KHK homology RNA-binding domain |
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| SMART accession number: | SM00322 |
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| Description: | |
| Interpro abstract (IPR004087): | The K homology (KH) domain was first identified in the human heterogeneous nuclear ribonucleoprotein (hnRNP) K. An evolutionarily conserved sequence of around 70 amino acids, the KH domain is present in a wide variety of nucleic acid-binding proteins. The KH domain binds RNA, and can function in RNA recognition [(PUBMED:17437720)]. It is found in multiple copies in several proteins, where they can function cooperatively or independently. For example, in the AU-rich element RNA-binding protein KSRP, which has 4 KH domains, KH domains 3 and 4 behave as independent binding modules to interact with different regions of the AU-rich RNA targets [(PUBMED:17437720)]. The solution structure of the first KH domain of FMR1 [(PUBMED:9302998)] and of the C-terminal KH domain of hnRNP K [(PUBMED:10369774)] determined by nuclear magnetic resonance (NMR) revealed a beta-alpha-alpha-beta-beta-alpha structure. Proteins containing KH domains include:
According to structural analyses [(PUBMED:9302998), (PUBMED:10369774), (PUBMED:11160884)], the KH domain can be separated in two groups - type 1 and type 2. |
| GO function: | RNA binding (GO:0003723) |
| Family alignment: |
There are 16184 KH domains in 10163 proteins in SMART's nrdb database.
Click on the following links for more information.
- Evolution (species in which this domain is found)
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Go to specific node: Anopheles gambiae, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Homo sapiens, Mus musculus, Rattus norvegicus, Saccharomyces cerevisiae, Takifugu rubripes - Cellular role (predicted cellular role)
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Binding / catalysis: RNA-binding
- Literature (relevant references for this domain)
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Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.
- Lewis HA et al.
- Sequence-specific RNA binding by a Nova KH domain: implications for paraneoplastic disease and the fragile X syndrome.
- Cell. 2000; 100: 323-32
- Display abstract
The structure of a Nova protein K homology (KH) domain recognizing single-stranded RNA has been determined at 2.4 A resolution. Mammalian Nova antigens (1 and 2) constitute an important family of regulators of RNA metabolism in neurons, first identified using sera from cancer patients with the autoimmune disorder paraneoplastic opsoclonus-myoclonus ataxia (POMA). The structure of the third KH domain (KH3) of Nova-2 bound to a stem loop RNA resembles a molecular vise, with 5'-Ura-Cyt-Ade-Cyt-3' pinioned between an invariant Gly-X-X-Gly motif and the variable loop. Tetranucleotide recognition is supported by an aliphatic alpha helix/beta sheet RNA-binding platform, which mimics 5'-Ura-Gua-3' by making Watson-Crick-like hydrogen bonds with 5'-Cyt-Ade-3'. Sequence conservation suggests that fragile X mental retardation results from perturbation of RNA binding by the FMR1 protein.
- Adinolfi S, Bagni C, Musco G, Gibson T, Mazzarella L, Pastore A
- Dissecting FMR1, the protein responsible for fragile X syndrome, in its structural and functional domains.
- RNA. 1999; 5: 1248-58
- Display abstract
FMR1 is an RNA-binding protein that is either absent or mutated in patients affected by the fragile X syndrome, the most common inherited cause of mental retardation in humans. Sequence analysis of the FMR1 protein has suggested that RNA binding is related to the presence of two K-homologous (KH) modules and an RGG box. However, no attempt has been so far made to map the RNA-binding sites along the protein sequence and to identify possible differential RNA-sequence specificity. In the present article, we describe work done to dissect FMR1 into regions with structurally and functionally distinct properties. A semirational approach was followed to identify four regions: an N-terminal stretch of 200 amino acids, the two KH regions, and a C-terminal stretch. Each region was produced as a recombinant protein, purified, and probed for its state of folding by spectroscopical techniques. Circular dichroism and NMR spectra of the N-terminus show formation of secondary structure with a strong tendency to aggregate. Of the two homologous KH motifs, only the first one is folded whereas the second remains unfolded even when it is extended both N- and C-terminally. The C-terminus is, as expected from its amino acid composition, nonglobular. Binding assays were then performed using the 4-nt homopolymers. Our results show that only the first KH domain but not the second binds to RNA, and provide the first direct evidence for RNA binding of both the N-terminal and the C-terminal regions. RNA binding for the N-terminus could not be predicted from sequence analysis because no known RNA-binding motif is identifiable in this region. Different sequence specificity was observed for the fragments: both the N-terminus of the protein and KH1 bind preferentially to poly-(rG). The C-terminal region, which contains the RGG box, is nonspecific, as it recognizes the bases with comparable affinity. We therefore conclude that FMR1 is a protein with multiple sites of interaction with RNA: sequence specificity is most likely achieved by the whole block that comprises the first approximately 400 residues, whereas the C-terminus provides a nonspecific binding surface.
- Ostareck-Lederer A, Ostareck DH, Hentze MW
- Cytoplasmic regulatory functions of the KH-domain proteins hnRNPs K and E1/E2.
- Trends Biochem Sci. 1998; 23: 409-11
- Musco G et al.
- The solution structure of the first KH domain of FMR1, the protein responsible for the fragile X syndrome.
- Nat Struct Biol. 1997; 4: 712-6
- Musco G et al.
- Three-dimensional structure and stability of the KH domain: molecular insights into the fragile X syndrome.
- Cell. 1996; 85: 237-45
- Display abstract
The KH module is a sequence motif found in a number of proteins that are known to be in close association with RNA. Experimental evidence suggests a direct involvement of KH in RNA binding. The human FMR1 protein, which has two KH domains, is associated with fragile X syndrome, the most common inherited cause of mental retardation. Here we present the three-dimensional solution structure of the KH module. The domain consists of a stable beta alpha alpha beta beta alpha fold. On the basis of our results, we suggest a potential surface for RNA binding centered on the loop between the first two helices. Substitution of a well-conserved hydrophobic residue located on the second helix destroys the KH fold; a mutation of this position in FMR1 leads to an aggravated fragile X phenotype.
- Siomi H, Choi M, Siomi MC, Nussbaum RL, Dreyfuss G
- Essential role for KH domains in RNA binding: impaired RNA binding by a mutation in the KH domain of FMR1 that causes fragile X syndrome.
- Cell. 1994; 77: 33-9
- Display abstract
The KH domain is an evolutionarily conserved sequence motif present in many RNA-binding proteins, including the pre-mRNA-binding (hnRNP) K protein and the fragile X mental retardation gene product (FMR1). We assessed the role of KH domains in RNA binding by mutagenesis of KH domains in hnRNP K and FMR1. Conserved residues of all three hnRNP K KH domains are required for its wild-type RNA binding. Interestingly, while fragile X syndrome is usually caused by lack of FMR1 expression, a previously reported mutation in a highly conserved residue of one of its two KH domains (Ile-304-->Asn) also results in mental retardation. We found that the binding of this mutant protein to RNA is severely impaired. These results demonstrate an essential role for KH domains in RNA binding. Furthermore, they strengthen the connection between fragile X syndrome and loss of the RNA binding activity of FMR1.
- Disease (disease genes where sequence variants are found in this domain)
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SwissProt sequences and OMIM curated human diseases associated with missense mutations within the KH domain.
Protein Disease Fragile X mental retardation 1 protein (Q06787) (SMART) OMIM:309550: Fragile X syndrome - Metabolism (metabolic pathways involving proteins which contain this domain)
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Click the image to view the interactive version of the map in iPath% proteins involved KEGG pathway ID Description 34.62 
map00240Pyrimidine metabolism 34.62 map00230Purine metabolism 29.68 map03010 Ribosome 1.00 map02010 ABC transporters - General 0.08 map00190Oxidative phosphorylation This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with KH domain which could be assigned to a KEGG orthologous group, and not all proteins containing KH domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.
- Structure (3D structures containing this domain)
3D Structures of KH domains in PDB
PDB code Main view Title 1dt4 
Crystal structure of nova-1 kh3 k-homology rna-binding domain 1dtj 
Crystal structure of nova-2 kh3 k-homology rna-binding domain 1e3h 
Semet derivative of streptomyces antibioticus pnpase/gpsi enzyme 1e3p 
Tungstate derivative of streptomyces antibioticus pnpase/ gpsi enzyme 1ec6 
Crystal structure of nova-2 kh3 k-homology rna-binding domain bound to 20-mer rna hairpin 1fjg 
Structure of the thermus thermophilus 30s ribosomal subunit in complex with the antibiotics streptomycin, spectinomycin, and paromomycin 1gix 
Crystal structure of the ribosome at 5.5 a resolution. this file, 1gix, contains the 30s ribosome subunit, three trna, and mrna molecules. 50s ribosome subunit is in the file 1giy 1hh2 
Crystal structure of nusa from thermotoga maritima 1hnw 
Structure of the thermus thermophilus 30s ribosomal subunit in complex with tetracycline 1hnx 
Structure of the thermus thermophilus 30s ribosomal subunit in complex with pactamycin 1hnz 
Structure of the thermus thermophilus 30s ribosomal subunit in complex with hygromycin b 1hr0 
Crystal structure of initiation factor if1 bound to the 30s ribosomal subunit 1i94 
Crystal structures of the small ribosomal subunit with tetracycline, edeine and if3 1i95 
Crystal structure of the 30s ribosomal subunit from thermus thermophilus in complex with edeine 1i96 
Crystal structure of the 30s ribosomal subunit from thermus thermophilus in complex with the translation initiation factor if3 (c-terminal domain) 1i97 
Crystal structure of the 30s ribosomal subunit from thermus thermophilus in complex with tetracycline 1ibk 
Structure of the thermus thermophilus 30s ribosomal subunit in complex with the antibiotic paromomycin 1ibl 
Structure of the thermus thermophilus 30s ribosomal subunit in complex with a messenger rna fragment and cognate transfer rna anticodon stem-loop bound at the a site and with the antibiotic paromomycin 1ibm 
Structure of the thermus thermophilus 30s ribosomal subunit in complex with a messenger rna fragment and cognate transfer rna anticodon stem-loop bound at the a site 1j4w 
Complex of the kh3 and kh4 domains of fbp with a single_stranded 29mer dna oligonucleotide from the fuse element of the c-myc oncogene 1j5e 
Structure of the thermus thermophilus 30s ribosomal subunit 1j5k 
Complex of the kh3 domain of hnrnp k with a single_stranded 10mer dna oligonucleotide 1jgo 
The path of messenger rna through the ribosome. this file, 1jgo, contains the 30s ribosome subunit, three trna, and mrna molecules. 50s ribosome subunit is in the file 1giy 1jgp 
The path of messenger rna through the ribosome. this file, 1jgp, contains the 30s ribosome subunit, three trna, and mrna molecules. 50s ribosome subunit is in the file 1giy 1jgq 
The path of messenger rna through the ribosome. this file, 1jgq, contains the 30s ribosome subunit, three trna, and mrna molecules. 50s ribosome subunit is in the file 1giy 1k0r 
Crystal structure of mycobacterium tuberculosis nusa 1k1g 
Structural basis for recognition of the intron branch site rna by splicing factor 1 1khm 
C-terminal kh domain of hnrnp k (kh3) 1l2f 
Crystal structure of nusa from thermotoga maritima: a structure-based role of the n-terminal domain 1ml5 
Structure of the e. coli ribosomal termination complex with release factor 2 1n32 
Structure of the thermus thermophilus 30s ribosomal subunit bound to codon and near-cognate transfer rna anticodon stem-loop mismatched at the first codon position at the a site with paromomycin 1n33 
Structure of the thermus thermophilus 30s ribosomal subunit bound to codon and near-cognate transfer rna anticodon stem-loop mismatched at the second codon position at the a site with paromomycin 1n34 
Structure of the thermus thermophilus 30s ribosomal subunit in the presence of codon and crystallographically disordered near-cognate transfer rna anticodon stem-loop mismatched at the first codon position 1n36 
Structure of the thermus thermophilus 30s ribosomal subunit in the presence of crystallographically disordered codon and near-cognate transfer rna anticodon stem-loop mismatched at the second codon position 1p6g 
Real space refined coordinates of the 30s subunit fitted into the low resolution cryo-em map of the ef-g.gtp state of e. coli 70s ribosome 1p87 
Real space refined coordinates of the 30s subunit fitted into the low resolution cryo-em map of the initiation-like state of e. coli 70s ribosome 1pns 
Crystal structure of a streptomycin dependent ribosome from e. coli, 30s subunit of 70s ribosome. this file, 1pns, contains the 30s subunit, two trnas, and one mrna molecule. the 50s ribosomal subunit is in file 1pnu. 1pnx 
Crystal structure of the wild type ribosome from e. coli, 30s subunit of 70s ribosome. this file, 1pnx, contains only molecules of the 30s ribosomal subunit. the 50s subunit is in the pdb file 1pny. 1s1h 
Structure of the ribosomal 80s-eef2-sordarin complex from yeast obtained by docking atomic models for rna and protein components into a 11.7 a cryo-em map. this file, 1s1h, contains 40s subunit. the 60s ribosomal subunit is in file 1s1i. 1tua 
1.5 a crystal structure of a protein of unknown function ape0754 from aeropyrum pernix 1vig 
Nmr study of vigilin, repeat 6, 40 structures 1vih 
Nmr study of vigilin, repeat 6, minimized average structure 1voq 
Crystal structure of five 70s ribosomes from escherichia coli in complex with protein y. this file contains the 30s subunit of one 70s ribosome. the entire crystal structure contains five 70s ribosomes and is described in remark 400. 1vos 
Crystal structure of five 70s ribosomes from escherichia coli in complex with protein y. this file contains the 30s subunit of one 70s ribosome. the entire crystal structure contains five 70s ribosomes and is described in remark 400. 1vov 
Crystal structure of five 70s ribosomes from escherichia coli in complex with protein y. this file contains the 30s subunit of one 70s ribosome. the entire crystal structure contains five 70s ribosomes and is described in remark 400. 1vox 
Crystal structure of five 70s ribosomes from escherichia coli in complex with protein y. this file contains the 30s subunit of one 70s ribosome. the entire crystal structure contains five 70s ribosomes and is described in remark 400. 1voz 
Crystal structure of five 70s ribosomes from escherichia coli in complex with protein y. this file contains the 30s subunit of one 70s ribosome. the entire crystal structure contains five 70s ribosomes and is described in remark 400. 1vs5 
Crystal structure of the bacterial ribosome from escherichia coli in complex with the antibiotic kasugamyin at 3.5a resolution. this file contains the 30s subunit of one 70s ribosome. the entire crystal structure contains two 70s ribosomes and is described in remark 400. 1vs7 
Crystal structure of the bacterial ribosome from escherichia coli in complex with the antibiotic kasugamyin at 3.5a resolution. this file contains the 30s subunit of one 70s ribosome. the entire crystal structure contains two 70s ribosomes and is described in remark 400. 1we8 
Solution structure of kh domain in protein bab28342 1wvn 
Crsytal structure of domain 3 of human alpha polyc binding protein 1x4m 
Solution structure of kh domain in far upstream element binding protein 1 1x4n 
Solution structure of kh domain in fuse binding protein 1 1xmo 
Crystal structure of mnm5u34t6a37-trnalysuuu complexed with aag-mrna in the decoding center 1xmq 
Crystal structure of t6a37-asllysuuu aaa-mrna bound to the decoding center 1xnq 
Structure of an inosine-adenine wobble base pair complex in the context of the decoding center 1xnr 
Crystal structure of an inosine-cytosine wobble base pair in the context of the decoding center 1yl4 
Crystal structure of 70s ribosome with thrs operator and trnas. 30s subunit. the coordinates for the 50s subunit are in the pdb entry 1yl3 1ztg 
Human alpha polyc binding protein kh1 1zzi 
Crystal structure analysis of the third kh domain of hnrnp k in complex with ssdna 1zzj 
Structure of the third kh domain of hnrnp k in complex with 15-mer ssdna 1zzk 
Crystal structure of the third kh domain of hnrnp k at 0.95a resolution 2ann 
Crystal structure (i) of nova-1 kh1/kh2 domain tandem with nt rna hairpin 2anr 
Crystal structure (ii) of nova-1 kh1/kh2 domain tandem with 25nt rna hairpin 2asb 
Structure of a mycobacterium tuberculosis nusa-rna complex 2atw 
Structure of a mycobacterium tuberculosis nusa-rna complex 2avy 
Crystal structure of the bacterial ribosome from escherichia coli at 3.5 a resolution. this file contains the 30s subunit of one 70s ribosome. the entire crystal structure contains two 70s ribosomes and is described in remark 400. 2aw7 
Crystal structure of the bacterial ribosome from escherichia coli at 3.5 a resolution. this file contains the 30s subunit of the second 70s ribosome. the entire crystal structure contains two 70s ribosomes and is described in remark 400. 2axy 
Crystal structure of kh1 domain of human poly(c)-binding protein-2 with c-rich strand of human telomeric dna 2b64 
30s ribosomal subunit, trnas, mrna and release factor rf1 from a crystal structure of the whole ribosomal complex. this file contains the 30s subunit, trnas, mrna and release factor rf1 from a crystal structure of the whole ribosomal complex". the entire crystal structure contains one 70s ribosome, trnas, mrna and release factor rf1 and is described in remark 400. 2b9m 
30s ribosomal subunit, trnas, mrna and release factor rf2 from a crystal structure of the whole ribosomal complex. this file contains the 30s ribosomal subunit, trnas, mrna and release factor rf2 from a crystal structure of the whole ribosomal complex". the entire crystal structure contains one 70s ribosome, trnas, mrna and release factor rf2 and is described in remark 400. 2b9o 
30s ribosomal subunit, trnas and mrna from a crystal structure of the whole ribosomal complex with a stop codon in the a-site. this file contains the 30s subunit, trnas and mrna from a crystal structure of the whole ribosomal complex with a stop codon in the a-site and is described in remark 400. 2ba0 
Archaeal exosome core 2bl5 
Solution structure of the kh-qua2 region of the xenopus star-gsg quaking protein. 2cpq 
Solution structure of the n-terminal kh domain of human fxr1 2cte 
Solution structure of the 1st kh type i domain from human vigilin 2ctf 
Solution structure of the 4th kh type i domain from human vigilin 2ctj 
Solution structure of the 8th kh type i domain from human vigilin 2ctk 
Solution structure of the 12th kh type i domain from human vigilin 2ctl 
Solution structure of the 13th kh type i domain from human vigilin 2ctm 
Solution structure of the 14th kh type i domain from human vigilin 2cxc 
Crystal structure of archaeal transcription termination factor nusa 2cy1 
Crystal structure of ape1850 2dgr 
Solution structure of the second kh domain in ring finger and kh domain containing protein 1 2e3u 
Crystal structure analysis of dim2p from pyrococcus horikoshii ot3 2e5l 
A snapshot of the 30s ribosomal subunit capturing mrna via the shine- dalgarno interaction 2f4v 
30s ribosome + designer antibiotic 2fmr 
Kh1 from the fragile x protein fmr1, nmr, 18 structures 2gy9 
Structure of the 30s subunit of a pre-translocational e. coli ribosome obtained by fitting atomic models for rna and protein components into cryo-em map emd-1056 2gyb 
Structure of the 30s subunit of a secm-stalled e. coli ribosome complex obtained by fitting atomic models for rna and protein components into cryo-em map emd-1143 2hgi 
Crystal structure of the 70s thermus thermophilus ribosome showing how the 16s 3'-end mimicks mrna e and p codons. this entry 2hgi contains 30s ribosomal subunit. the 50s ribosomal subunit can be found in pdb entry 2hgj. 2hgp 
Crystal structure of the 70s thermus thermophilus ribosome with translocated and rotated shine-dalgarno duplex. this entry 2hgp contains 30s ribosomal subunit. the 50s ribosomal subunit can be found in pdb entry 2hgq. 2hgr 
70s t.th. ribosome functional complex with mrna and e- and p-site trnas at 4.5a. this entry 2hgr contains 30s ribosomal subunit. the 50s ribosomal subunit can be found in pdb entry 2hgu. 2hh2 
Solution structure of the fourth kh domain of ksrp 2hh3 
Solution structure of the third kh domain of ksrp 2hhh 
Crystal structure of kasugamycin bound to the 30s ribosomal subunit 2i2p 
Crystal structure of ribosome with messenger rna and the anticodon stem-loop of p-site trna. this file contains the 30s subunit of one 70s ribosome. the entire crystal structure contains two 70s ribosomes and is described in remark 400. 2i2u 
Crystal structure of ribosome with messenger rna and the anticodon stem-loop of p-site trna. this file contains the 30s subunit of one 70s ribosome. the entire crystal structure contains two 70s ribosomes and is described in remark 400. 2j00 
Structure of the thermus thermophilus 70s ribosome complexed with mrna, trna and paromomycin (part 1 of 4). this file contains the 30s subunit, mrna, a-, p- and e-site trnas and paromomycin for molecule i. 2j02 
Structure of the thermus thermophilus 70s ribosome complexed with mrna, trna and paromomycin (part 3 of 4) this file contains the 30s subunit, mrna, a-, p- and e-site trnas and paromomycin for molecule ii. 2je6 
Structure of a 9-subunit archaeal exosome 2jea 
Structure of a 9-subunit archaeal exosome bound to rna 2jeb 
Structure of a 9-subunit archaeal exosome bound to mn ions 2jvz 
Solution nmr structure of the second and third kh domains of ksrp 2jzx 
Pcbp2 kh1-kh2 domains 2opu 
Solution nmr structure of the first domain of ksrp 2opv 
Solution nmr structure of the second domain of ksrp 2ow8 
Crystal structure of a 70s ribosome-trna complex reveals functional interactions and rearrangements. this file, 2ow8, contains the 30s ribosome subunit, two trna, and mrna molecules. 50s ribosome subunit is in the file 1vsa. 2p2r 
Crystal structure of the third kh domain of human poly(c)- binding protein-2 in complex with c-rich strand of human telomeric dna 2pqu 
Crystal structure of kh1 domain of human pcbp2 complexed to single-stranded 12-mer telomeric dna 2py9 
Protein-rna interaction involving kh1 domain from human poly(c)-binding protein-2 2qal 
Crystal structure of the bacterial ribosome from escherichia coli in complex with neomycin. this file contains the 30s subunit of the first 70s ribosome, with neomycin bound. the entire crystal structure contains two 70s ribosomes and is described in remark 400. 2qan 
Crystal structure of the bacterial ribosome from escherichia coli in complex with neomycin. this file contains the 30s subunit of the second 70s ribosome, with neomycin bound. the entire crystal structure contains two 70s ribosomes and is described in remark 400. 2qb9 
Crystal structure of the bacterial ribosome from escherichia coli in complex with gentamicin. this file contains the 30s subunit of the first 70s ribosome, with gentamicin bound. the entire crystal structure contains two 70s ribosomes and is described in remark 400. 2qbb 
Crystal structure of the bacterial ribosome from escherichia coli in complex with gentamicin. this file contains the 30s subunit of the second 70s ribosome, with gentamicin bound. the entire crystal structure contains two 70s ribosomes and is described in remark 400. 2qbd 
Crystal structure of the bacterial ribosome from escherichia coli in complex with ribosome recycling factor (rrf). this file contains the 30s subunit of the first 70s ribosome. the entire crystal structure contains two 70s ribosomes and is described in remark 400. 2qbf 
Crystal structure of the bacterial ribosome from escherichia coli in complex with ribosome recycling factor (rrf). this file contains the 30s subunit of the second 70s ribosome. the entire crystal structure contains two 70s ribosomes and is described in remark 400. 2qbh 
Crystal structure of the bacterial ribosome from escherichia coli in complex with gentamicin and ribosome recycling factor (rrf). this file contains the 30s subunit of the first 70s ribosome, with gentamicin bound. the entire crystal structure contains two 70s ribosomes and is described in remark 400. 2qbj 
Crystal structure of the bacterial ribosome from escherichia coli in complex with gentamicin and ribosome recycling factor (rrf). this file contains the 30s subunit of the second 70s ribosome, with gentamicin bound. the entire crystal structure contains two 70s ribosomes and is described in remark 400. 2qnd 
Crystal structure of the kh1-kh2 domains from human fragile x mental retardation protein 2qnh 
Interactions and dynamics of the shine-dalgarno helix in the 70s ribosome. this file, 2qnh, contains the 30s ribosome subunit, two trna, and mrna molecules. 50s ribosome subunit is in the file 1vsp. 2qou 
Crystal structure of the bacterial ribosome from escherichia coli in complex with spectinomycin. this file contains the 30s subunit of the first 70s ribosome, with spectinomycin bound. the entire crystal structure contains two 70s ribosomes. 2qow 
Crystal structure of the bacterial ribosome from escherichia coli in complex with spectinomycin. this file contains the 30s subunit of the second 70s ribosome, with spectinomycin bound. the entire crystal structure contains two 70s ribosomes. 2qoy 
Crystal structure of the bacterial ribosome from escherichia coli in complex with spectinomycin and neomycin. this file contains the 30s subunit of the first 70s ribosome, with spectinomycin and neomycin bound. the entire crystal structure contains two 70s ribosomes. 2qp0 
Crystal structure of the bacterial ribosome from escherichia coli in complex with spectinomycin and neomycin. this file contains the 30s subunit of the second 70s ribosome, with spectinomycin and neomycin bound. the entire crystal structure contains two 70s ribosomes. 2uu9 
Structure of the thermus thermophilus 30s ribosomal subunit complexed with a valine-asl with cmo5u in position 34 bound to an mrna with a gug-codon in the a-site and paromomycin. 2uua 
Structure of the thermus thermophilus 30s ribosomal subunit complexed with a valine-asl with cmo5u in position 34 bound to an mrna with a guc-codon in the a-site and paromomycin. 2uub 
Structure of the thermus thermophilus 30s ribosomal subunit complexed with a valine-asl with cmo5u in position 34 bound to an mrna with a guu-codon in the a-site and paromomycin. 2uuc 
Structure of the thermus thermophilus 30s ribosomal subunit complexed with a valine-asl with cmo5u in position 34 bound to an mrna with a gua-codon in the a-site and paromomycin. 2uxb 
Crystal structure of an extended trna anticodon stem loop in complex with its cognate mrna gggu in the context of the thermus thermophilus 30s subunit. 2uxc 
Crystal structure of an extended trna anticodon stem loop in complex with its cognate mrna ucgu in the context of the thermus thermophilus 30s subunit. 2uxd 
Crystal structure of an extended trna anticodon stem loop in complex with its cognate mrna cggg in the context of the thermus thermophilus 30s subunit. 2v46 
Structure of the ribosome recycling factor bound to the thermus thermophilus 70s ribosome with mrna, asl-phe and trna-fmet (part 1 of 4). this file contains the 30s subunit, mrna, p-site asl, e-site trna and rrf for molecule 1. 2v48 
Structure of the ribosome recycling factor bound to the thermus thermophilus 70s ribosome with mrna, asl-phe and trna-fmet (part 3 of 4). this file contains the 30s subunit, mrna, p-site asl, e-site trna and rrf for molecule 2. 2vho 
Structure of pdf binding helix in complex with the ribosome (part 3 of 4) 2vhp 
Structure of pdf binding helix in complex with the ribosome (part 4 of 4) 2vqe 
Modified uridines with c5-methylene substituents at the first position of the trna anticodon stabilize u-g wobble pairing during decoding 2vqf 
Modified uridines with c5-methylene substituents at the first position of the trna anticodon stabilize u-g wobble pairing during decoding 2wdg 
Structure of the thermus thermophilus 70s ribosome in complex with mrna, paromomycin, acylated a-site trna, deacylated p-site trna, and e-site trna. this file contains the 30s subunit a-,p-, and e-site trnas and paromomycin for molecule i. 2wdh 
Structure of the thermus thermophilus 70s ribosome in complex with mrna, paromomycin, acylated a-site trna, deacylated p-site trna, and e-site trna. this file contains the 30s subunit a-,p-, and e-site trnas and paromomycin for molecule ii. 2wdk 
Structure of the thermus thermophilus 70s ribosome in complex with mrna, paromomycin, acylated a- and p-site trnas, and e-site trna. this file contains the 30s subunit a-,p-, and e-site trnas and paromomycin for molecule i. 2wdm 
Structure of the thermus thermophilus 70s ribosome in complex with mrna, paromomycin, acylated a- and p-site trnas, and e-site trna. this file contains the 30s subunit a-,p-, and e-site trnas and paromomycin for molecule ii. 2wh1 
Insights into translational termination from the structure of rf2 bound to the ribosome 2wh3 
Insights into translational termination from the structure of rf2 bound to the ribosome). this file contains the 30s subunit. 2wri 
The structure of the ribosome with elongation factor g trapped in the post-translocational state (part 1 of 4). 2wrk 
The structure of the ribosome with elongation factor g trapped in the post-translocational state (part 3 of 4). 2wrn 
The crystal structure of the 70s ribosome bound to ef-tu and trna (part 1 of 4). 2wrq 
The crystal structure of the 70s ribosome bound to ef-tu and trna (part 3 of 4). 2z0s 
Crystal structure of putative exosome complex rna-binding protein 2z4k 
Crystal structure of the bacterial ribosome from escherichia coli in complex with paromomycin and ribosome recycling factor (rrf). this file contains the 30s subunit of the first 70s ribosome, with paromomycin bound. the entire crystal structure contains two 70s ribosomes and is described in remark 400. 2z4m 
Crystal structure of the bacterial ribosome from escherichia coli in complex with paromomycin and ribosome recycling factor (rrf). this file contains the 30s subunit of the second 70s ribosome, with paromomycin bound. the entire crystal structure contains two 70s ribosomes and is described in remark 400. 2zkq 
Structure of a mammalian ribosomal 40s subunit within an 80s complex obtained by docking homology models of the rna and proteins into an 8.7 a cryo-em map 2zm6 
Crystal structure of the thermus thermophilus 30s ribosomal subunit 3cdi 
Crystal structure of e. coli pnpase 3d5a 
Structural basis for translation termination on the 70s ribosome. this file contains the 30s subunit, release factor 1 (rf1), two trna, and mrna molecules of one 70s ribosome. the entire crystal structure contains two 70s ribosomes as described in remark 400. 3d5c 
Structural basis for translation termination on the 70s ribosome. this file contains the 30s subunit, release factor 1 (rf1), two trna, and mrna molecules of the second 70s ribosome. the entire crystal structure contains two 70s ribosomes as described in remark 400. 3df1 
Crystal structure of the bacterial ribosome from escherichia coli in complex with hygromycin b. this file contains the 30s subunit of the first 70s ribosome, with hygromycin b bound. the entire crystal structure contains two 70s ribosomes. 3df3 
Crystal structure of the bacterial ribosome from escherichia coli in complex with hygromycin b. this file contains the 30s subunit of the second 70s ribosome, with hygromycin b bound. the entire crystal structure contains two 70s ribosomes. 3e1a 
Structure of the 30s subunit and the trnas of e. coli ribosome in pre-accommodation state 3e1c 
Structure of the 30s subunit and the trnas of e. coli ribosome in post-accommodation state 3f1e 
Crystal structure of a translation termination complex formed with release factor rf2. this file contains the 30s subunit, rf2, two trna, and mrna molecules of one 70s ribosome. the entire crystal structure contains two 70s ribosomes as described in remark 400. 3f1g 
Crystal structure of a translation termination complex formed with release factor rf2. this file contains the 30s subunit, rf2, two trna, and mrna molecules of the second 70s ribosome. the entire crystal structure contains two 70s ribosomes as described in remark 400. 3fic 
T. thermophilus 70s ribosome in complex with mrna, trnas and ef-tu.gdp.kirromycin ternary complex, fitted to a 6.4 a cryo-em map. this file contains the 30s subunit and the ligands 3fih 
Ternary complex-bound e.coli 70s ribosome. this entry consists of the 30s subunit, trnas and the ternary complex. 3gku 
Crystal structure of a probable rna-binding protein from clostridium symbiosum atcc 14940 3huw 
Structure of ef-p bound to the 70s ribosome; this file contains the 30s subunit, mrna, p-site trna and ef-p for molecule i. 3huy 
Structure of ef-p bound to the 70s ribosome; this file contains the 30s subunit, mrna, p-site trna and ef-p for molecule ii. 3i1m 
Crystal structure of the e. coli 70s ribosome in an intermediate state of ratcheting 3i1o 
Crystal structure of the e. coli 70s ribosome in an intermediate state of ratcheting 3i1q 
Crystal structure of the e. coli 70s ribosome in an intermediate state of ratcheting 3i1s 
Crystal structure of the e. coli 70s ribosome in an intermediate state of ratcheting 3i1z 
Crystal structure of the e. coli 70s ribosome in an intermediate state of ratcheting 3i21 
Crystal structure of the e. coli 70s ribosome in an intermediate state of ratcheting - Links (links to other resources describing this domain)
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PFAM KH-domain INTERPRO IPR004087