Schultz et al. (1998) Proc. Natl. Acad. Sci. USA 95, 5857-5864
Letunic et al. (2012) Nucleic Acids Res , doi:10.1093/nar/gkr931

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HTH_CRP helix_turn_helix, cAMP Regulatory protein

SMART accession number:	SM00419
Description:
Interpro abstract (IPR001808):	Numerous bacterial transcription regulatory proteins bind DNA via a helix-turn-helix (HTH) motif. These proteins are very diverse, but for convenience may be grouped into subfamilies on the basis of sequence similarity. This family groups together a range of proteins, including anr, crp, clp, cysR, fixK, flp, fnr, fnrN, hlyX and ntcA [(PUBMED:14638413), (PUBMED:10550204)]. Within this family, the HTH motif is situated towards the C terminus.
GO process:	regulation of transcription, DNA-dependent (GO:0006355)
GO component:	intracellular (GO:0005622)
GO function:	sequence-specific DNA binding transcription factor activity (GO:0003700)
Family alignment:	View or CHROMA formatCLUSTALW formatMSF formatFASTA formatPIR format

There are 2443 HTH_CRP domains in 2443 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Click on to expand nodes. To display all proteins with a HTH_CRP domain in a specific node, click on it.

  This tree shows only several representative species. The complete taxonomic breakdown of all proteins with HTH_CRP domain is also avaliable.

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• Literature (relevant references for this domain)

• Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.

  • McKay DB, Steitz TA
  • Structure of catabolite gene activator protein at 2.9 A resolution suggests binding to left-handed B-DNA.
  • Nature. 1981; 290: 744-9
  • Display abstract

  • The 2.9 A resolution crystal structure of Escherichia coli catabolite gene activator protein (CAP) complexed with cyclic AMP reveals two distinct structural domains separated by a cleft. The smaller carboxy-terminal domain is presumed to bind DNA while the amino-terminal domain is seen to bind cyclic AMP. Model building studies suggest that CAP binds to left-handed B-type DNA, contracting its major groove via two alpha-helices. It is possible that the CAP conversion of right- to left-handed DNA in a closed supercoil, is what activates transcription by RNA polymerase.

• Metabolism (metabolic pathways involving proteins which contain this domain)

• Click the image to view the interactive version of the map in iPath

  % proteins involved KEGG pathway ID Description 50.00 map00562 Inositol phosphate metabolism 50.00 map00632 Benzoate degradation via CoA ligation This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with HTH_CRP domain which could be assigned to a KEGG orthologous group, and not all proteins containing HTH_CRP domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

• Structure (3D structures containing this domain)

• 3D Structures of HTH_CRP domains in PDB

  PDB code	Main view	Title
  1cgp		Catabolite gene activator protein (cap)/dna complex + adenosine-3',5'-cyclic-monophosphate
  1ft9		Structure of the reduced (feii) co-sensing protein from r. rubrum
  1g6n		2.1 angstrom structure of cap-camp
  1hw5		The cap/crp variant t127l/s128a
  1i5z		Structure of crp-camp at 1.9 a
  1i6x		Structure of a star mutant crp-camp at 2.2 a
  1j59		Catabolite gene activator protein (cap)/dna complex + adenosine-3',5'-cyclic-monophosphate
  1lb2		Structure of the e. coli alpha c-terminal domain of rna polymerase in complex with cap and dna
  1o3q		Protein-dna recognition and dna deformation revealed in crystal structures of cap-dna complexes
  1o3r		Protein-dna recognition and dna deformation revealed in crystal structures of cap-dna complexes
  1o3s		Protein-dna recognition and dna deformation revealed in crystal structures of cap-dna complexes
  1o3t		Protein-dna recognition and dna deformation revealed in crystal structures of cap-dna complexes
  1o5l		Crystal structure of transcriptional regulator (tm1171) from thermotoga maritima at 2.30 a resolution
  1run		Catabolite gene activator protein (cap)/dna complex + adenosine-3',5'-cyclic-monophosphate
  1ruo		Catabolite gene activator protein (cap) mutant/dna complex + adenosine-3',5'-cyclic-monophosphate
  1zrc		Crystal structures of cap-dna with all base-pair substitutions at position 6, cap-icap38 dna
  1zrd		Crystal structures of cap-dna with all base-pair substitutions at position 6, cap-[6a;17t]icap38 dna
  1zre		Crystal structures of cap-dna with all base-pair substitutions at position 6, cap-[6g;17c]icap38 dna
  1zrf		Crystal structures of cap-dna with all base-pair substitutions at position 6, cap-[6c;17g]icap38 dna
  2cgp		Catabolite gene activator protein/dna complex, adenosine-3', 5'-cyclic-monophosphate
  2fmy		Co-dependent transcription factor cooa from carboxydothermus hydrogenoformans (imidazole-bound form)
  2gau		Crystal structure of transcriptional regulator, crp/fnr family from porphyromonas gingivalis (apc80792), structural genomics, mcsg
  2gzw		Crystal structure of the e.coli crp-camp complex
  2h6b		Crystal structure of oxidized cprk in complex with o- chlorophenolacetic acid
  2hkx		Structure of cooa mutant (n127l/s128l) from carboxydothermus hydrogenoformans
  2oz6		Crystal structure of virulence factor regulator from pseudomonas aeruginosa in complex with camp
  2wc2		Nmr structure of catabolite activator protein in the unliganded state
  2zcw		Crystal structure of ttha1359, a transcriptional regulator, crp/fnr family from thermus thermophilus hb8
  2zdb		Crystal structure of tthb099, a transcriptional regulator crp family from thermus thermophilus hb8
  3d0s		Camp receptor protein from m.tuberculosis, camp-free form
  3dkw		Crystal structure of dnr from pseudomonas aeruginosa.
  3dv8		Crystal structure of transcriptional regulator, crp/fnr family (rer070207001219) from eubacterium rectale at 2.55 a resolution
  3e5q		Unbound oxidised cprk
  3e5u		Ocpa complexed cprk (c200s)
  3e5x		Ocpa complexed cprk
  3e6b		Ocpa complexed cprk (c200s)
  3e6c		Cprk ocpa dna complex
  3e6d		Crystal structure of cprk c200s
  3e97		Crystal structure of transcriptional regulator of crp/fnr family (yp_604437.1) from deinococcus geothermalis dsm at 1.86 a resolution
  3fwe		Crystal structure of the apo d138l cap mutant
  3fx3		Structure of a putative camp-binding regulatory protein from silicibacter pomeroyi dss-3
  3h3z		Crystal structure of putative camp-binding regulatory protein (yp_165150.1) from silicibacter pomeroyi dss-3 at 2.35 a resolution
  3hif		The crystal structure of apo wild type cap at 3.6 a resolution.
  3i54		Crystal structure of mtbcrp in complex with camp
  3i59		Crystal structure of mtbcrp in complex with n6-camp
  3iyd		Three-dimensional em structure of an intact activator- dependent transcription initiation complex
  3kcc		Crystal structure of d138l mutant of catabolite gene activator protein

• Links (links to other resources describing this domain)

• PFAM	crp
  INTERPRO	IPR001808
  PROSITE	CNMP_BINDING_3

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