Schultz et al. (1998) Proc. Natl. Acad. Sci. USA 95, 5857-5864
Letunic et al. (2012) Nucleic Acids Res , doi:10.1093/nar/gkr931

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BAH Bromo adjacent homology domain

SMART accession number:	SM00439
Description:
Interpro abstract (IPR001025):	The BAH (bromo-adjacent homology) family contains proteins such as eukaryotic DNA (cytosine-5) methyltransferases IPR001525, the origin recognition complex 1 (Orc1) proteins, as well as several proteins involved in transcriptional regulation. The BAH domain appears to act as a protein-protein interaction module specialised in gene silencing, as suggested for example by its interaction within yeast Orc1p with the silent information regulator Sir1p. The BAH module might therefore play an important role by linking DNA methylation, replication and transcriptional regulation [(PUBMED:10100640)].
GO function:	DNA binding (GO:0003677)
Family alignment:	View or CHROMA formatCLUSTALW formatMSF formatFASTA formatPIR format

There are 1082 BAH domains in 903 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Click on to expand nodes. To display all proteins with a BAH domain in a specific node, click on it.

  This tree shows only several representative species. The complete taxonomic breakdown of all proteins with BAH domain is also avaliable.

  Useful shortcuts: Expand all nodes or Collapse tree
  Go to specific node: Anopheles gambiae, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae, Takifugu rubripes

• Literature (relevant references for this domain)

• Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.

  • Callebaut I, Courvalin JC, Mornon JP
  • The BAH (bromo-adjacent homology) domain: a link between DNA methylation, replication and transcriptional regulation.
  • FEBS Lett. 1999; 446: 189-93
  • Display abstract

  • Using sensitive methods of sequence analysis including hydrophobic cluster analysis, we report here a hitherto undescribed family of modules, the BAH (bromo-adjacent homology) family, which includes proteins such as eukaryotic DNA (cytosine-5) methyltransferases, the origin recognition complex 1 (Orc1) proteins, as well as several proteins involved in transcriptional regulation. The BAH domain appears to act as a protein-protein interaction module specialized in gene silencing, as suggested for example by its interaction within yeast Orc1p with the silent information regulator Sir1p. The BAH module might therefore play an important role by linking DNA methylation, replication and transcriptional regulation.

  • Nicolas RH, Goodwin GH
  • Molecular cloning of polybromo, a nuclear protein containing multiple domains including five bromodomains, a truncated HMG-box, and two repeats of a novel domain.
  • Gene. 1996; 175: 233-40
  • Display abstract

  • A number of transcription factors that act as adaptor proteins have been found to contain an 87 amino acid domain called the bromodomain. In a study to identify and characterise bromodomain proteins expressed in chicken cells, a novel gene has been isolated which encodes five repeats of the bromodomain. In addition, the encoded protein, termed polybromo, contains four other domains: an unusual truncated HMG box, two repeats of a novel domain which we term the BAH domain and a sequence related to a region within the regulatory domain of the DNA cytosine-5 methyltransferase enzyme. Polybromo was found to be related to a yeast protein U19102 which has two bromo domains, a BAH domain and the DNA methyltransferase-related sequence. Antibodies that were raised against polybromo were used in confocal microscopy analysis to show that the 180-kDa polybromo protein is located within the nucleus but excluded from the nucleolus. Gel filtration analysis of nuclear extracts demonstrate that polybromo is part of a large complex with a mass of approximately 2 million dalton.

• Metabolism (metabolic pathways involving proteins which contain this domain)

• Click the image to view the interactive version of the map in iPath

  % proteins involved KEGG pathway ID Description 28.89 map04111 Cell cycle - yeast 28.89 map04110 Cell cycle 22.22 map00271 Methionine metabolism 13.33 map04530 Tight junction 6.67 map05050 Dentatorubropallidoluysian atrophy (DRPLA) This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with BAH domain which could be assigned to a KEGG orthologous group, and not all proteins containing BAH domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

• Structure (3D structures containing this domain)

• 3D Structures of BAH domains in PDB

  PDB code	Main view	Title
  1m4z		Crystal structure of the n-terminal bah domain of orc1p
  1w4s		Crystal structure of the proximal bah domain of polybromo
  1zbx		Crystal structure of a orc1p-sir1p complex
  1zhi		Complex of the s. cerevisiae orc1 and sir1 interacting domains
  2fl7		S. cerevisiae sir3 bah domain
  2fvu		Structure of the yeast sir3 bah domain

• Links (links to other resources describing this domain)

• PFAM	BAH
  INTERPRO	IPR001025

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