SMART: Cdc6_C domain annotation

Cdc6_C CDC6, C terminal

SMART accession number:	SM01074
Description:	The C terminal domain of CDC6 assumes a winged helix fold, with a five alpha-helical bundle (alpha15-alpha19) structure, backed on one side by three beta strands (beta6-beta8). It has been shown that this domain acts as a DNA-localisation factor, however its exact function is, as yet, unknown. Putative functions include: (1) mediation of protein-protein interactions and (2) regulation of nucleotide binding and hydrolysis. Mutagenesis studies have shown that this domain is essential for appropriate Cdc6 activity (PUBMED:11030343).
Interpro abstract (IPR015163):	The C-terminal domain of cell division control protein 6 (CDC6) assumes a winged helix fold, with a five alpha-helical bundle (alpha15-alpha19) structure, backed on one side by three beta strands (beta6-beta8). It has been shown that this domain acts as a DNA-localisation factor, however its exact function is, as yet, unknown. Putative functions include: (1) mediation of protein-protein interactions and (2) regulation of nucleotide binding and hydrolysis. Mutagenesis studies have shown that this domain is essential for appropriate CDC6 activity [(PUBMED:11030343)].
Family alignment:	View or

There are 352 Cdc6_C domains in 352 proteins in SMART's nrdb database.

Click on the following links for more information.

Evolution (species in which this domain is found)
Click on to expand nodes. To display all proteins with a Cdc6_C domain in a specific node, click on it.

This tree shows only several representative species. The complete taxonomic breakdown of all proteins with Cdc6_C domain is also avaliable.

Useful shortcuts: Expand all nodes or Collapse tree
Go to specific node: Anopheles gambiae, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens, Mus musculus, Rattus norvegicus, Takifugu rubripes
Cellular role (predicted cellular role)
Cellular role: chromatin
Literature (relevant references for this domain)
Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.
- Liu J, Smith CL, DeRyckere D, DeAngelis K, Martin GS, Berger JM
- Structure and function of Cdc6/Cdc18: implications for origin recognitionand checkpoint control.
- Mol Cell. 2000; 6: 637-48
- Display abstract
- Cdc6/Cdc18 is a conserved and essential component of prereplicationcomplexes. The 2.0 A crystal structure of an archaeal Cdc6 ortholog, inconjunction with a mutational analysis of the homologous Cdc18 proteinfrom Schizosaccharomyces pombe, reveals novel aspects of Cdc6/Cdc18function. Two domains of Cdc6 form an AAA+-type nucleotide binding foldthat is observed bound to Mg.ADP. A third domain adopts a winged-helixfold similar to known DNA binding modules. Sequence comparisons show thatthe winged-helix domain is conserved in Orc1, and mutagenesis datademonstrate that this region of Cdc6/Cdc18 is required for function invivo. Additional mutational analyses suggest that nucleotide bindingand/or hydrolysis by Cdc6/Cdc18 is required not only for progressionthrough S phase, but also for maintenance of checkpoint control during Sphase.
Structure (3D structures containing this domain)

Links (links to other resources describing this domain)
PFAM Cdc6_C
INTERPRO IPR015163

PDB code	Main view	Title
1fnn		Crystal structure of cdc6p from pyrobaculum aerophilum
1w5s		Structure of the aeropyrum pernix orc2 protein (adp form)
1w5t		Structure of the aeropyrum pernix orc2 protein (adpnp-adp complexes)
2qby		Crystal structure of a heterodimer of cdc6/orc1 initiators bound to origin dna (from s. solfataricus)
2v1u		Structure of the aeropyrum pernix orc1 protein in complex with dna

PFAM	Cdc6_C
INTERPRO	IPR015163

Cdc6_C

3D Structures of Cdc6_C domains in PDB