Letunic et al. (2017) Nucleic Acids Res doi: 10.1093/nar/gkx922
Letunic et al. (2020) Nucleic Acids Res doi: 10.1093/nar/gkaa937

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Aldolase_II Class II Aldolase and Adducin N-terminal domain

SMART accession number:	SM01007
Description:	This family includes class II aldolases and adducins which have not been ascribed any enzymatic function.
Interpro abstract (IPR001303):	This entry represents the alpha/beta/alpha domain found in class II aldolases and in adducin (usually at the N terminus of adducin). Proteins containing this domain include: rhamnulose-1-phosphate aldolase ( EC 4.1.2.19 ), L-fuculose phosphate aldolase ( EC 4.1.2.17 ) [ (PUBMED:8515438) (PUBMED:8676381) ] that is involved in the third step in fucose metabolism, L-ribulose- 5-phosphate 4-epimerase ( EC 5.1.3.4 ) involved in the third step of L-arabinose catabolism, a probable sugar isomerase SgbE and the metazoan adducins, which have not been ascribed any enzymatic function but which play a role in cell membrane cytoskeleton organisation [ (PUBMED:1840603) ].
Family alignment:	View or CHROMA formatHMM/Web logoCLUSTALW formatMSF formatFASTA formatPIR format

There are 44565 Aldolase_II domains in 44355 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Taxonomic distribution of proteins containing Aldolase_II domain.

  This tree includes only several representative species. The complete taxonomic breakdown of all proteins with Aldolase_II domain is also avaliable.

  Click on the protein counts, or double click on taxonomic names to display all proteins containing Aldolase_II domain in the selected taxonomic class.

• Cellular role (predicted cellular role)

• Cellular role: metabolism
  

• Literature (relevant references for this domain)

• Primary literature is listed below; Automatically-derived, secondary literature is also avaliable.

  • Dreyer MK, Schulz GE
  • Catalytic mechanism of the metal-dependent fuculose aldolase fromEscherichia coli as derived from the structure.
  • J Mol Biol. 1996; 259: 458-66
  • Display abstract

  • The structure of L-fuculose-1-phosphate aldolase in a cubic crystal formhas been determined with and without the inhibitorphosphoglycolohydroxamate at 2.4 and 2.7 angstrom (1 angstrom = 0.1 nm)resolution, respectively. This inhibitor mimics the enediolate transitionstate of the substrate moiety dihydroxyacetone phosphate. The structuresshowed that dihydroxyacetone phosphate ligates the zinc ion of thismetal-dependent class II aldolase with its hydroxyl and keto oxygen atoms,shifting Glu73 away from the zinc coordination sphere to a non-polarenvironment. At this position Glu73 accepts a proton in the initialreaction step, producing the enediolate which is then stabilized by thezinc ion. The other substrate moiety L-lactaldehyde was modeled, becauseno binding structure is yet available.

  • Dreyer MK, Schulz GE
  • The spatial structure of the class II L-fuculose-1-phosphate aldolase fromEscherichia coli.
  • J Mol Biol. 1993; 231: 549-53
  • Display abstract

  • The three-dimensional structure of L-fuculose-1-phosphate aldolase (FucA)from Escherichia coli was determined by X-ray crystallography at aresolution of 2.13 A. The enzyme is a homotetramer with an M(r) of 23,775per subunit. Since its activity depends on the presence of metal ions(Zn2+) the enzyme belongs to the class II aldolases. As expected fromamino acid sequence comparisons, this first structure of a class IIaldolase shows no similarity to the known structures of class I aldolases.It has some unusual features concerning the overall chain fold, thequaternary structure, and the co-ordination of the catalytically activezinc ion. A sequence comparison with the data bank indicated that themiddle domain of the enzyme L-ribulose-5-phosphate-4-epimerase ishomologous to FucA and may contain an active-center metal ion.

• Structure (3D structures containing this domain)

• 3D Structures of Aldolase_II domains in PDB

  PDB code	Main view	Title
  1dzu		L-Fuculose-1-Phosphate Aldolase from Escherichia coli Mutant T26A
  1dzv		L-Fuculose-1-Phosphate Aldolase from Escherichia coli Mutant Y113F/Y209F
  1dzw		L-Fuculose-1-Phosphate Aldolase from Escherichia coli Mutant F131A
  1dzx		L-Fuculose-1-Phosphate Aldolase from Escherichia coli Mutant R212A
  1dzy		L-Fuculose-1-Phosphate Aldolase from Escherichia coli Mutant E214A
  1dzz		L-Fuculose-1-Phosphate Aldolase from Escherichia coli Mutant Y113F
  1e46		L-Fuculose 1-Phosphate Aldolase from Escherichia coli Mutant E73S
  1e47		L-Fuculose 1-Phosphate Aldolase from Escherichia coli Mutant E73Q
  1e48		L-Fuculose 1-Phosphate Aldolase from Escherichia coli Mutant E73Q/Y113F/Y209F
  1e49		L-Fuculose 1-Phosphate Aldolase from Escherichia coli Mutant N29L/S71A
  1e4a		L-Fuculose 1-Phosphate Aldolase from Escherichia coli Mutant Del(27)
  1e4b		L-Fuculose 1-Phosphate Aldolase from Escherichia coli Mutant N29Q
  1e4c		L-Fuculose 1-Phosphate Aldolase from Escherichia coli Mutant S71Q
  1fua		L-FUCULOSE 1-PHOSPHATE ALDOLASE CRYSTAL FORM T
  1gt7		L-rhamnulose-1-phosphate aldolase from Escherichia coli
  1jdi		CRYSTAL STRUCTURE OF L-RIBULOSE-5-PHOSPHATE 4-EPIMERASE
  1k0w		CRYSTAL STRUCTURE OF L-RIBULOSE-5-PHOSPHATE 4-EPIMERASE
  1ojr		L-rhamnulose-1-phosphate aldolase from Escherichia coli (mutant E192A)
  1pvt		Crystal structure of sugar-phosphate aldolase from Thermotoga maritima
  2fk5		Crystal structure of l-fuculose-1-phosphate aldolase from Thermus thermophilus HB8
  2flf		Crystal structure of l-fuculose-1-phosphate aldolase from Thermus Thermophilus HB8
  2fua		L-FUCULOSE 1-PHOSPHATE ALDOLASE CRYSTAL FORM T WITH COBALT
  2irp		Crystal structure of the l-fuculose-1-phosphate aldolase (aq_1979) from aquifex aeolicus VF5
  2opi		Crystal Structure of L-fuculose-1-phosphate aldolase from Bacteroides thetaiotaomicron
  2uyu		L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT A88F- E192A)
  2uyv		L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT Q6Y- E192A)
  2v29		L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT K15W- E192A)
  2v2a		L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT E192A- K248G-R253A-E254A)
  2v2b		L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT E117S- E192A-K248G-R253A-E254A)
  2v9e		L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT E192A- K248W-A273S)
  2v9f		L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT E192A- K248W-A273S)
  2v9g		L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT Q6Y- L84W-E192A)
  2v9i		L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT E192A- K248W-L274stop)
  2v9l		L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT Q6Y- E192A)
  2v9m		L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT A87M- T109F-E192A)
  2v9n		L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT A88F- E192A)
  2v9o		L-RHAMNULOSE-1-PHOSPHATE ALDOLASE FROM ESCHERICHIA COLI (MUTANT A87M- T109F-E192A)
  2z7b		Crystal Structure of Mesorhizobium loti 3-hydroxy-2-methylpyridine-4,5-dicarboxylate decarboxylase
  3fua		L-FUCULOSE-1-PHOSPHATE ALDOLASE CRYSTAL FORM K
  3m4r		Structure of the N-terminal Class II Aldolase domain of a conserved protein from Thermoplasma acidophilum
  3ocr		Crystal structure of aldolase II superfamily protein from Pseudomonas syringae
  4c24		L-fuculose 1-phosphate aldolase
  4c25		L-fuculose 1-phosphate aldolase
  4fua		L-FUCULOSE-1-PHOSPHATE ALDOLASE COMPLEX WITH PGH
  4m6r		Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme
  4xxf		4XXF

• Links (links to other resources describing this domain)

• PFAM	Aldolase_II
  INTERPRO	IPR001303

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