This presumed domain is found in the RTC4 protein from yeasts. In Saccharomyces cerevisiae, Cdc13 binds telomeric DNA to recruit telomerase and to "cap" chromosome ends. RTC4 was identified in a screen to identify novel proteins and pathways that cap telomeres, or that respond to uncapped telomeres PMID:18845848. This domain is also found in proteins that contain a DNA-binding myb domain.
This entry represents the C-terminal domain of the RTC4 (restriction of telomere capping protein 4) protein from yeasts. In Saccharomyces cerevisiae, deletion of RTC4 affects the cell response to telomere uncapping [ (PUBMED:18845848) ]. This domain is also found in proteins that contain a DNA-binding myb domain.
Family alignment:
There are 350 RTC4 domains in 350 proteins in SMART's nrdb database.
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Evolution (species in which this domain is found)
Taxonomic distribution of proteins containing RTC4 domain.
This tree includes only several representative species. The complete taxonomic breakdown of all proteins with RTC4 domain is also avaliable.
Click on the protein counts, or double click on taxonomic names to display all proteins containing RTC4 domain in the selected taxonomic class.
A genomewide suppressor and enhancer analysis of cdc13-1 reveals varied cellular processes influencing telomere capping in Saccharomyces cerevisiae.
Genetics. 2008; 180: 2251-66
Display abstract
In Saccharomyces cerevisiae, Cdc13 binds telomeric DNA to recruit telomerase and to "cap" chromosome ends. In temperature-sensitive cdc13-1 mutants telomeric DNA is degraded and cell-cycle progression is inhibited. To identify novel proteinsand pathways that cap telomeres, or that respond to uncapped telomeres, wecombined cdc13-1 with the yeast gene deletion collection and used high-throughputspot-test assays to measure growth. We identified 369 gene deletions, in eightdifferent phenotypic classes, that reproducibly demonstrated subtle geneticinteractions with the cdc13-1 mutation. As expected, we identified DNA damagecheckpoint, nonsense-mediated decay and telomerase components in our screen.However, we also identified genes affecting casein kinase II activity, cellpolarity, mRNA degradation, mitochondrial function, phosphate transport, irontransport, protein degradation, and other functions. We also identified a number of genes of previously unknown function that we term RTC, for restriction oftelomere capping, or MTC, for maintenance of telomere capping. It seems likelythat many of the newly identified pathways/processes that affect growth ofbudding yeast cdc13-1 mutants will play evolutionarily conserved roles attelomeres. The high-throughput spot-testing approach that we describe isgenerally applicable and could aid in understanding other aspects of eukaryoticcell biology.
Links (links to other resources describing this domain)
