BHD_2Rad4 beta-hairpin domain 2 |
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| SMART accession number: | SM01031
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| Description: |
This short domain is found in the Rad4 protein. This domain binds to DNA. |
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| Interpro abstract (IPR018327): |
This short domain is found in the Rad4 protein. This domain binds to DNA [(PUBMED:17882165)].
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| GO function: | DNA binding (GO:0003677) |
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| Family alignment: |
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There are 217
BHD_2 domains in 217 proteins in SMART's nrdb database.
Click on the following links for more information.
- Evolution (species in which this domain is found)
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- Cellular role (predicted cellular role)
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Cellular role: chromatin
- Literature (relevant references for this domain)
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Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.
- Min JH, Pavletich NP
- Recognition of DNA damage by the Rad4 nucleotide excision repair protein.
- Nature. 2007; 449: 570-5
- Display abstract
Mutations in the nucleotide excision repair (NER) pathway can cause thexeroderma pigmentosum skin cancer predisposition syndrome. NER lesions arelimited to one DNA strand, but otherwise they are chemically andstructurally diverse, being caused by a wide variety of genotoxicchemicals and ultraviolet radiation. The xeroderma pigmentosum C (XPC)protein has a central role in initiating global-genome NER by recognizingthe lesion and recruiting downstream factors. Here we present the crystalstructure of the yeast XPC orthologue Rad4 bound to DNA containing acyclobutane pyrimidine dimer (CPD) lesion. The structure shows that Rad4inserts a beta-hairpin through the DNA duplex, causing the two damagedbase pairs to flip out of the double helix. The expelled nucleotides ofthe undamaged strand are recognized by Rad4, whereas the two CPD-linkednucleotides become disordered. These findings indicate that the lesionsrecognized by Rad4/XPC thermodynamically destabilize the Watson-Crickdouble helix in a manner that facilitates the flipping-out of two basepairs.
- Structure (3D structures containing this domain)
3D Structures of BHD_2 domains in PDB
| PDB code | Main view | Title | | 2qsf |  | Crystal structure of the rad4-rad23 complex |
| 2qsg |  | Crystal structure of rad4-rad23 bound to a uv-damaged dna |
| 2qsh |  | Crystal structure of rad4-rad23 bound to a mismatch dna |
- Links (links to other resources describing this domain)
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to expand nodes. To display all proteins with a BHD_2 domain in a specific node, click on it.