Letunic et al. (2017) Nucleic Acids Res doi: 10.1093/nar/gkx922
Letunic et al. (2020) Nucleic Acids Res doi: 10.1093/nar/gkaa937

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MR_MLE Mandelate racemase / muconate lactonizing enzyme, C-terminal domain

SMART accession number:	SM00922
Description:	Mandelate racemase (MR) and muconate lactonizing enzyme (MLE) are two bacterial enzymes involved in aromatic acid catabolism. They catalyze mechanistically distinct reactions yet they are related at the level of their primary, quaternary (homooctamer) and tertiary structures (PUBMED:2215699), (PUBMED:8256284). This entry represents the C-terminal region of these proteins.
Interpro abstract (IPR013342):	Mandelate racemase EC 5.1.2.2 (MR) and muconate lactonising enzyme EC 5.5.1.1 (MLE) are two bacterial enzymes involved in aromatic acid catabolism. They catalyse mechanistically distinct reactions yet they are related at the level of their primary, quaternary (homooctamer) and tertiary structures [ (PUBMED:2215699) (PUBMED:8256284) ]. A number of other proteins also seem to be evolutionary related to these two enzymes. These include, various plasmid-encoded chloromuconate cycloisomerases EC 5.5.1.7 Escherichia coli protein rspA [ (PUBMED:7545940) ], E. coli bifunctional DGOA protein, a number of D-galactonate dehydratases and D-mannonate dehydratases, and a hypothetical protein from Streptomyces ambofaciens [ (PUBMED:8277241) ]. This entry represents the C-terminal region of these proteins.
Family alignment:	View or CHROMA formatHMM/Web logoCLUSTALW formatMSF formatFASTA formatPIR format

There are 58843 MR_MLE domains in 58841 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Taxonomic distribution of proteins containing MR_MLE domain.

  This tree includes only several representative species. The complete taxonomic breakdown of all proteins with MR_MLE domain is also avaliable.

  Click on the protein counts, or double click on taxonomic names to display all proteins containing MR_MLE domain in the selected taxonomic class.

• Cellular role (predicted cellular role)

• Cellular role: metabolism
  

• Literature (relevant references for this domain)

• Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.

  • Petsko GA, Kenyon GL, Gerlt JA, Ringe D, Kozarich JW
  • On the origin of enzymatic species.
  • Trends Biochem Sci. 1993; 18: 372-6
  • Display abstract

  • The diversity of enzyme catalytic function is remarkable, particularlywhen one considers that ancestral life forms must have started with a muchsmaller ensemble of proteins. In this article, we discuss the evolution ofthe mandelate pathway in pseudomonads as an example of how catalyticdiversity may have evolved. We suggest that existing enzymes that catalysethe chemistry needed to accomplish a transformation were recruited,followed by the evolution of specific binding.

  • Neidhart DJ, Kenyon GL, Gerlt JA, Petsko GA
  • Mandelate racemase and muconate lactonizing enzyme are mechanisticallydistinct and structurally homologous.
  • Nature. 1990; 347: 692-4
  • Display abstract

  • Mandelate racemase (MR) and muconate lactonizing enzyme (MLE) catalyseseparate and mechanistically distinct reactions necessary for thecatabolism of aromatic acids by Pseudomonas putida. The X-ray crystalstructure of MR, solved at 2.5 A resolution, reveals that the secondary,tertiary and quaternary structures of MR and MLE are remarkably similar;also, MR and MLE are about 26% identical in primary structure. However, MRhas no detectable MLE activity and vice versa. Thus, MR and MLE constitutethe first example of enzymes that catalyse different reactions, as opposedto mechanistically identical reactions on different substrates, yetpossess sufficient structural and sequence identity that they are likelyto have evolved from a common ancestor. The discovery that MR and MLEcatalyse different reactions but share a common structural framework hasbroad implications for the natural evolution of enzymes and metabolicpathways, as well as for the rational modification of enzyme activities.

  • Goldman A, Ollis DL, Steitz TA
  • Crystal structure of muconate lactonizing enzyme at 3 A resolution.
  • J Mol Biol. 1987; 194: 143-53
  • Display abstract

  • The crystal structure of muconate lactonizing enzyme has been solved at 3A resolution, and an unambiguous alpha-carbon backbone chain trace made.The enzyme contains three domains; the central domain is aparallel-stranded alpha-beta barrel, which has previously been reported insix other enzymes, including triose phosphate isomerase and pyruvatekinase. One novel feature of this enzyme is that its alpha-beta barrel hasonly seven parallel alpha-helices around the central core of eightparallel beta-strands; all other known alpha-beta barrels contain eightsuch helices. The N-terminal (alpha + beta) and C-terminal domains coverthe cleft where the eighth helix would be. The active site of muconatelactonizing enzyme has been found by locating the manganese ion that isessential for catalytic activity, and by binding and locating aninhibitor, alpha-ketoglutarate. The active site lies in a cleft betweenthe N-terminal and barrel domains; when the active sites of muconatelactonizing enzyme and triose phosphate isomerase are superimposed,barrel-strand 1 of triose phosphate isomerase is aligned withbarrel-strand 3 of muconate lactonizing enzyme. This implies thatstructurally homologous active-site residues in the two enzymes arecarried on different parts of the primary sequence; the ancestral genewould had to have been transposed during its evolution to the modernproteins, which seems unlikely. Therefore, these two enzymes may berelated by convergent, rather than divergent, evolution.

• Structure (3D structures containing this domain)

• 3D Structures of MR_MLE domains in PDB

  PDB code	Main view	Title
  1bkh		MUCONATE LACTONIZING ENZYME FROM PSEUDOMONAS PUTIDA
  1bqg		THE STRUCTURE OF THE D-GLUCARATE DEHYDRATASE PROTEIN FROM PSEUDOMONAS PUTIDA
  1dtn		MANDELATE RACEMASE MUTANT D270N CO-CRYSTALLIZED WITH (S)-ATROLACTATE
  1ec7		E. COLI GLUCARATE DEHYDRATASE NATIVE ENZYME
  1ec8		E. COLI GLUCARATE DEHYDRATASE BOUND TO PRODUCT 2,3-DIHYDROXY-5-OXO-HEXANEDIOATE
  1ec9		E. COLI GLUCARATE DEHYDRATASE BOUND TO XYLAROHYDROXAMATE
  1ecq		E. COLI GLUCARATE DEHYDRATASE BOUND TO 4-DEOXYGLUCARATE
  1f9c		CRYSTAL STRUCTURE OF MLE D178N VARIANT
  1fhu		CRYSTAL STRUCTURE ANALYSIS OF O-SUCCINYLBENZOATE SYNTHASE FROM E. COLI
  1fhv		CRYSTAL STRUCTURE ANALYSIS OF O-SUCCINYLBENZOATE SYNTHASE FROM E. COLI COMPLEXED WITH MG AND OSB
  1jct		Glucarate Dehydratase, N341L mutant Orthorhombic Form
  1jdf		Glucarate Dehydratase from E.coli N341D mutant
  1jpd		L-Ala-D/L-Glu Epimerase
  1jpm		L-Ala-D/L-Glu Epimerase
  1mdl		MANDELATE RACEMASE MUTANT K166R CO-CRYSTALLIZED WITH (R)-MANDELATE
  1mdr		THE ROLE OF LYSINE 166 IN THE MECHANISM OF MANDELATE RACEMASE FROM PSEUDOMONAS PUTIDA: MECHANISTIC AND CRYSTALLOGRAPHIC EVIDENCE FOR STEREOSPECIFIC ALKYLATION BY (R)-ALPHA-PHENYLGLYCIDATE
  1mns		ON THE ROLE OF LYSINE 166 IN THE MECHANISM OF MANDELATE RACEMASE FROM PSEUDOMONAS PUTIDA: MECHANISTIC AND CRYSTALLOGRAPHIC EVIDENCE FOR STEREOSPECIFIC ALKYLATION BY (R)-ALPHA-PHENYLGLYCIDATE
  1mra		MANDELATE RACEMASE MUTANT D270N CO-CRYSTALLIZED WITH (S)-ATROLACTATE
  1muc		STRUCTURE OF MUCONATE LACTONIZING ENZYME AT 1.85 ANGSTROMS RESOLUTION
  1nu5		Crystal structure of Pseudomonas sp. P51 Chloromuconate lactonizing enzyme
  1r0m		Structure of Deinococcus radiodurans N-acylamino acid racemase at 1.3 : insights into a flexible binding pocket and evolution of enzymatic activity
  1r6w		Crystal structure of the K133R mutant of o-Succinylbenzoate synthase (OSBS) from Escherichia coli. Complex with SHCHC
  1rvk		Crystal structure of enolase AGR_L_2751 from Agrobacterium Tumefaciens
  1sja		X-ray structure of o-Succinylbenzoate Synthase complexed with N-acetylmethionine
  1sjb		X-ray structure of o-succinylbenzoate synthase complexed with o-succinylbenzoic acid
  1sjc		x-ray structure of o-succinylbenzoate synthase complexed with N-succinyl methionine
  1sjd		x-ray structure of o-succinylbenzoate synthase complexed with n-succinyl phenylglycine
  1tkk		The Structure of a Substrate-Liganded Complex of the L-Ala-D/L-Glu Epimerase from Bacillus subtilis
  1tzz		Crystal structure of the protein L1841, unknown member of enolase superfamily from Bradyrhizobium japonicum
  1wue		Crystal structure of protein GI:29375081, unknown member of enolase superfamily from enterococcus faecalis V583
  1wuf		Crystal structure of protein GI:16801725, member of Enolase superfamily from Listeria innocua Clip11262
  1xpy		Structural Basis for Catalytic Racemization and Substrate Specificity of an N-Acylamino Acid Racemase Homologue from Deinococcus radiodurans
  1xs2		Structural Basis for Catalytic Racemization and Substrate Specificity of an N-Acylamino Acid Racemase Homologue from Deinococcus radiodurans
  1yey		Crystal Structure of L-fuconate Dehydratase from Xanthomonas campestris pv. campestris str. ATCC 33913
  2chr		A RE-EVALUATION OF THE CRYSTAL STRUCTURE OF CHLOROMUCONATE CYCLOISOMERASE
  2dw6		Crystal structure of the mutant K184A of D-Tartrate Dehydratase from Bradyrhizobium japonicum complexed with Mg++ and D-tartrate
  2dw7		Crystal structure of D-tartrate dehydratase from Bradyrhizobium japonicum complexed with Mg++ and meso-tartrate
  2fkp		The mutant G127C-T313C of Deinococcus Radiodurans N-acylamino acid racemase
  2gdq		Crystal structure of mandelate racemase/muconate lactonizing enzyme from Bacillus subtilis at 1.8 A resolution
  2gge		Crystal Structure of Mandelate Racemase/Muconate Lactonizing Enzyme from Bacillus Subtilis complexed with MG++ at 1.8 A
  2ggg		The mutant A68C-D72C of Deinococcus Radiodurans N-acylamino acid racemase
  2ggh		The mutant A68C-D72C-NLQ of Deinococcus Radiodurans Nacylamino acid racemase
  2ggi		The mutant E149C-A182C of Deinococcus Radiodurans N-acylamino acid racemase
  2ggj		The mutant Y218C of Deinococcus Radiodurans N-acylamino acid racemase
  2gl5		Crystal Structure of Putative Dehydratase from Salmonella Thyphimurium
  2gsh		Crystal structure of L-rhamnonate dehydratase from Salmonella typhimurium
  2hne		Crystal structure of l-fuconate dehydratase from xanthomonas campestris pv. campestris str. ATCC 33913
  2hxt		Crystal structure of L-Fuconate Dehydratase from Xanthomonas campestris liganded with Mg++ and D-erythronohydroxamate
  2hxu		Crystal structure of K220A mutant of L-Fuconate Dehydratase from Xanthomonas campestris liganded with Mg++ and L-fuconate
  2hzg		Crystal stucture of predicted Mandelate racemase from Rhodobacter sphaeroides
  2i5q		Crystal structure of Apo L-rhamnonate dehydratase from Escherichia Coli
  2mnr		MECHANISM OF THE REACTION CATALYZED BY MANDELATE RACEMASE. 2. CRYSTAL STRUCTURE OF MANDELATE RACEMASE AT 2.5 ANGSTROMS RESOLUTION: IDENTIFICATION OF THE ACTIVE SITE AND POSSIBLE CATALYTIC RESIDUES
  2muc		MUCONATE CYCLOISOMERASE VARIANT F329I
  2nql		Crystal structure of a member of the enolase superfamily from Agrobacterium tumefaciens
  2o56		Crystal Structure of a Member of the Enolase Superfamily from Salmonella Typhimurium
  2ofj		Crystal structure of the E190A mutant of o-succinylbenzoate synthase from Escherichia coli
  2og9		Crystal Structure of mandelate racemase/muconate lactonizing enzyme from Polaromonas sp. JS666
  2oo6		Crystal structure of putative L-alanine-DL-glutamate epimerase from Burkholderia xenovorans strain LB400
  2opj		Crystal structure of O-succinylbenzoate synthase
  2oqh		Crystal structure of an isomerase from Streptomyces coelicolor A3(2)
  2oqy		The crystal structure of muconate cycloisomerase from Oceanobacillus iheyensis
  2ovl		Crystal structure of a racemase from Streptomyces coelicolor A3(2)
  2ox4		Crystal structure of putative dehydratase from Zymomonas mobilis ZM4
  2oz3		Crystal structure of L-Rhamnonate dehydratase from Azotobacter vinelandii
  2oz8		Crystal structure of putative mandelate racemase from Mesorhizobium loti
  2ozt		Crystal structure of O-succinylbenzoate synthase from Thermosynechococcus elongatus BP-1
  2p0i		Crystal structure of L-rhamnonate dehydratase from Gibberella zeae
  2p3z		Crystal structure of L-Rhamnonate dehydratase from Salmonella typhimurium
  2p88		Crystal structure of N-succinyl Arg/Lys racemase from Bacillus cereus ATCC 14579
  2p8b		Crystal structure of N-succinyl Arg/Lys racemase from Bacillus cereus ATCC 14579 complexed with N-succinyl Lys.
  2p8c		Crystal structure of N-succinyl Arg/Lys racemase from Bacillus cereus ATCC 14579 complexed with N-succinyl Arg.
  2pge		Crystal structure of MenC from Desulfotalea psychrophila LSv54
  2pgw		Crystal structure of a putative muconate cycloisomerase from Sinorhizobium meliloti 1021
  2pmq		Crystal structure of a mandelate racemase/muconate lactonizing enzyme from Roseovarius sp. HTCC2601
  2pod		Crystal structure of a member of enolase superfamily from Burkholderia pseudomallei K96243
  2poz		Crystal structure of a putative dehydratase from Mesorhizobium loti
  2pp0		Crystal structure of L-talarate/galactarate dehydratase from Salmonella typhimurium LT2
  2pp1		Crystal structure of L-talarate/galactarate dehydratase from Salmonella typhimurium LT2 liganded with Mg and L-lyxarohydroxamate
  2pp3		Crystal structure of L-talarate/galactarate dehydratase mutant K197A liganded with Mg and L-glucarate
  2ppg		Crystal structure of putative isomerase from Sinorhizobium meliloti
  2ps2		Crystal structure of putative mandelate racemase/muconate lactonizing enzyme from Aspergillus oryzae
  2qdd		Crystal structure of a member of enolase superfamily from Roseovarius nubinhibens ISM
  2qde		Crystal structure of mandelate racemase/muconate lactonizing family protein from Azoarcus sp. EbN1
  2qjj		Crystal structure of D-Mannonate dehydratase from Novosphingobium aromaticivorans
  2qjm		Crystal structure of the K271E mutant of Mannonate dehydratase from Novosphingobium aromaticivorans complexed with Mg and D-mannonate
  2qjn		Crystal structure of D-mannonate dehydratase from Novosphingobium aromaticivorans complexed with Mg and 2-keto-3-deoxy-D-gluconate
  2qq6		Crystal structure of mandelate racemase/muconate lactonizing enzyme-like protein from Rubrobacter xylanophilus DSM 9941
  2qvh		Crystal structure of O-succinylbenzoate synthase complexed with O-succinyl benzoate (OSB)
  2rdx		Crystal structure of mandelate racemase/muconate lactonizing enzyme from Roseovarius nubinhibens ISM
  2zad		Crystal Structure of Muconate Cycloisomerase from Thermotoga maritima MSB8
  2zc8		Crystal structure of N-Acylamino Acid Racemase from Thermus thermophilus HB8
  3bjs		Crystal structure of a member of enolase superfamily from Polaromonas sp. JS666
  3box		Crystal structure of L-rhamnonate dehydratase from Salmonella typhimurium complexed with Mg
  3bsm		Crystal structure of D-mannonate dehydratase from Chromohalobacter salexigens
  3cb3		Crystal structure of L-Talarate dehydratase from Polaromonas sp. JS666 complexed with Mg and L-glucarate
  3ck5		Crystal structure of a racemase from Streptomyces coelicolor A3(2) with bound magnesium
  3ct2		Crystal structure of muconate cycloisomerase from Pseudomonas fluorescens
  3cxo		Crystal structure of L-rhamnonate dehydratase from Salmonella typhimurium complexed with Mg and 3-deoxy-L-rhamnonate
  3cyj		Crystal structure of a mandelate racemase/muconate lactonizing enzyme-like protein from Rubrobacter xylanophilus
  3d46		Crystal structure of L-rhamnonate dehydratase from Salmonella typhimurium complexed with Mg and L-tartrate
  3d47		Crystal structure of L-rhamnonate dehydratase from Salmonella typhimurium complexed with Mg and D-malate
  3ddm		CRYSTAL STRUCTURE OF MANDELATE RACEMASE/MUCONATE LACTONIZING ENZYME FROM Bordetella bronchiseptica RB50
  3deq		Crystal structure of dipeptide epimerase from Thermotoga maritima complexed with L-Ala-L-Leu dipeptide
  3der		Crystal structure of dipeptide epimerase from Thermotoga maritima complexed with L-Ala-L-Lys dipeptide
  3des		Crystal structure of dipeptide epimerase from Thermotoga maritima complexed with L-Ala-L-Phe dipeptide
  3dfh		crystal structure of putative mandelate racemase / muconate lactonizing enzyme from Vibrionales bacterium SWAT-3
  3dfy		Crystal structure of apo dipeptide epimerase from Thermotoga maritima
  3dg3		Crystal structure of muconate lactonizing enzyme from Mucobacterium Smegmatis
  3dg6		Crystal structure of muconate lactonizing enzyme from Mucobacterium Smegmatis complexed with muconolactone
  3dg7		Crystal structure of muconate lactonizing enzyme from Mucobacterium Smegmatis complexed with muconolactone
  3dgb		Crystal structure of muconate lactonizing enzyme from Pseudomonas Fluorescens complexed with muconolactone
  3dip		Crystal structure of an enolase protein from the environmental genome shotgun sequencing of the Sargasso Sea
  3eez		Crystal structure of a putative mandelate racemase/muconate lactonizing enzyme from Silicibacter pomeroyi
  3ekg		CRYSTAL STRUCTURE OF L-RHAMNONATE DEHYDRATASE FROM AZOTOBACTER VINELANDII complexed with Mg and L-TARTRATE
  3es7		Crystal structure of divergent enolase from Oceanobacillus Iheyensis complexed with Mg and L-malate.
  3es8		Crystal structure of divergent enolase from Oceanobacillus Iheyensis complexed with Mg and L-malate.
  3fcp		Crystal structure of Muconate lactonizing enzyme from Klebsiella pneumoniae
  3fj4		Crystal structure of muconate lactonizing enzyme from Pseudomonas Fluorescens complexed with muconolactone
  3fvd		Crystal structure of a member of enolase superfamily from ROSEOVARIUS NUBINHIBENS ISM complexed with magnesium
  3fxg		Crystal structure of rhamnonate dehydratase from Gibberella zeae complexed with Mg
  3fyy		Crystal structure of divergent enolase from Oceanobacillus iheyensis complexed with Mg
  3gc2		1.85 Angstrom Crystal Structure of O-succinylbenzoate Synthase from Salmonella typhimurium in Complex with Succinic Acid
  3gd6		Crystal structure of divergent enolase from Oceanobacillus iheyensis complexed with phosphate
  3go2		Crystal structure of putative L-alanine-DL-glutamate epimerase from Burkholderia xenovorans strain LB400 bound to magnesium
  3gy1		CRYSTAL STRUCTURE OF putative mandelate racemase/muconate lactonizing protein from Clostridium beijerinckii NCIMB 8052
  3h12		Crystal structure of putative mandelate racemase from Bordetella Bronchiseptica RB50
  3h7v		CRYSTAL STRUCTURE OF O-SUCCINYLBENZOATE SYNTHASE FROM THERMOSYNECHOCOCCUS ELONGATUS BP-1 complexed with MG in the active site
  3hpf		Crystal structure of the mutant Y90F of divergent galactarate dehydratase from Oceanobacillus iheyensis complexed with Mg and galactarate
  3i4k		Crystal structure of Muconate lactonizing enzyme from Corynebacterium glutamicum
  3i6e		CRYSTAL STRUCTURE OF MUCONATE LACTONIZING ENZYME FROM Ruegeria pomeroyi.
  3i6t		Crystal structure of muconate cycloisomerase from Jannaschia sp.
  3iji		Structure of dipeptide epimerase from Bacteroides thetaiotaomicron complexed with L-Ala-D-Glu; nonproductive substrate binding.
  3ijl		Structure of dipeptide epimerase from Bacteroides thetaiotaomicron complexed with L-Pro-D-Glu; nonproductive substrate binding.
  3ijq		Structure of dipeptide epimerase from Bacteroides thetaiotaomicron complexed with L-Ala-D-Glu; productive substrate binding.
  3ik4		CRYSTAL STRUCTURE OF mandelate racemase/muconate lactonizing protein from Herpetosiphon aurantiacus
  3jva		Crystal structure of Dipeptide Epimerase from Enterococcus faecalis V583
  3jw7		Crystal structure of Dipeptide Epimerase from Enterococcus faecalis V583 complexed with Mg and dipeptide L-Ile-L-Tyr
  3jzu		Crystal structure of Dipeptide Epimerase from Enterococcus faecalis V583 complexed with Mg and dipeptide L-Leu-L-Tyr
  3k1g		Crystal structure of Dipeptide Epimerase from Enterococcus faecalis V583 complexed with Mg and dipeptide L-Ser-L-Tyr
  3kum		Crystal structure of Dipeptide Epimerase from Enterococcus faecalis V583 complexed with Mg and dipeptide L-Arg-L-Tyr
  3mqt		Crystal structure of a mandelate racemase/muconate lactonizing enzyme from Shewanella pealeana
  3msy		Crystal Structure of Mandelate racemase/muconate lactonizing enzyme from a Marine actinobacterium
  3muc		MUCONATE CYCLOISOMERASE VARIANT I54V
  3mwc		Crystal structure of probable o-succinylbenzoic acid synthetase from kosmotoga olearia
  3my9		Crystal structure of a muconate cycloisomerase from Azorhizobium caulinodans
  3mzn		Crystal structure of probable glucarate dehydratase from chromohalobacter salexigens dsm 3043
  3n4e		CRYSTAL STRUCTURE OF mandelate racemase/muconate lactonizing protein from Paracoccus denitrificans Pd1222
  3n4f		CRYSTAL STRUCTURE OF Mandelate racemase/muconate lactonizing protein from Geobacillus sp. Y412MC10
  3n6h		Crystal structure of Mandelate racemase/muconate lactonizing protein from Actinobacillus succinogenes 130Z complexed with magnesium/sulfate
  3n6j		Crystal structure of Mandelate racemase/muconate lactonizing protein from Actinobacillus succinogenes 130Z
  3nfu		Crystal structure of probable glucarate dehydratase from chromohalobacter salexigens dsm 3043 complexed with magnesium
  3no1		Crystal Structure of Mandelate racemase/muconate lactonizing enzyme from a Marine actinobacterium in complex with magnesium
  3nxl		Crystal structure of Glucarate dehydratase from Burkholderia cepacia complexed with magnesium
  3op2		Crystal structure of putative mandelate racemase from Bordetella bronchiseptica RB50 complexed with 2-oxoglutarate/phosphate
  3ops		Crystal structure of mandelate racemase/muconate lactonizing protein FROM GEOBACILLUS SP. Y412MC10 complexed with magnesium/tartrate
  3ow1		Crystal structure of D-mannonate dehydratase from Chromohalobacter salexigens complexed with MG
  3ozm		Crystal structure of enolase superfamily member from Bordetella bronchiseptica complexed with Mg, m-Xylarate and L-Lyxarate
  3ozy		Crystal structure of enolase superfamily member from Bordetella bronchiseptica complexed with Mg and m-Xylarate
  3p0w		Crystal structure of D-Glucarate dehydratase from Ralstonia solanacearum complexed with Mg and D-glucarate
  3p3b		CRYSTAL STRUCTURE OF Galacturonate DEHYDRATASE FROM GEOBACILLUS SP. COMPLEXED WITH D-TARTRATE
  3p93		Crystal structure of D-mannonate dehydratase from Chromohalobacter Salexigens complexed with MG,D-Mannonate and 2-keto-3-deoxy-D-Gluconate
  3pfr		Crystal structure of D-Glucarate dehydratase related protein from Actinobacillus Succinogenes complexed with D-Glucarate
  3pk7		Crystal structure of D-mannonate dehydratase from Chromohalobacter salexigens with MG and Glycerol bound in the active site
  3pwg		Crystal structure of the mutant S29G.P34A of D-Glucarate dehydratase from Escherichia coli complexed with product 5-keto-4-deoxy-D-Glucarate
  3pwi		Crystal structure of the mutant P34A of D-Glucarate dehydratase from Escherichia coli complexed with product 5-keto-4-deoxy-D-Glucarate
  3q45		Crystal structure of Dipeptide Epimerase from Cytophaga hutchinsonii complexed with Mg and dipeptide D-Ala-L-Val
  3q4d		Crystal structure of dipeptide epimerase from Cytophaga hutchinsonii complexed with Mg and dipeptide D-Ala-L-Ala
  3qke		Crystal structure of D-mannonate dehydratase from Chromohalobacter Salexigens complexed with Mg and D-Gluconate
  3qld		Structure of Probable Mandelate Racemase (AaLAA1DRAFT_2112) from Alicyclobacillus Acidocaldarius
  3qpe		Crystal structure of Galacturonate Dehydratase from GEOBACILLUS SP. complexed with D-Galacturonate and 5-keto-4-deoxy-D-Galacturonate
  3r0k		Crystal structure of NYSGRC enolase target 200555, a putative dipeptide epimerase from Francisella philomiragia : Tartrate bound, no Mg
  3r0u		Crystal structure of NYSGRC enolase target 200555, a putative dipeptide epimerase from Francisella philomiragia : Tartrate and Mg complex
  3r10		Crystal structure of NYSGRC enolase target 200555, a putative dipeptide epimerase from Francisella philomiragia : Mg complex
  3r11		Crystal structure of NYSGRC enolase target 200555, a putative dipeptide epimerase from Francisella philomiragia : Mg and Fumarate complex
  3r1z		Crystal structure of NYSGRC enolase target 200555, a putative dipeptide epimerase from Francisella philomiragia : Complex with L-Ala-L-Glu and L-Ala-D-Glu
  3r25		Crystal structure of enolase superfamily member from Vibrionales bacterium complexed with Mg and Glycerol in the active site
  3rcy		CRYSTAL STRUCTURE OF Mandelate racemase/muconate lactonizing enzyme-like protein from Roseovarius sp. TM1035
  3rgt		Crystal structure of d-mannonate dehydratase from Chromohalobacter salexigens complexed with D-Arabinohydroxamate
  3rit		Crystal structure of Dipeptide Epimerase from Methylococcus capsulatus complexed with Mg and dipeptide L-Arg-D-Lys
  3ro6		Crystal structure of Dipeptide Epimerase from Methylococcus capsulatus complexed with Mg ion
  3rr1		Crystal structure of enolase PRK14017 (target EFI-500653) from Ralstonia pickettii 12J
  3rra		Crystal structure of enolase PRK14017 (target EFI-500653) from Ralstonia pickettii 12J with magnesium bound
  3s47		Crystal structure of enolase superfamily member from Clostridium beijerincki complexed with Mg
  3s5s		Crystal structure of putative mandelate racemase/muconate lactonizing enzyme (PSI/COM target 200551) from Sorangium cellulosum
  3sbf		Crystal structure of the mutant P311A of enolase superfamily member from VIBRIONALES BACTERIUM complexed with Mg and D-Arabinonate
  3sjn		Crystal structure of enolase Spea_3858 (target EFI-500646) from Shewanella pealeana with magnesium bound
  3sn0		Crystal structure of putative L-alanine-DL-glutamate epimerase from Burkholderia xenovorans strain LB400 bound to magnesium and fumarate
  3sn1		Crystal structure of putative L-alanine-DL-glutamate epimerase from Burkholderia xenovorans strain LB400 bound to magnesium and tartrate
  3sn4		Crystal structure of putative L-alanine-DL-glutamate epimerase from Burkholderia xenovorans strain LB400 bound to magnesium and alpha-ketoglutarate
  3sqs		Crystal Structure of a putative mandelate racemase/muconate lactonizing protein from Dinoroseobacter shibae DFL 12
  3ssz		The crystal structure of Mandelate racemase/muconate lactonizing enzyme from Rhodobacteraceae bacterium
  3stp		Crystal structure of a putative galactonate dehydratase
  3t4w		The crystal structure of mandelate racemase/muconate lactonizing enzyme from Sulfitobacter sp
  3t6c		Crystal structure of an enolase from pantoea ananatis (efi target efi-501676) with bound d-gluconate and mg
  3t8q		Crystal structure of mandelate racemase/muconate lactonizing enzyme family protein from Hoeflea phototrophica
  3t9p		Crystal structure of a putative mandelate racemase/muconate lactonizing enzyme family protein from Roseovarius
  3tcs		Crystal structure of a putative racemase from Roseobacter denitrificans
  3thu		Crystal structure of an enolase from sphingomonas sp. ska58 (efi target efi-501683) with bound mg
  3tj4		Crystal structure of an enolase from agrobacterium tumefaciens (efi target efi-502087) no mg
  3tji		CRYSTAL STRUCTURE OF AN ENOLASE FROM ENTEROBACTER sp. 638 (EFI TARGET EFI-501662) with BOUND MG
  3toy		CRYSTAL STRUCTURE OF ENOLASE BRADO_4202 (TARGET EFI-501651) FROM Bradyrhizobium sp. ORS278 WITH CALCIUM AND ACETATE BOUND
  3tte		Crystal structure of enolase brado_4202 (target EFI-501651) from Bradyrhizobium complexed with magnesium and mandelic acid
  3tw9		Crystal structure of gluconate dehydratase (TARGET EFI-501679) from Salmonella enterica subsp. enterica serovar Enteritidis str. P125109
  3twa		Crystal structure of gluconate dehydratase (TARGET EFI-501679) from Salmonella enterica subsp. enterica serovar Enteritidis str. P125109 complexed with magnesium and glycerol
  3twb		Crystal structure of gluconate dehydratase (TARGET EFI-501679) from Salmonella enterica subsp. enterica serovar Enteritidis str. P125109 complexed with magnesium and gluconic acid
  3u4f		Crystal structure of a mandelate racemase (muconate lactonizing enzyme family protein) from Roseovarius nubinhibens
  3u9i		The crystal structure of Mandelate racemase/muconate lactonizing enzyme from Roseiflexus sp.
  3ugv		Crystal structure of an enolase from alpha pretobacterium bal199 (EFI TARGET EFI-501650) with bound MG
  3uxk		P. putida mandelate racemase co-crystallized with the intermediate analogue benzohydroxamate
  3uxl		P. putida mandelate racemase co-crystallized with the intermediate analogue cupferron
  3v3w		Crystal structure of an enolase from the soil bacterium Cellvibrio japonicus (TARGET EFI-502161) with bound MG and glycerol
  3v4b		Crystal structure of an enolase from the soil bacterium Cellvibrio japonicus (TARGET EFI-502161) with bound MG and L-tartrate
  3v5c		Crystal structure of the mutant E234A of Galacturonate Dehydratase from GEOBACILLUS SP. complexed with Mg
  3v5f		Crystal structure of the mutant E234A of Galacturonate Dehydratase from GEOBACILLUS SP. complexed with Mg
  3va8		Crystal structure of enolase FG03645.1 (target EFI-502278) from Gibberella zeae PH-1 complexed with magnesium, formate and sulfate
  3vc5		Crystal structure of enolase Tbis_1083(TARGET EFI-502310) FROM Thermobispora bispora DSM 43833 complexed with phosphate
  3vc6		Crystal structure of enolase Tbis_1083(TARGET EFI-502310) FROM Thermobispora bispora DSM 43833 complexed with magnesium and formate
  3vcc		CRYSTAL STRUCTURE OF D-Galacturonate Dehydratase from GEOBACILLUS SP. complexed with Mg
  3vcn		Crystal structure of mannonate dehydratase (target EFI-502209) from Caulobacter crescentus CB15
  3vdg		Crystal structure of enolase MSMEG_6132 (TARGET EFI-502282) from Mycobacterium smegmatis str. MC2 155 complexed with formate and acetate
  3vfc		Crystal structure of enolase MSMEG_6132 (TARGET EFI-502282) from Mycobacterium smegmatis str. MC2 155 complexed with tartrate
  4a35		Crystal structure of human Mitochondrial enolase superfamily member 1 (ENOSF1)
  4a6g		N-acyl amino acid racemase from Amycalotopsis sp. Ts-1-60: G291D- F323Y mutant in complex with N-acetyl methionine
  4dhg		Crystal structure of enolase TBIS_1083(TARGET EFI-502310) from Thermobispora bispora dsm 43833, an open loop conformation
  4dn1		Crystal structure of an ENOLASE (mandelate racemase subgroup member) from Agrobacterium tumefaciens (target EFI-502088) with bound mg and formate
  4dwd		Crystal structure of mandelate racemase/muconate lactonizing protein from Paracoccus denitrificans PD1222 complexed with magnesium
  4dx3		Crystal structure of an enolase (mandelate racemase subgroup, target EFI-502086) from Agrobacterium tumefaciens, with a succinimide residue
  4dxk		Crystal structure of an enolase (mandelate racemase subgroup, target EFI-502086) from Agrobacterium tumefaciens, with a succinimide residue, na and phosphate
  4dye		Crystal structure of an enolase (putative sugar isomerase, target efi-502095) from streptomyces coelicolor, no mg, ordered loop
  4e4f		Crystal structure of enolase PC1_0802 (TARGET EFI-502240) from Pectobacterium carotovorum subsp. carotovorum PC1
  4e4u		Crystal structure of a putative Mandelate racemase/Muconate lactonizing enzyme (Target PSI-200780) from Burkholderia SAR-1
  4e5t		Crystal structure of a putative Mandelate racemase/Muconate lactonizing enzyme (Target PSI-200750) from Labrenzia alexandrii DFL-11
  4e6m		Crystal structure of Putative dehydratase protein from Salmonella enterica subsp. enterica serovar Typhimurium (Salmonella typhimurium)
  4e8g		Crystal structure of an enolase (mandelate racemase subgroup) from paracococus denitrificans pd1222 (target nysgrc-012907) with bound mg
  4f4r		Crystal structure of D-mannonate dehydratase homolog from Chromohalobacter salexigens (Target EFI-502114), with bound NA, ordered loop
  4fi4		Crystal structure of mannonate dehydratase PRK15072 (TARGET EFI-502214) from Caulobacter sp. K31
  4fp1		P. putida mandelate racemase co-crystallized with 3,3,3-trifluoro-2-hydroxy-2-(trifluoromethyl) propionic acid
  4g8t		Crystal structure of a glucarate dehydratase related protein, from actinobacillus succinogenes, target EFI-502312, with sodium and sulfate bound, ordered loop
  4gfi		Crystal structure of EFI-502318, an enolase family member from Agrobacterium tumefaciens with homology to dipeptide epimerases (bound sodium, L-Ala-L-Glu with ordered loop)
  4ggb		Crystal structure of a proposed galactarolactone cycloisomerase from agrobacterium tumefaciens, TARGET EFI-500704, WITH BOUND CA, DISORDERED LOOPS
  4ggh		Crystal structure of an enolase family member from vibrio harveyi (efi-target 501692) with homology to mannonate dehydratase, with mg, hepes, and ethylene glycol bound (ordered loops, space group c2221)
  4gir		Crystal structure of an enolase family member from vibrio harveyi (efi-target 501692) with homology to mannonate dehydratase, with mg, ethylene glycol and sulfate bound (ordered loops, space group P41212)
  4gis		crystal structure of an enolase family member from vibrio harveyi (efi-target 501692) with homology to mannonate dehydratase, with mg, glycerol and dicarboxylates bound (mixed loops, space group I4122)
  4gme		Crystal structure of mannonate dehydratase (target EFI-502209) from caulobacter crescentus cb15 complexed with magnesium and d-mannonate
  4gyp		Crystal structure of the heterotetrameric complex of GlucD and GlucDRP from E. coli K-12 MG1655 (EFI TARGET EFI-506058)
  4h19		Crystal structure of an enolase (mandelate racemase subgroup, target EFI-502087) from agrobacterium tumefaciens, with bound Mg and d-ribonohydroxamate, ordered loop
  4h1z		Crystal structure of putative isomerase from Sinorhizobium meliloti, open loop conformation (target EFI-502104)
  4h2h		Crystal structure of an enolase (mandalate racemase subgroup, target EFI-502101) from Pelagibaca bermudensis htcc2601, with bound mg and l-4-hydroxyproline betaine (betonicine)
  4h83		Crystal structure of Mandelate racemase/muconate lactonizing enzyme (EFI target:502127)
  4hcd		Crystal structure of D-glucarate dehydratase from agrobacterium tumefaciens complexed with magnesium
  4hch		CRYSTAL STRUCTURE OF D-GLUCARATE DEHYDRATASE FROM AGROBACTERIUM TUMEFACIENS complexed with magnesium and L-tartrate
  4hcl		CRYSTAL STRUCTURE OF D-GLUCARATE DEHYDRATASE FROM AGROBACTERIUM TUMEFACIENS complexed with magnesium and L-Lyxarohydroxamate
  4hn8		Crystal structure of a putative D-glucarate dehydratase from Pseudomonas mendocina ymp
  4hnc		P. putida C92S/K166C/C264S mandelate racemase co-crystallized with benzilic acid
  4hnl		Crystal structure of ENOLASE EGBG_01401 (TARGET EFI-502226) from Enterococcus gallinarum EG2
  4hpn		Crystal structure of a proposed galactarolactone cycloisomerase from Agrobacterium Tumefaciens, target EFI-500704, with bound Ca, ordered loops
  4hyr		Structure of putative Glucarate dehydratase from Acidaminococcus sp. D21 with unusual static disorder
  4ihc		Crystal structure of probable mannonate dehydratase Dd703_0947 (target EFI-502222) from Dickeya dadantii Ech703
  4il0		Crystal structure of GlucDRP from E. coli K-12 MG1655 (EFI target EFI-506058)
  4il2		Crystal structure of D-mannonate dehydratase (rspA) from E. coli CFT073 (EFI TARGET EFI-501585)
  4ip4		Crystal structure of l-fuconate dehydratase from Silicibacter sp. tm1040 liganded with Mg
  4ip5		Crystal structure of l-fuconate dehydratase from Silicibacter sp. tm1040 liganded with Mg and d-erythronohydroxamate
  4it1		Crystal structure of enolase pfl01_3283 (target efi-502286) from pseudomonas fluorescens pf0-1 with bound magnesium, potassium and tartrate
  4izg		Crystal structure of an enolase (mandelate racemase subgroup) from paracococus denitrificans pd1222 (target nysgrc-012907) with bound cis-4oh-d-proline betaine (product)
  4j1o		Crystal structure of an enolase (mandelate racemase subgroup) from paracococus denitrificans pd1222 (target nysgrc-012907) with bound l-proline betaine (substrate)
  4j3z		Crystal structure of mandelate racemase/muconate lactonizing enzyme from Jannaschia sp. CCS1
  4jn7		CRYSTAL STRUCTURE OF AN ENOLASE (PUTATIVE GALACTARATE DEHYDRATASE, TARGET EFI-500740) FROM AGROBACTERIUM RADIOBACTER, BOUND NA and L-MALATE, ORDERED ACTIVE SITE
  4jn8		Crystal structure of an enolase (putative galactarate dehydratase, target efi-500740) from agrobacterium radiobacter, bound sulfate, no metal ion, ordered active site
  4k1w		Crystal structure of the A314P mutant of mannonate dehydratase from novosphingobium aromaticivorans complexed with mg and d-mannonate
  4k2s		Crystal structure of the mutant P317A of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg and d-gluconate
  4k8g		Crystal structure of D-Mannonate dehydratase from Novosphingobium aromaticivorans mutant (V161A, R163A, K165G, L166A, Y167G, Y168A, E169G)
  4kem		Crystal structure of a tartrate dehydratase from azospirillum, target efi-502395, with bound mg and a putative acrylate ion, ordered active site
  4kpl		Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with Mg,d-mannonate and 2-keto-3-deoxy-d-gluconate
  4kt2		Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg and glycerol
  4kws		4KWS
  4m0x		4M0X
  4m6u		P. putida mandelate racemase co-crystallized with tartronic acid
  4mgg		Crystal structure of an enolase (mandelate racemase subgroup) from labrenzia aggregata iam 12614 (target nysgrc-012903) with bound mg, space group p212121
  4mmw		Crystal structure of D-glucarate dehydratase from Agrobacterium tumefaciens complexed with magnesium, L-Xylarohydroxamate and L-Lyxarohydroxamate
  4x2p		4X2P
  5fjo		5FJO
  5fjp		5FJP
  5fjr		5FJR
  5fjt		5FJT
  5fju		5FJU

• Links (links to other resources describing this domain)

• PFAM	MR_MLE
  INTERPRO	IPR013342

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