Letunic et al. (2017) Nucleic Acids Res doi: 10.1093/nar/gkx922
Letunic et al. (2020) Nucleic Acids Res doi: 10.1093/nar/gkaa937

• HOME
• SETUP
• FAQ
• ABOUT
• GLOSSARY
• WHAT'S NEW
• FEEDBACK

Glyco_32 Glycosyl hydrolases family 32

SMART accession number:	SM00640
Description:	-
Interpro abstract (IPR001362):	O-Glycosyl hydrolases ( EC 3.2.1. ) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [ (PUBMED:7624375) (PUBMED:8535779) ]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website. Glycoside hydrolase family 32 comprises enzymes with several known activities; invertase/fructofuranosidase ( EC 3.2.1.26 ); inulinase ( EC 3.2.1.7 ); levanase ( EC 3.2.1.65 ); exo-inulinase ( EC 3.2.1.80 ); sucrose:sucrose 1-fructosyltransferase ( EC 2.4.1.99 ); fructan:fructan 1-fructosyltransferase ( EC 2.4.1.100 ).
GO process:	carbohydrate metabolic process (GO:0005975)
GO function:	hydrolase activity, hydrolyzing O-glycosyl compounds (GO:0004553)
Family alignment:	View or CHROMA formatHMM/Web logoCLUSTALW formatMSF formatFASTA formatPIR format

There are 16062 Glyco_32 domains in 15991 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Taxonomic distribution of proteins containing Glyco_32 domain.

  This tree includes only several representative species. The complete taxonomic breakdown of all proteins with Glyco_32 domain is also avaliable.

  Click on the protein counts, or double click on taxonomic names to display all proteins containing Glyco_32 domain in the selected taxonomic class.

• Metabolism (metabolic pathways involving proteins which contain this domain)

• Click the image to view the interactive version of the map in iPath

  % proteins involved KEGG pathway ID Description 51.37 map00500 Starch and sucrose metabolism 45.49 map00052 Galactose metabolism 2.35 map00530 Aminosugars metabolism 0.78 map00051 Fructose and mannose metabolism This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with Glyco_32 domain which could be assigned to a KEGG orthologous group, and not all proteins containing Glyco_32 domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

• Structure (3D structures containing this domain)

• 3D Structures of Glyco_32 domains in PDB

  PDB code	Main view	Title
  1st8		Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus
  1uyp		The three-dimensional structure of beta-fructosidase (invertase) from Thermotoga maritima
  1w2t		beta-fructosidase from Thermotoga maritima in complex with raffinose
  1y4w		Crystal structure of exo-inulinase from Aspergillus awamori in spacegroup P21
  1y9g		Crystal structure of exo-inulinase from Aspergillus awamori complexed with fructose
  1y9m		Crystal structure of exo-inulinase from Aspergillus awamori in spacegroup P212121
  2ac1		Crystal structure of a cell-wall invertase from Arabidopsis thaliana
  2add		Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus in complex with sucrose
  2ade		Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus in complex with fructose
  2aey		Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus in complex with 2,5 dideoxy-2,5-immino-D-mannitol
  2aez		Crystal structure of fructan 1-exohydrolase IIa (E201Q) from Cichorium intybus in complex with 1-kestose
  2oxb		Crystal structure of a cell-wall invertase (E203Q) from Arabidopsis thaliana in complex with sucrose
  2qqu		Crystal structure of a cell-wall invertase (D239A) from Arabidopsis thaliana in complex with sucrose
  2qqv		Crystal structure of a cell-wall invertase (E203A) from Arabidopsis thaliana in complex with sucrose
  2qqw		Crystal structure of a cell-wall invertase (D23A) from Arabidopsis thaliana in complex with sucrose
  2xqr		Crystal structure of plant cell wall invertase in complex with a specific protein inhibitor
  2yfr		Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533
  2yfs		Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533 in complex with sucrose
  2yft		Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533 in complex with 1-kestose
  3kf3		Structure of fructofuranosidase from Schwanniomyces occidentalis complexed with fructose
  3kf5		Structure of invertase from Schwanniomyces occidentalis
  3ldk		Crystal Structure of A. japonicus CB05
  3ldr		Crystal structure of fructosyltransferase (D191A) from A. japonicus in complex with 1-Kestose
  3lem		Crystal structure of fructosyltransferase (D191A) from A. japonicus in complex with Nystose
  3lf7		Crystal structure of fructosyltransferase (wild-type) from A. japonicus
  3lfi		Crystal structure of fructosyltransferase (wild-type) from A. japonicus in complex with glucose
  3lig		Crystal structure of fructosyltransferase (D191A) from A. japonicus
  3lih		Crystal structure of fructosyltransferase (D191A) from A. japonicus in complex with raffinose
  3pig		beta-fructofuranosidase from Bifidobacterium longum
  3pij		beta-fructofuranosidase from Bifidobacterium longum - complex with fructose
  3rwk		First crystal structure of an endo-inulinase, from Aspergillus ficuum: structural analysis and comparison with other GH32 enzymes.
  3sc7		First crystal structure of an endo-inulinase, from Aspergillus ficuum: structural analysis and comparison with other GH32 enzymes.
  3u14		Structure of D50A-fructofuranosidase from Schwanniomyces occidentalis complexed with inulin
  3u75		Structure of E230A-fructofuranosidase from Schwanniomyces occidentalis complexed with fructosylnystose
  3ugf		Crystal structure of a 6-SST/6-SFT from Pachysandra terminalis
  3ugg		Crystal structure of a 6-SST/6-SFT from Pachysandra terminalis in complex with 1-kestose
  3ugh		Crystal structure of a 6-SST/6-SFT from Pachysandra terminalis in complex with 6-kestose
  4eqv		Structure of Saccharomyces cerevisiae invertase
  4fff		Crystal Structure of Levan Fructotransferase from Arthrobacter ureafaciens
  4ffg		Crystal Structure of Levan Fructotransferase from Arthrobacter ureafaciens in complex with DFA-IV
  4ffh		Crystal Structure of Levan Fructotransferase D54N mutant from Arthrobacter ureafaciens in complex with sucrose
  4ffi		Crystal Structure of Levan Fructotransferase D54N mutant from Arthrobacter ureafaciens in complex with levanbiose
  5ann		5ANN
  5fix		5FIX
  5fk7		5FK7
  5fk8		5FK8
  5fkb		5FKB
  5fkc		5FKC
  5fmb		5FMB
  5fmc		5FMC
  5fmd		5FMD

• Links (links to other resources describing this domain)

• INTERPRO	IPR001362
  PFAM	Glyco_hydro_32

Send comments to Ivica Letunic