Letunic et al. (2017) Nucleic Acids Res doi: 10.1093/nar/gkx922
Letunic et al. (2020) Nucleic Acids Res doi: 10.1093/nar/gkaa937

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EAL Putative diguanylate phosphodiesterase

SMART accession number:	SM00052
Description:	Putative diguanylate phosphodiesterase, present in a variety of bacteria.
Interpro abstract (IPR001633):	This domain is found in diverse bacterial signalling proteins. It is called EAL after its conserved residues. The EAL domain has been shown to stimulate degradation of a second messenger, cyclic di-GMP [ (PUBMED:15063857) (PUBMED:15289546) (PUBMED:15306016) ], and is a good candidate for a diguanylate phosphodiesterase function. Together with the GGDEF domain, EAL might be involved in regulating cell surface adhesiveness in bacteria [ (PUBMED:11557134) ]. The domain contains many conserved acidic residues that could participate in metal binding and might form the phosphodiesterase active site. It often but not always occurs along with IPR000014 and IPR000160 domains that are also found in many signalling proteins .
Family alignment:	View or CHROMA formatHMM/Web logoCLUSTALW formatMSF formatFASTA formatPIR format

There are 149189 EAL domains in 149188 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Taxonomic distribution of proteins containing EAL domain.

  This tree includes only several representative species. The complete taxonomic breakdown of all proteins with EAL domain is also avaliable.

  Click on the protein counts, or double click on taxonomic names to display all proteins containing EAL domain in the selected taxonomic class.

• Metabolism (metabolic pathways involving proteins which contain this domain)

• Click the image to view the interactive version of the map in iPath

  % proteins involved KEGG pathway ID Description 22.22 map02020 Two-component system - General 11.11 map00260 Glycine, serine and threonine metabolism 11.11 map02060 Phosphotransferase system (PTS) 11.11 map00564 Glycerophospholipid metabolism 11.11 map00910 Nitrogen metabolism 11.11 map02030 Bacterial chemotaxis - General 11.11 map00530 Aminosugars metabolism 11.11 map00340 Histidine metabolism This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with EAL domain which could be assigned to a KEGG orthologous group, and not all proteins containing EAL domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

• Structure (3D structures containing this domain)

• 3D Structures of EAL domains in PDB

  PDB code	Main view	Title
  2bas		Crystal Structure of the Bacillus subtilis YkuI Protein, with an EAL Domain.
  2r6o		Crystal structure of putative diguanylate cyclase/phosphodiesterase from Thiobacillus denitrificans
  2w27		CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS YKUI PROTEIN, WITH AN EAL DOMAIN, IN COMPLEX WITH SUBSTRATE C-DI-GMP AND CALCIUM
  3gfx		Klebsiella pneumoniae BlrP1 pH 4.5 calcium/cy-diGMP complex
  3gfy		Klebsiella pneumoniae BlrP1 with FMN and cyclic diGMP, no metal ions
  3gfz		Klebsiella pneumoniae BlrP1 pH 6 manganese/cy-diGMP complex
  3gg0		Klebsiella pneumoniae BlrP1 pH 9.0 manganese/cy-diGMP complex
  3gg1		Klebsiella pneumoniae BlrP1 pH 8.0 calcium/cy-diGMP complex
  3hv8		Crystal structure of FimX EAL domain from Pseudomonas aeruginosa bound to c-di-GMP
  3hv9		Crystal structure of FimX EAL domain from Pseudomonas aeruginosa
  3kzp		Crystal structure of putative diguanylate cyclase/phosphodiesterase from Listaria monocytigenes
  3n3t		Crystal structure of putative diguanylate cyclase/phosphodiesterase complex with cyclic di-gmp
  3pfm		Crystal structure of a EAL domain of GGDEF domain protein from Pseudomonas fluorescens Pf
  3pjt		Structure of Pseudomonas fluorescence LapD EAL domain complexed with c-di-GMP, C2221
  3pju		Structure of Pseudomonas fluorescence LapD EAL domain complexed with c-di-GMP, P6522
  3pjw		Structure of Pseudomonas fluorescence LapD GGDEF-EAL dual domain, I23
  3pjx		Structure of Pseudomonas fluorescence LapD GGDEF-EAL dual domain, P32
  3s83		Crystal structure of EAL domain from Caulobacter crescentus CB15
  3sy8		Crystal structure of the response regulator RocR
  3tlq		Crystal structure of EAL-like domain protein YdiV
  3u2e		EAL domain from Caulobacter crescentus in complex with 5'-pGpG and Mg++
  4afy		Crystal structure of the FimX EAL domain in complex with reaction product pGpG
  4ag0		Crystal structure of FimX EAL domain
  4es4		Crystal structure of YdiV and FlhD complex
  4f3h		The structural of FimXEAL-c-di-GMP from Xanthomonas campestris
  4f48		The X-ray structural of FimXEAL-c-di-GMP-PilZ complexes from Xanthomonas campestris
  4foj		1.55 A Crystal Structure of Xanthomonas citri FimX EAL domain in complex with c-diGMP
  4fok		1.8 A Crystal structure of the FimX EAL domain in complex with c-diGMP
  4fou		Structure of the PilZ-FimX(EAL domain)-c-di-GMP complex responsible for the regulation of bacterial Type IV pilus biogenesis
  4hjf		Crystal structure of EAL domain from Caulobacter crescentus in complex with c-di-GMP and Ca
  4hu3		Crystal structure of EAL domain of the E. coli DosP - monomeric form
  4hu4		Crystal structure of EAL domain of the E. coli DosP - dimeric form
  4j40		Crystal structure of the dual-domain GGDEF-EAL module of FimX from Pseudomonas aeruginosa
  4kie		Crystal structure of the EAL domain of c-di-GMP specific phosphodiesterase YahA
  4lj3		Crystal structure of the EAL domain of c-di-GMP specific phosphodiesterase YahA in complex with substrate c-di-GMP and Ca++
  4lyk		Crystal structure of the EAL domain of c-di-GMP specific phosphodiesterase YahA in complex with activating cofactor Mg++
  4q6j		Crystal Structure of EAL domain Protein from Listeria monocytogenes EGD-e
  4rnf		4RNF
  4rnh		4RNH
  4rni		4RNI
  4rnj		4RNJ
  4y8e		4Y8E
  4y9m		4Y9M
  4y9n		4Y9N
  4y9o		4Y9O
  4y9p		4Y9P
  4y9q		4Y9Q

• Links (links to other resources describing this domain)

• INTERPRO	IPR001633

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