PhBPInsect pheromone/odorant binding protein domains. |
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| SMART accession number: | SM00708 |
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| Description: | |
| Interpro abstract (IPR006625): | PhBP is the insect pheromone/odorant binding protein [(PUBMED:7612612)]. |
| GO function: | odorant binding (GO:0005549) |
| Family alignment: |
There are 780 PhBP domains in 746 proteins in SMART's nrdb database.
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- Evolution (species in which this domain is found)
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Go to specific node: Anopheles gambiae, Drosophila melanogaster - Literature (relevant references for this domain)
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Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.
- Du G, Prestwich GD
- Protein structure encodes the ligand binding specificity in pheromonebinding proteins.
- Biochemistry. 1995; 34: 8726-32
- Display abstract
The ligand specificities and binding affinities of three recombinantpheromone binding proteins (PBPs) of two saturniid moths (genus Antheraea)were determined by using a novel binding assay in conjunction with twotritium-labeled constituents of the pheromone blend,[3H]-6E,11Z-hexadecadienyl acetate and [3H]-4E,9Z-tetradecadienyl acetate.The new binding assay, in which nonspecific adsorption to a plastic vesselis suppressed by presaturation of the surface with a 1-alkanol, allowsmeasurement of dissociation constants (KD) for lipophilic ligands fortheir carrier proteins. The three PBPs showed KD values for[3H]-6E,11Z-16:Ac and [3H]-4E,9Z-14:Ac between 0.6 and 30 microM, asdetermined by Scatchard analysis. Importantly, two PBPs (Aper-1 andAper-2) from one species showed opposite binding specificities for thesetwo ligands. Aper-1, like Apol-3, showed 15-fold higher affinity for6E,11Z-16:Ac than for 4E,9Z-14:Ac, while Aper-2 showed a 3.5-foldpreference for binding the shorter chain compound. In addition, for theApol-3 PBP, displacement of [3H]-6E,11Z-16:Ac binding by other pheromonecomponents or analogs showed a clear trend in relative binding affinity:6E,11Z-16:Ac > 4E,9Z-14:Ac > 6E,11Z-16:Al approximately 16:Ac >6E,11Z-16:OH > 4E,9Z-14:OH. These data clearly demonstrate a > 1000-foldrange of binding affinities among these very similar structures andunambiguously demonstrate the specificity of the PBP-pheromoneinteraction. Moreover, this assay offers the potential for determiningligand specificities for odorant binding proteins and other proteins inthe vertebrate lipocalin superfamily.
- Prestwich GD
- Bacterial expression and photoaffinity labeling of a pheromone bindingprotein.
- Protein Sci. 1993; 2: 420-8
- Display abstract
The first high-level production of a binding-active odorant bindingprotein is described. The expression cassette polymerase chain reactionwas used to generate a DNA fragment encoding the pheromone binding protein(PBP) of the male moth Antheraea polyphemus. Transformation of Escherichiacoli cells with a vector containing this construct generated clones which,when induced with isopropyl beta-D-thiogalactopyranoside, produced the14-kDa PBP in both the soluble fraction and in inclusion bodies.Purification of the soluble recombinant PBP by preparative isoelectricfocusing and gel filtration gave > 95% homogeneous protein, which wasimmunoreactive with an anti-PBP antiserum and exhibited specific,pheromone-displaceable covalent modification by the photoaffinity label[3H]6E,11Z-hexadecadienyl diazoacetate. Recombinant PBP wasindistinguishable from the insect-derived PBP, as determined by bothnative and denaturing gel electrophoresis, immunoreactivity, andphotoaffinity labeling properties. Moreover, the insoluble inclusion bodyprotein could be solubilized, refolded, and purified by the sameprocedures to give a recombinant PBP indistinguishable from the solublePBP. Proton NMR spectra of the soluble and refolded protein providefurther evidence that they possess the same folded structure.
- Raming K, Krieger J, Breer H
- Molecular cloning of an insect pheromone-binding protein.
- FEBS Lett. 1989; 256: 215-8
- Display abstract
Clones coding for the pheromone binding protein precursor have beenselected from a cDNA library derived from antennae of the male moth,Antheraea polyphemus. The deduced protein sequence consists of a signalpeptide of 20 amino acid residues and a mature binding protein of 142amino acid residues. RNA blot hybridization indicated that the mRNA isselectively expressed in the antennae of the male moth.
- Structure (3D structures containing this domain)
3D Structures of PhBP domains in PDB
PDB code Main view Title 1c3y 
Thp12-carrier protein from yellow meal worm 1c3z 
Thp12-carrier protein from yellow meal worm 1dqe 
Bombyx mori pheromone binding protein 1gm0 
A form of the pheromone-binding protein from bombyx mori 1ls8 
Nmr structure of the unliganded bombyx mori pheromone- binding protein at physiological ph 1oof 
Complex of drosophila odorant binding protein lush with ethanol 1oog 
Complex of drosophila odorant binding protein lush with propanol 1ooh 
Complex of drosophila odorant binding protein lush with butanol 1ooi 
Crystal structure of lush from drosophila melanogaster at ph 6.5 1org 
The crystal structure of a pheromone binding protein from the cockroach leucophaea maderae reveals a new mechanism of pheromone binding 1ow4 
Crystal structure of a pheromone binding protein from the cockroach leucophaea maderae in complex with the fluorescent reporter ans (1-anilinonaphtalene-8-sulfonic acid), 1p28 
The crystal structure of a pheromone binding protein from the cockroach leucophaea maderae in complex with a component of the pheromonal blend: 3-hydroxy-butan-2-one. 1qwv 
Solution structure of antheraea polyphemus pheromone binding protein (apolpbp) 1t14 
Crystal structure of lush from drosophila melanogaster: apo protein 1tuj 
Solution structure of the honey bee general odorant binding protein asp2 in complex with trimethylsilyl-d4 propionate 1two 
Nmr structure of the pheromone binding protein from antheraea polyphemus at acidic ph 1xfr 
Solution structure of the bombyx mori pheromone-binding protein fragment bmpbp(1-128) at ph 6.5 2erb 
Agamobp1, and odorant binding protein from anopheles gambiae complexed with peg 2fjy 
Crystal structure of b-form bombyx mori pheromone binding protein 2gte 
Drosophila obp lush bound to attractant pheromone 11-cis- vaccenyl acetate 2h8v 
Structure of empty pheromone binding protein asp1 from the honeybee apis mellifera l 2jpo 
Nmr structure of antheraea polyphemus pheromone-binding protein 1 at ph 4.5 2p70 
Bombyx mori pheromone binding protein bound to bell pepper odorant 2p71 
Bombyx mori pheromone binding protein bound to iodohexadecane 2pql 
Crystal structure of anopheles gambiae d7r4-tryptamine complex 2qdi 
Drosophila obp lush d118a mutation 2qeb 
Crystal structure of anopheles gambiae d7r4-histamine complex 2qeh 
Crystal structure of anopheles gambiae d7r4-serotonin complex 2qeo 
Crystal structure of anopheles gambiae d7r4-norepinephrine complex 2qev 
Crystal structure of anopheles gambiae d7r4 2wc5 
Structure of bmori gobp2 (general odorant binding protein 2) 2wc6 
Structure of bmori gobp2 (general odorant binding protein 2) with bombykol and water to arg 110 2wch 
Structure of bmori gobp2 (general odorant binding protein 2) with bombykal 2wcj 
Structure of bmori gobp2 (general odorant binding protein 2) with (10e,12z)-tetradecadien-1-ol 2wck 
Structure of bmori gobp2 (general odorant binding protein 2) without ligand 2wcl 
Structure of bmori gobp2 (general odorant binding protein 2) with (8e,10z)-hexadecadien-1-ol 2wcm 
Structure of bmori gobp2 (general odorant binding protein 2) with (10e)-hexadecen-12-yn-1-ol 3b6x 
Complex of s52a substituted drosophila lush protein with butanol 3b7a 
Complex of s52a substituted droposphila lush protein with ethanol 3b86 
Crystal structure of t57s substituted lush protein complexed with ethanol 3b87 
Complex of t57a substituted droposphila lush protein with butanol 3b88 
Complex of t57a substituted drosophila lush protein with ethanol 3bfa 
Crystal structure of a pheromone binding protein from apis mellifera in complex with the queen mandibular pheromone 3bfb 
Crystal structure of a pheromone binding protein from apis mellifera in complex with the 9-keto-2(e)-decenoic acid 3bfh 
Crystal structure of a pheromone binding protein from apis mellifera in complex with hexadecanoic acid 3bjh 
Soft-sad crystal structure of a pheromone binding protein from the honeybee apis mellifera l. 3cab 
Crystal structure of a pheromone binding protein from apis mellifera soaked at ph 7.0 3cdn 
Crystal structure of a pheromone binding protein from apis mellifera soaked at ph 4.0 3cyz 
Dimeric crystal structure of a pheromone binding protein from apis mellifera in complex with 9-keto-2(e)-decenoic acid at ph 7.0 3cz0 
Dimeric crystal structure of a pheromone binding protein from apis mellifera in complex with the n-butyl benzene sulfonamide at ph 7.0 3cz1 
Dimeric crystal structure of a pheromone binding protein from apis mellifera in complex with the n-butyl benzene sulfonamide at ph 7.0 3cz2 
Dimeric crystal structure of a pheromone binding protein from apis mellifera at ph 7.0 3d73 
Crystal structure of a pheromone binding protein mutant d35a, from apis mellifera, at ph 7.0 3d74 
Crystal structure of a pheromone binding protein mutant d35a, from apis mellifera, soaked at ph 5.5 3d75 
Crystal structure of a pheromone binding protein mutant d35n, from apis mellifera, at ph 5.5 3d76 
Crystal structure of a pheromone binding protein mutant d35n, from apis mellifera, soaked at ph 7.0 3d77 
Crystal structure of a pheromone binding protein mutant d35n, from apis mellifera, soaked at ph 4.0 3d78 
Dimeric crystal structure of a pheromone binding protein mutant d35n, from apis mellifera, at ph 7.0 3dxl 
Crystal structure of aed7 from aedes aegypti 3dy9 
Crystal structure of aed7 potassium bromide soak 3dye 
Crystal structure of aed7-norepineprhine complex 3dzt 
Aed7-leukotriene e4 complex - Links (links to other resources describing this domain)
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INTERPRO IPR006625