alpha-mannosidase ii complex with (1r,5s,6s,7r,8s)-1- thioniabicyclo[4.3.0]nonan-5,7,8-triol chloride
  2ow7		Golgi alpha-mannosidase ii complex with (1r,6s,7r,8s)-1- thioniabicyclo[4.3.0]nonan-7,8-diol chloride
  3blb		Crystal structure of golgi mannosidase ii in complex with swainsonine at 1.3 angstrom resolution
  3bub		Golgi alpha-mannosidase ii with an empty active site
  3bud		Golgi mannosidase ii d204a catalytic nucleophile mutant with an empty active site
  3bui		Golgi mannosidase ii d204a catalytic nucleophile mutant complex with tris
  3bup		Golgi alpha-mannosidase ii d341n acid-base catalyst mutant with bound mannose
  3buq		Golgi alpha-mannosidase ii d204a catalytic nucleophile mutant with bound mannose.
  3bvt		Golgi mannosidase ii d204a catalytic nucleophile mutant complex with methyl (alpha-d-mannopyranosyl)-(1->3)-s- alpha-d-mannopyranoside
  3bvu		Golgi mannosidase ii d204a catalytic nucleophile mutant complex with methyl(alpha-d-mannopyranosyl)-(1->3)-s- [(alpha-d-mannopyranosyl)-(1->6)]-alpha-d-mannopyranoside
  3bvv		Golgi mannosidase ii d204a catalytic nucleophile mutant complex with methyl alpha-d-mannopyranosyl-(1->3)-[6-thio- alpha-d-mannopyranosyl-(1->6)]-beta-d-mannopyranoside
  3bvw		Golgi mannosidase ii d204a catalytic nucleophile mutant complex with methyl (2-deoxy-2-acetamido-beta-d- glucopyranosyl)-(1->2)-alpha-d-mannopyranosyl- (1->3)- [alpha-d-mannopyranosyl-(1->6)-6-thio-alpha-d- mannopyranosyl- (1->6)]-beta-d-mannopyranoside
  3bvx		Golgi mannosidase ii d204a catalytic nucleophile mutant complex with methyl (2-deoxy-2-acetamido-beta-d- glucopyranosyl)-(1->2)-(alpha-d-mannopyranosyl)- (1->3)- [(alpha-d-mannopyranosyl)-(1->6)-(alpha-d-mannopyranosyl)- (1->6)]-beta-d-mannopyranoside
  3cv5		Golgi mannosidase ii d204a catalytic nucleophile mutant complex with 3alpha,6alpha-mannopentaose
  3czn		Golgi alpha-mannosidase ii (d204a nucleophile mutant) in complex with gnman5gn
  3czs		Golgi alpha-mannosidase ii (d204a nucleophile mutant)
  3d4y		Golgi mannosidase ii complex with mannoimidazole
  3d4z		Golgi mannosidase ii complex with gluco-imidazole
  3d50		Golgi mannosidase ii complex with n-octyl-6-epi-valienamine
  3d51		Golgi mannosidase ii complex with gluco-hydroxyiminolactam
  3d52		Golgi mannosidase ii complex with an n-aryl carbamate derivative of gluco-hydroxyiminolactam
  3ddf		Golgi mannosidase ii complex with (3r,4r,5r)-3,4-dihydroxy- 5-({[(1r)-2-hydroxy-1 phenylethyl]amino}methyl) pyrrolidin- 2-one
  3ddg		Golgi mannosidase ii complex with (3r,4r,5r)-3,4-dihydroxy- 5-({[(1r)-2-hydroxy-1 phenylethyl]amino}methyl) methylpyrrolidin-2-one
  3dx0		Golgi alpha-mannosidase ii in complex with mannostatin a at ph 5.75
  3dx1		Golgi alpha-mannosidase ii in complex with mannostatin analog (1s,2s,3r,4r)-4-aminocyclopentane-1,2,3-triol
  3dx2		Golgi mannosidase ii complex with mannostatin b
  3dx3		Golgi alpha-mannosidase ii in complex with mannostatin analog (1r,2r,3s,4r,5r)-5-aminocyclopentane-1,2,3,4-tetraol
  3dx4		Golgi alpha-mannosidase ii in complex with mannostatin analog (1r,2r,3r,4s,5r)-4-amino-5-methoxycyclopentane-1,2, 3-triol
  3ejp		Golgi alpha-mannosidase ii in complex with 5-substituted swainsonine analog: (5r)-5-[2'-oxo-2'-(phenyl)ethyl]- swainsonine
  3ejq		Golgi alpha-mannosidase ii in complex with 5-substitued swainsonine analog: (5r)-5-[2'-oxo-2'-(4-methylphenyl) ethyl]-swainsonine
  3ejr		Golgi alpha-mannosidase ii in complex with 5-substitued swainsonine analog: (5r)-5-[2'-oxo-2'-(4-tert-butylphenyl) ethyl]-swainsonine
  3ejs		Golgi alpha-mannosidase ii in complex with 5-substituted swainsonine analog: (5s)-5-[2'-(4-tert-butylphenyl)ethyl]- swainsonine
  3ejt		Golgi alpha-mannosidase ii in complex with 5-substituted swainsonine analog:(5r)-5-[2'-(4-tert-butylphenyl)ethyl]- swainsonine
  3eju		Golgi alpha-mannosidase ii in complex with 5-substituted swainsonine analog:(5s)-5-[2'-oxo-2'-(4-tert-butylphenyl) ethyl]-swainsonine

• Links (links to other resources describing this domain)

• PFAM	Alpha-mann_mid
  INTERPRO	IPR015341

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