CDC48_2Cell division protein 48 (CDC48) domain 2 |
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| SMART accession number: | SM01072
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| Description: |
This domain has a double psi-beta barrel fold and includes VCP-like ATPase and N-ethylmaleimide sensitive fusion protein N-terminal domains. Both the VAT and NSF N-terminal functional domains consist of two structural domains of which this is at the C-terminus. The VAT-N domain found in AAA ATPases is a substrate 185-residue recognition domain (PUBMED:10531028). |
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| Interpro abstract (IPR004201): |
The CDC48 N-terminal domain is a protein domain found in AAA ATPases including cell division protein 48 (CDC48), VCP-like ATPase (VAT) and N-ethylmaleimide sensitive fusion protein. It is a substrate recognition domain which binds polypeptides, prevents protein aggregation, and catalyses refolding of permissive substrates. It is composed of two equally sized subdomains. The amino-terminal subdomain forms a double-psi beta-barrel whose pseudo-twofold symmetry is mirrored by an internal sequence repeat of 42 residues. The carboxy-terminal subdomain forms a novel six-stranded beta-clam fold [(PUBMED:10531028)]. Together these subdomains form a kidney-shaped structure. This entry represents the carboxy-terminal subdomain.
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| Family alignment: |
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There are 541
CDC48_2 domains in 541 proteins in SMART's nrdb database.
Click on the following links for more information.
- Evolution (species in which this domain is found)
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- Cellular role (predicted cellular role)
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Cellular role: metabolism
- Literature (relevant references for this domain)
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Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.
- Coles M et al.
- The solution structure of VAT-N reveals a 'missing link' in the evolutionof complex enzymes from a simple betaalphabetabeta element.
- Curr Biol. 1999; 9: 1158-68
- Display abstract
BACKGROUND: The VAT protein of the archaebacterium Thermoplasmaacidophilum, like all other members of the Cdc48/p97 family of AAAATPases, has two ATPase domains and a 185-residue amino-terminalsubstrate-recognition domain, VAT-N. VAT shows activity in protein foldingand unfolding and thus shares the common function of these ATPases indisassembly and/or degradation of protein complexes. RESULTS: Usingnuclear magnetic resonance (NMR) spectroscopy, we found that VAT-N iscomposed of two equally sized subdomains. The amino-terminal subdomainVAT-Nn (comprising residues Met1-Thr92) forms a double-psi beta-barrelwhose pseudo-twofold symmetry is mirrored by an internal sequence repeatof 42 residues. The carboxy-terminal subdomain VAT-Nc (comprising residuesGlu93-Gly185) forms a novel six-stranded beta-clam fold. Together, VAT-Nnand VAT-Nc form a kidney-shaped structure, in close agreement with resultsfrom electron microscopy. Sequence and structure analyses showed thatVAT-Nn is related to numerous proteins including prokaryotic transcriptionfactors, metabolic enzymes, the protease cofactors UFD1 and PrlF, andaspartic proteinases. These proteins map out an evolutionary path fromsimple homodimeric transcription factors containing a single copy of theVAT-Nn repeat to complex enzymes containing four copies. CONCLUSIONS: Ourresults suggest that VAT-N is a precursor of the aspartic proteinases thathas acquired peptide-binding activity while remaining proteolyticallyincompetent. We propose that the binding site of the protein is similar tothat of aspartic proteinases, in that it lies between the psi-loops of theamino-terminal beta-barrel and that it coincides with a crescent-shapedband of positive charge extending across the upper face of the molecule.
- Structure (3D structures containing this domain)
3D Structures of CDC48_2 domains in PDB
| PDB code | Main view | Title | | 1cr5 |  | N-terminal domain of sec18p |
| 1cz4 |  | Nmr structure of vat-n: the n-terminal domain of vat (vcp- like atpase of thermoplasma) |
| 1cz5 |  | Nmr structure of vat-n: the n-terminal domain of vat (vcp- like atpase of thermoplasma) |
| 1e32 |  | Structure of the n-terminal domain and the d1 aaa domain of membrane fusion atpase p97 |
| 1qcs |  | N-terminal domain of n-ethylmaleimide sensitive factor (nsf) |
| 1qdn |  | Amino terminal domain of the n-ethylmaleimide sensitive fusion protein (nsf) |
| 1r7r |  | The crystal structure of murine p97/vcp at 3.6a |
| 1s3s |  | Crystal structure of aaa atpase p97/vcp nd1 in complex with p47 c |
| 2jv2 |  | Solution structure of the n-terminal domain of ph1500 |
| 2pjh |  | Strctural model of the p97 n domain- npl4 ubd complex |
| 3cf1 |  | Structure of p97/vcp in complex with adp/adp.alfx |
| 3cf2 |  | Structure of p97/vcp in complex with adp/amp-pnp |
| 3cf3 |  | Structure of p97/vcp in complex with adp |
- Links (links to other resources describing this domain)
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