Letunic et al. (2017) Nucleic Acids Res doi: 10.1093/nar/gkx922
Letunic et al. (2020) Nucleic Acids Res doi: 10.1093/nar/gkaa937

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MGS MGS-like domain

SMART accession number:	SM00851
Description:	This domain composes the whole protein of methylglyoxal synthetase and the domain is also found in Carbamoyl phosphate synthetase (CPS) where it forms a regulatory domain that binds to the allosteric effector ornithine. This family also includes inosicase. The known structures in this family show a common phosphate binding site (PUBMED:10526357).
Interpro abstract (IPR011607):	Methylglyoxal synthase (MGS, EC 4.2.3.3), which catalyzes the conversion of dihydroxyacetone phosphate (DHAP) to methylglyoxal (MG) and inorganic phosphate, has been found in many organisms, including enteric bacteria, some gram-positive bacteria, a number of archaebacteria, several yeast species and goat liver [ (PUBMED:23592737) (PUBMED:10388730) ]. The main core of the MGS-like domain, a modified 'Rossmann' fold, is characterised by a five stranded parallel beta-sheet flanked on either side by three and five alpha-helices, respectively [ (PUBMED:14756553) (PUBMED:10089390) ]. MGS-like domains share a conserved phosphate binding site [ (PUBMED:10526357) (PUBMED:29063699) ].
Family alignment:	View or CHROMA formatHMM/Web logoCLUSTALW formatMSF formatFASTA formatPIR format

There are 47239 MGS domains in 47236 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Taxonomic distribution of proteins containing MGS domain.

  This tree includes only several representative species. The complete taxonomic breakdown of all proteins with MGS domain is also avaliable.

  Click on the protein counts, or double click on taxonomic names to display all proteins containing MGS domain in the selected taxonomic class.

• Cellular role (predicted cellular role)

• Cellular role: interaction
  

• Metabolism (metabolic pathways involving proteins which contain this domain)

• Click the image to view the interactive version of the map in iPath

  % proteins involved KEGG pathway ID Description 24.08 map00230 Purine metabolism 22.47 map00240 Pyrimidine metabolism 22.24 map00670 One carbon pool by folate 22.13 map00251 Glutamate metabolism 7.07 map00620 Pyruvate metabolism 0.75 map00252 Alanine and aspartate metabolism 0.40 map00910 Nitrogen metabolism 0.40 map00330 Arginine and proline metabolism 0.40 map00220 Urea cycle and metabolism of amino groups 0.06 map00061 Fatty acid biosynthesis This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with MGS domain which could be assigned to a KEGG orthologous group, and not all proteins containing MGS domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

• Structure (3D structures containing this domain)

• 3D Structures of MGS domains in PDB

  PDB code	Main view	Title
  1a9x		CARBAMOYL PHOSPHATE SYNTHETASE: CAUGHT IN THE ACT OF GLUTAMINE HYDROLYSIS
  1b93		METHYLGLYOXAL SYNTHASE FROM ESCHERICHIA COLI
  1bxr		STRUCTURE OF CARBAMOYL PHOSPHATE SYNTHETASE COMPLEXED WITH THE ATP ANALOG AMPPNP
  1c30		CRYSTAL STRUCTURE OF CARBAMOYL PHOSPHATE SYNTHETASE: SMALL SUBUNIT MUTATION C269S
  1c3o		CRYSTAL STRUCTURE OF THE CARBAMOYL PHOSPHATE SYNTHETASE: SMALL SUBUNIT MUTANT C269S WITH BOUND GLUTAMINE
  1ce8		CARBAMOYL PHOSPHATE SYNTHETASE FROM ESCHERICHIS COLI WITH COMPLEXED WITH THE ALLOSTERIC LIGAND IMP
  1cs0		Crystal structure of carbamoyl phosphate synthetase complexed at CYS269 in the small subunit with the tetrahedral mimic l-glutamate gamma-semialdehyde
  1egh		STRUCTURE OF METHYLGLYOXAL SYNTHASE COMPLEXED WITH THE COMPETITIVE INHIBITOR 2-PHOSPHOGLYCOLATE
  1g8m		CRYSTAL STRUCTURE OF AVIAN ATIC, A BIFUNCTIONAL TRANSFORMYLASE AND CYCLOHYDROLASE ENZYME IN PURINE BIOSYNTHESIS AT 1.75 ANG. RESOLUTION
  1ik4		X-ray Structure of Methylglyoxal Synthase from E. coli Complexed with Phosphoglycolohydroxamic Acid
  1jdb		CARBAMOYL PHOSPHATE SYNTHETASE FROM ESCHERICHIA COLI
  1kee		Inactivation of the Amidotransferase Activity of Carbamoyl Phosphate Synthetase by the Antibiotic Acivicin
  1m6v		Crystal Structure of the G359F (small subunit) Point Mutant of Carbamoyl Phosphate Synthetase
  1m9n		CRYSTAL STRUCTURE OF THE HOMODIMERIC BIFUNCTIONAL TRANSFORMYLASE AND CYCLOHYDROLASE ENZYME AVIAN ATIC IN COMPLEX WITH AICAR AND XMP AT 1.93 ANGSTROMS.
  1oz0		CRYSTAL STRUCTURE OF THE HOMODIMERIC BIFUNCTIONAL TRANSFORMYLASE AND CYCLOHYDROLASE ENZYME AVIAN ATIC IN COMPLEX WITH A MULTISUBSTRATE ADDUCT INHIBITOR BETA-DADF.
  1p4r		Crystal Structure of Human ATIC in complex with folate-based inhibitor BW1540U88UD
  1pkx		Crystal Structure of human ATIC in complex with XMP
  1pl0		Crystal structure of human ATIC in complex with folate-based inhibitor, BW2315U89UC
  1s89		H98N Mutant of Methylglyoxal Synthase from E. coli complexed with Phosphoglycolic Acid
  1s8a		H98Q Mutant of Methylglyoxal Synthase from E. coli complexed with Phosphoglycolic Acid
  1t36		Crystal structure of E. coli carbamoyl phosphate synthetase small subunit mutant C248D complexed with uridine 5'-monophosphate
  1thz		Crystal Structure of Avian AICAR Transformylase in Complex with a Novel Inhibitor Identified by Virtual Ligand Screening
  1vmd		Crystal structure of Methylglyoxal synthase (TM1185) from Thermotoga maritima at 2.06 A resolution
  1wo8		Crystal structure of methylglyoxal synthase from Thermus thermophilus HB8
  1zcz		Crystal structure of Phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase (TM1249) from THERMOTOGA MARITIMA at 1.88 A resolution
  2b1g		Crystal structures of transition state analogue inhibitors of inosine monophosphate cyclohydrolase
  2b1i		crystal structures of transition state analogue inhibitors of inosine monophosphate cyclohydrolase
  2iu0		crystal structures of transition state analogue inhibitors of inosine monophosphate cyclohydrolase
  2iu3		crystal structures of transition state analogue inhibitors of inosine monophosphate cyclohydrolase
  2x8w		The Crystal Structure of Methylglyoxal Synthase from Thermus sp. GH5 Bound to Malonate.
  2xw6		The Crystal Structure of Methylglyoxal Synthase from Thermus sp. GH5 Bound to Phosphate Ion.
  2yvq		Crystal structure of MGS domain of carbamoyl-phosphate synthetase from homo sapiens
  3zzm		Crystal structure of Mycobacterium tuberculosis PurH with a novel bound nucleotide CFAIR, at 2.2 A resolution.
  4a1o		Crystal structure of Mycobacterium tuberculosis PurH complexed with AICAR and a novel nucleotide CFAIR, at 2.48 A resolution.
  4ehi		An X-ray Crystal Structure of a putative Bifunctional Phosphoribosylaminoimidazolecarboxamide Formyltransferase/IMP Cyclohydrolase
  5dot		5DOT
  5dou		5DOU
  5h3l		5H3L

• Links (links to other resources describing this domain)

• PFAM	MGS
  INTERPRO	IPR011607

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