Letunic et al. (2017) Nucleic Acids Res doi: 10.1093/nar/gkx922
Letunic et al. (2020) Nucleic Acids Res doi: 10.1093/nar/gkaa937

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FACT-Spt16_Nlob FACT complex subunit SPT16 N-terminal lobe domain

SMART accession number:	SM01285
Description:	The FACT or facilitator of chromatin transcription complex binds to and alters the properties of nucleosomes. This family represents the N-terminal lobe of the NTD, or N-terminal domain, and acts as a protein-protein interaction domain presumably with partners outside of the FACT complex PMID:18089575. Knockout of the whole NTD domain, 1-450 residues in UniProt:P32558 in yeast serves to tender the cells sensitive to DNA replication stress but is not lethal. The C-terminal half of NTD is structurally similar to aminopeptidases, and the most highly conserved surface residues line a cleft equivalent to the aminopeptidase substrate-binding site, family peptidase_M24, (PFAM:PF00557) PMID:18089575
Interpro abstract (IPR029148):	Spt16 is a component of the FACT (facilitator of chromatin transcription) complex, a general chromatin factor that acts to reorganise nucleosomes [ (PUBMED:12815073) (PUBMED:10413469) ]. In budding yeasts, the N-terminal domain (NTD) of Spt16 includes a smaller N-terminal lobe (residues 1-175) and a larger C-terminal lobe (residues 176-447). This entry represents the N-terminal lobe domain. Its structure is similar to the RuvC/RNase H family. It may act as a protein-protein interaction domain presumably with partners outside of the FACT complex [ (PUBMED:18089575) ]. The C-terminal lobe of NTD is structurally similar to aminopeptidases, and the most highly conserved surface residues line a cleft equivalent to the aminopeptidase substrate-binding site in peptidase family M24 ( IPR000994 ).
Family alignment:	View or CHROMA formatHMM/Web logoCLUSTALW formatMSF formatFASTA formatPIR format

There are 1566 FACT-Spt16_Nlob domains in 1566 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Taxonomic distribution of proteins containing FACT-Spt16_Nlob domain.

  This tree includes only several representative species. The complete taxonomic breakdown of all proteins with FACT-Spt16_Nlob domain is also avaliable.

  Click on the protein counts, or double click on taxonomic names to display all proteins containing FACT-Spt16_Nlob domain in the selected taxonomic class.

• Cellular role (predicted cellular role)

• Cellular role: transcription
  

• Structure (3D structures containing this domain)

• 3D Structures of FACT-Spt16_Nlob domains in PDB

  PDB code	Main view	Title
  3bip		Crystal structure of yeast Spt16 N-terminal Domain
  3biq		Crystal structure of yeast Spt16 N-terminal Domain
  3bit		Crystal structure of yeast Spt16 N-terminal Domain
  3cb5		Crystal Structure of the S. pombe Peptidase Homology Domain of FACT complex subunit Spt16 (form A)
  3cb6		Crystal Structure of the S. pombe Peptidase Homology Domain of FACT complex subunit Spt16 (form B)
  5ce6		5CE6
  5e5b		5E5B

• Links (links to other resources describing this domain)

• INTERPRO	IPR029148
  PFAM	FACT-Spt16_Nlob

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