HTH_CRPhelix_turn_helix, cAMP Regulatory protein
|SMART accession number:||SM00419|
|Interpro abstract (IPR001808):||Numerous bacterial transcription regulatory proteins bind DNA via a helix-turn-helix (HTH) motif. These proteins are very diverse, but for convenience may be grouped into subfamilies on the basis of sequence similarity. This family groups together a range of proteins, including anr, crp, clp, cysR, fixK, flp, fnr, fnrN, hlyX and ntcA [(PUBMED:14638413), (PUBMED:10550204)]. Within this family, the HTH motif is situated towards the C terminus.|
|GO process:||regulation of transcription, DNA-templated (GO:0006355)|
|GO component:||intracellular (GO:0005622)|
|GO function:||sequence-specific DNA binding transcription factor activity (GO:0003700)|
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- Evolution (species in which this domain is found)
Taxonomic distribution of proteins containing HTH_CRP domain.
This tree includes only several representative species. The complete taxonomic breakdown of all proteins with HTH_CRP domain is also avaliable.
Click on the protein counts, or double click on taxonomic names to display all proteins containing HTH_CRP domain in the selected taxonomic class.
- Literature (relevant references for this domain)
Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.
- McKay DB, Steitz TA
- Structure of catabolite gene activator protein at 2.9 A resolution suggests binding to left-handed B-DNA.
- Nature. 1981; 290: 744-9
- Display abstract
The 2.9 A resolution crystal structure of Escherichia coli catabolite gene activator protein (CAP) complexed with cyclic AMP reveals two distinct structural domains separated by a cleft. The smaller carboxy-terminal domain is presumed to bind DNA while the amino-terminal domain is seen to bind cyclic AMP. Model building studies suggest that CAP binds to left-handed B-type DNA, contracting its major groove via two alpha-helices. It is possible that the CAP conversion of right- to left-handed DNA in a closed supercoil, is what activates transcription by RNA polymerase.
- Metabolism (metabolic pathways involving proteins which contain this domain)
Click the image to view the interactive version of the map in iPath
% proteins involved KEGG pathway ID Description 50.00 map00562 Inositol phosphate metabolism 50.00 map00632 Benzoate degradation via CoA ligation
This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with HTH_CRP domain which could be assigned to a KEGG orthologous group, and not all proteins containing HTH_CRP domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.
- Structure (3D structures containing this domain)
3D Structures of HTH_CRP domains in PDB
PDB code Main view Title 1cgp CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE 1ft9 STRUCTURE OF THE REDUCED (FEII) CO-SENSING PROTEIN FROM R. RUBRUM 1g6n 2.1 ANGSTROM STRUCTURE OF CAP-CAMP 1hw5 THE CAP/CRP VARIANT T127L/S128A 1i5z STRUCTURE OF CRP-CAMP AT 1.9 A 1i6x STRUCTURE OF A STAR MUTANT CRP-CAMP AT 2.2 A 1j59 CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE 1lb2 Structure of the E. coli alpha C-terminal domain of RNA polymerase in complex with CAP and DNA 1o3q PROTEIN-DNA RECOGNITION AND DNA DEFORMATION REVEALED IN CRYSTAL STRUCTURES OF CAP-DNA COMPLEXES 1o3r PROTEIN-DNA RECOGNITION AND DNA DEFORMATION REVEALED IN CRYSTAL STRUCTURES OF CAP-DNA COMPLEXES 1o3s PROTEIN-DNA RECOGNITION AND DNA DEFORMATION REVEALED IN CRYSTAL STRUCTURES OF CAP-DNA COMPLEXES 1o3t PROTEIN-DNA RECOGNITION AND DNA DEFORMATION REVEALED IN CRYSTAL STRUCTURES OF CAP-DNA COMPLEXES 1o5l Crystal structure of Transcriptional regulator (TM1171) from Thermotoga maritima at 2.30 A resolution 1run CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE 1ruo CATABOLITE GENE ACTIVATOR PROTEIN (CAP) MUTANT/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE 1zrc 4 Crystal structures of CAP-DNA with all base-pair substitutions at position 6, CAP-ICAP38 DNA 1zrd 4 crystal structures of CAP-DNA with all base-pair substitutions at position 6, CAP-[6A;17T]ICAP38 DNA 1zre 4 crystal structures of CAP-DNA with all base-pair substitutions at position 6, CAP-[6G;17C]ICAP38 DNA 1zrf 4 crystal structures of CAP-DNA with all base-pair substitutions at position 6, CAP-[6C;17G]ICAP38 DNA 2cgp CATABOLITE GENE ACTIVATOR PROTEIN/DNA COMPLEX, ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE 2fmy CO-dependent transcription factor CooA from Carboxydothermus hydrogenoformans (Imidazole-bound form) 2gzw Crystal structure of the E.coli CRP-cAMP complex 2hkx Structure of CooA mutant (N127L/S128L) from Carboxydothermus hydrogenoformans 2oz6 Crystal Structure of Virulence Factor Regulator from Pseudomonas aeruginosa in complex with cAMP 2wc2 Nmr structure of catabolite activator protein in the unliganded state 2xgx Crystal structure of transcription factor NtcA from Synechococcus elongatus (mercury derivative) 2xhk Crystal structure of transcription factor NtcA from Synechococcus elongatus bound to 2-oxoglutarate 2xko Crystal structure of the complex of NtcA with its transcriptional co- activator PipX 2xkp NtcA from Synechococcus elongatus: active and inactive 2zcw Crystal Structure of TTHA1359, a Transcriptional Regulator, CRP/FNR family from Thermus thermophilus HB8 3b02 Crystal structure of TTHB099, a transcriptional regulator CRP family from Thermus thermophilus HB8 3d0s cAMP receptor protein from m.tuberculosis, cAMP-free form 3dkw Crystal Structure of DNR from Pseudomonas aeruginosa. 3dv8 Crystal structure of a putative transcriptional regulator of the crp/fnr family (eubrec_1222) from eubacterium rectale atcc 33656 at 2.55 A resolution 3e97 Crystal structure of transcriptional regulator of Crp/Fnr family (YP_604437.1) from DEINOCOCCUS GEOTHERMALIS DSM 11300 at 1.86 A resolution 3fwe Crystal Structure of the Apo D138L CAP mutant 3h3u Crystal structure of CRP (cAMP receptor Protein) from Mycobacterium tuberculosis 3hif The crystal structure of apo wild type CAP at 3.6 A resolution. 3i54 Crystal structure of MtbCRP in complex with cAMP 3i59 Crystal structure of MtbCRP in complex with N6-cAMP 3iwz The c-di-GMP Responsive Global Regulator CLP Links Cell-Cell Signaling to Virulence Gene Expression in Xanthomonas campestris 3iyd Three-dimensional EM structure of an intact activator-dependent transcription initiation complex 3kcc Crystal structure of D138L mutant of Catabolite Gene Activator Protein 3la2 Crystal structure of NtcA in complex with 2-oxoglutarate 3la3 Crystal structure of NtcA in complex with 2,2-difluoropentanedioic acid 3la7 Crystal structure of NtcA in apo-form 3mzh Crystal structure of cAMP receptor protein from mycobacterium tuberculosis in complex with cAMP and its DNA binding element 3n4m E. coli RNA polymerase alpha subunit C-terminal domain in complex with CAP and DNA 3qop Domain-domain flexibility leads to allostery within the camp receptor protein (CRP) 3r6s Crystal structure of GlxR transcription factor from Corynebacterium glutamicum with cAMP 3rdi Domain-domain flexibility leads to allostery within the camp receptor protein (CRP) 3rou Domain-domain flexibility leads to allostery within the camp receptor protein (CRP) 3rpq Domain-domain flexibility leads to allostery within the camp receptor protein (CRP) 3ryp Domain-domain flexibility leads to allostery within the camp receptor protein (CRP) 3ryr Domain-domain flexibility leads to allostery within the camp receptor protein (CRP) 4a2u CRP(CAP) from Myco. Tuberculosis, with cAMP 4bh9 A structural model of CAP mutant (T127L and S128I) in the apo state 4bhp A structural model of CAP mutant (T127L and S128I) in cGMP-bound state 4ev0 Crystal Structure of Thermus thermophilus Catabolite Activator Protein 4ft8 E. coli Catabolite Activator Protein with Cobalt and Sulfate Ligands 4hzf structure of the wild type Catabolite gene Activator Protein 4i01 Structure of the mutant Catabolite gen activator protein V140L 4i02 structure of the mutant Catabolite gene activator protein V140A 4i09 structure of the mutant Catabolite gene activator protein V132L 4i0a structure of the mutant Catabolite gene activator protein V132A 4i0b structure of the mutant Catabolite gene activator protein H160L 4i2o The Structure of FixK2 from Bradyrhizobium japonicum
- Links (links to other resources describing this domain)
PFAM crp INTERPRO IPR001808 PROSITE CNMP_BINDING_3