Letunic et al. (2017) Nucleic Acids Res doi: 10.1093/nar/gkx922
Letunic et al. (2020) Nucleic Acids Res doi: 10.1093/nar/gkaa937

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Glyco_10 Glycosyl hydrolase family 10

SMART accession number:	SM00633
Description:	-
Interpro abstract (IPR001000):	O-Glycosyl hydrolases ( EC 3.2.1. ) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [ (PUBMED:7624375) (PUBMED:8535779) ]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) website. Glycoside hydrolase family 10 comprises enzymes with a number of known activities; xylanase ( EC 3.2.1.8 ); endo-1,3-beta-xylanase ( EC 3.2.1.32 ); cellobiohydrolase ( EC 3.2.1.91 ). These enzymes were formerly known as cellulase family F. The microbial degradation of cellulose and xylans requires several types of enzymes such as endoglucanases ( EC 3.2.1.4 ), cellobiohydrolases ( EC 3.2.1.91 ) (exoglucanases), or xylanases ( EC 3.2.1.8 ) [ (PUBMED:2252383) (PUBMED:1886523) ]. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family F [ (PUBMED:2806912) ] or as the glycosyl hydrolases family 10 [ (PUBMED:1747104) ]. The overall structure of the GH10 domain corresponds to an eightfold alpha/beta-barrel (TIM-barrel) with a typical deep groove in the centre, allowing an 'endo' type of action on the large polysaccharide backbone [ (PUBMED:17642511) ], [ (PUBMED:23508990) ].
GO process:	carbohydrate metabolic process (GO:0005975)
GO function:	hydrolase activity, hydrolyzing O-glycosyl compounds (GO:0004553)
Family alignment:	View or CHROMA formatHMM/Web logoCLUSTALW formatMSF formatFASTA formatPIR format

There are 11067 Glyco_10 domains in 11041 proteins in SMART's nrdb database.

Click on the following links for more information.

• Evolution (species in which this domain is found)

• Taxonomic distribution of proteins containing Glyco_10 domain.

  This tree includes only several representative species. The complete taxonomic breakdown of all proteins with Glyco_10 domain is also avaliable.

  Click on the protein counts, or double click on taxonomic names to display all proteins containing Glyco_10 domain in the selected taxonomic class.

• Metabolism (metabolic pathways involving proteins which contain this domain)

• Click the image to view the interactive version of the map in iPath

  % proteins involved KEGG pathway ID Description 50.00 map00530 Aminosugars metabolism 50.00 map00500 Starch and sucrose metabolism This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with Glyco_10 domain which could be assigned to a KEGG orthologous group, and not all proteins containing Glyco_10 domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.

• Structure (3D structures containing this domain)

• 3D Structures of Glyco_10 domains in PDB

  PDB code	Main view	Title
  1b30		XYLANASE FROM PENICILLIUM SIMPLICISSIMUM, COMPLEX WITH 1,2-(4-DEOXY-BETA-L-THREO-HEX-4-ENOPYRANOSYLURONIC ACID)-BETA-1,4-XYLOTRIOSE)
  1b31		XYLANASE FROM PENICILLIUM SIMPLICISSIMUM, NATIVE WITH PEG200 AS CRYOPROTECTANT
  1b3v		XYLANASE FROM PENICILLIUM SIMPLICISSIMUM, COMPLEX WITH XYLOSE
  1b3w		XYLANASE FROM PENICILLIUM SIMPLICISSIMUM, COMPLEX WITH XYLOBIOSE
  1b3x		XYLANASE FROM PENICILLIUM SIMPLICISSIMUM, COMPLEX WITH XYLOTRIOSE
  1b3y		XYLANASE FROM PENICILLIUM SIMPLICISSIMUM, COMPLEX WITH XYLOTETRAOSE
  1b3z		XYLANASE FROM PENICILLIUM SIMPLICISSIMUM, COMPLEX WITH XYLOPENTAOSE
  1bg4		XYLANASE FROM PENICILLIUM SIMPLICISSIMUM
  1clx		CATALYTIC CORE OF XYLANASE A
  1e0v		Xylanase 10A from Sreptomyces lividans. cellobiosyl-enzyme intermediate at 1.7 A
  1e0w		Xylanase 10A from Sreptomyces lividans. native structure at 1.2 angstrom resolution
  1e0x		Xylanase 10A from Sreptomyces lividans. xylobiosyl-enzyme intermediate at 1.65 A
  1e5n		E246C mutant of P fluorescens subsp. cellulosa xylanase A in complex with xylopentaose
  1exp		BETA-1,4-GLYCANASE CEX-CD
  1fh7		CRYSTAL STRUCTURE OF THE XYLANASE CEX WITH XYLOBIOSE-DERIVED INHIBITOR DEOXYNOJIRIMYCIN
  1fh8		CRYSTAL STRUCTURE OF THE XYLANASE CEX WITH XYLOBIOSE-DERIVED ISOFAGOMINE INHIBITOR
  1fh9		CRYSTAL STRUCTURE OF THE XYLANASE CEX WITH XYLOBIOSE-DERIVED LACTAM OXIME INHIBITOR
  1fhd		CRYSTAL STRUCTURE OF THE XYLANASE CEX WITH XYLOBIOSE-DERIVED IMIDAZOLE INHIBITOR
  1gok		Thermostable xylanase I from Thermoascus aurantiacus- Crystal form II
  1gom		Thermostable xylanase I from Thermoascus aurantiacus- Crystal form I
  1goo		Thermostable xylanase I from Thermoascus aurantiacus - Cryocooled glycerol complex
  1goq		Thermostable xylanase I from Thermoascus aurantiacus - Room temperature xylobiose complex
  1gor		THERMOSTABLE XYLANASE I FROM THERMOASCUS AURANTIACUS - XYLOBIOSE COMPLEX AT 100 K
  1hiz		Xylanase T6 (Xt6) from Bacillus Stearothermophilus
  1i1w		0.89A Ultra high resolution structure of a Thermostable Xylanase from Thermoascus Aurantiacus
  1i1x		1.11 A ATOMIC RESOLUTION STRUCTURE OF A THERMOSTABLE XYLANASE FROM THERMOASCUS AURANTIACUS
  1isv		Crystal structure of xylanase from Streptomyces olivaceoviridis E-86 complexed with xylose
  1isw		Crystal structure of xylanase from Streptomyces olivaceoviridis E-86 complexed with xylobiose
  1isx		Crystal structure of xylanase from Streptomyces olivaceoviridis E-86 complexed with xylotriose
  1isy		Crystal structure of xylanase from Streptomyces olivaceoviridis E-86 complexed with glucose
  1isz		Crystal structure of xylanase from Streptomyces olivaceoviridis E-86 complexed with galactose
  1it0		Crystal structure of xylanase from Streptomyces olivaceoviridis E-86 complexed with lactose
  1j01		Crystal Structure Of The Xylanase Cex With Xylobiose-Derived Inhibitor Isofagomine lactam
  1k6a		Structural studies on the mobility in the active site of the Thermoascus aurantiacus xylanase I
  1n82		The high-resolution crystal structure of IXT6, a thermophilic, intracellular xylanase from G. stearothermophilus
  1nq6		Crystal Structure of the catalytic domain of xylanase A from Streptomyces halstedii JM8
  1od8		Xylanase Xyn10A from Streptomyces lividans in complex with xylobio-isofagomine lactam
  1r85		Crystal structure of the extracellular xylanase from Geobacillus stearothermophilus T-6 (XT6): The WT enzyme (monoclinic form) at 1.45A resolution
  1r86		Crystal structure of the extracellular xylanase from Geobacillus stearothermophilus T-6 (XT6, monoclinic form): The E159A/E265A mutant at 1.8A resolution
  1r87		Crystal structure of the extracellular xylanase from Geobacillus stearothermophilus T-6 (XT6, monoclinic form): The complex of the WT enzyme with xylopentaose at 1.67A resolution
  1ta3		Crystal Structure of xylanase (GH10) in complex with inhibitor (XIP)
  1tux		HIGH RESOLUTION CRYSTAL STRUCTURE OF A THERMOSTABLE XYLANASE FROM THERMOASCUS AURANTIACUS
  1uqy		Xylanase Xyn10B mutant (E262S) from Cellvibrio mixtus in complex with xylopentaose
  1uqz		Xylanase Xyn10B mutant (E262S) from Cellvibrio mixtus in complex with xylopentaose
  1ur1		Xylanase Xyn10B mutant (E262S) from Cellvibrio mixtus in complex with arabinofuranose alpha-1,3 linked to xylobiose
  1ur2		Xylanase Xyn10B mutant (E262S) from Cellvibrio mixtus in complex with arabinofuranose alpha 1,3 linked to xylotriose
  1us2		Xylanase10C (mutant E385A) from Cellvibrio japonicus in complex with xylopentaose
  1us3		Native xylanase10C from Cellvibrio japonicus
  1v0k		Xylanase Xyn10A from Streptomyces lividans in complex with xylobio-deoxynojirimycin at pH 5.8
  1v0l		Xylanase Xyn10A from Streptomyces lividans in complex with xylobio-isofagomine at pH 5.8
  1v0m		Xylanase Xyn10a from Streptomyces lividans in complex with xylobio-deoxynojirimycin at pH 7.5
  1v0n		Xylanase Xyn10a from Streptomyces lividans in complex with xylobio-isofagomine at pH 7.5
  1v6u		Crystal Structure Of Xylanase From Streptomyces Olivaceoviridis E-86 Complexed With 2(2)-alpha-L-arabinofuranosyl-xylobiose
  1v6v		Crystal Structure Of Xylanase From Streptomyces Olivaceoviridis E-86 Complexed With 3(2)-alpha-L-arabinofuranosyl-xylotriose
  1v6w		Crystal Structure Of Xylanase From Streptomyces Olivaceoviridis E-86 Complexed With 2(2)-4-O-methyl-alpha-D-glucuronosyl-xylobiose
  1v6x		Crystal Structure Of Xylanase From Streptomyces Olivaceoviridis E-86 Complexed With 3(3)-4-O-methyl-alpha-D-glucuronosyl-xylotriose
  1v6y		Crystal Structure Of chimeric Xylanase between Streptomyces Olivaceoviridis E-86 FXYN and Cellulomonas fimi Cex
  1vbr		Crystal structure of complex xylanase 10B from Thermotoga maritima with xylobiose
  1vbu		Crystal structure of native xylanase 10B from Thermotoga maritima
  1w2p		The 3-dimensional structure of a xylanase (Xyn10A) from Cellvibrio japonicus
  1w2v		The 3-dimensional structure of a thermostable mutant of a xylanase ( Xyn10A) from Cellvibrio japonicus
  1w32		The 3-dimensional structure of a thermostable mutant of a xylanase ( Xyn10A) from Cellvibrio japonicus
  1w3h		The 3-dimensional structure of a thermostable mutant of a xylanase ( Xyn10A) from Cellvibrio japonicus
  1xas		CRYSTAL STRUCTURE, AT 2.6 ANGSTROMS RESOLUTION, OF THE STREPTOMYCES LIVIDANS XYLANASE A, A MEMBER OF THE F FAMILY OF BETA-1,4-D-GLYCANSES
  1xyf		ENDO-1,4-BETA-XYLANASE FROM STREPTOMYCES OLIVACEOVIRIDIS
  1xys		CATALYTIC CORE OF XYLANASE A E246C MUTANT
  1xyz		A COMMON PROTEIN FOLD AND SIMILAR ACTIVE SITE IN TWO DISTINCT FAMILIES OF BETA-GLYCANASES
  2bnj		The xylanase TA from Thermoascus aurantiacus utilizes arabinose decorations of xylan as significant substrate specificity determinants.
  2cnc		Family 10 xylanase
  2d1z		Crystal structure of catalytic-site mutant xylanase from Streptomyces olivaceoviridis E-86
  2d20		Crystal structure of michaelis complex of catalytic-site mutant xylanase from Streptomyces olivaceoviridis E-86
  2d22		Crystal structure of covalent glycosyl-enzyme intermediate of catalytic-site mutant xylanase from Streptomyces olivaceoviridis E-86
  2d23		Crystal structure of EP complex of catalytic-site mutant xylanase from Streptomyces olivaceoviridis E-86
  2d24		Crystal structure of ES complex of catalytic-site mutant xylanase from Streptomyces olivaceoviridis E-86
  2dep		Crystal Structure of xylanase B from Clostridium stercorarium F9
  2exo		CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THE BETA-1,4-GLYCANASE CEX FROM CELLULOMONAS FIMI
  2f8q		An alkali thermostable F/10 xylanase from alkalophilic Bacillus sp. NG-27
  2fgl		An alkali thermostable F/10 xylanase from alkalophilic Bacillus sp. NG-27
  2g3i		Structure of S.olivaceoviridis xylanase Q88A/R275A mutant
  2g3j		Structure of S.olivaceoviridis xylanase Q88A/R275A mutant
  2g4f		Structure of S.olivaceoviridis xylanase Q88A/R275A mutant
  2his		CELLULOMONAS FIMI XYLANASE/CELLULASE DOUBLE MUTANT E127A/H205N WITH COVALENT CELLOBIOSE
  2q8x		The high-resolution crystal structure of ixt6, a thermophilic, intracellular xylanase from G. stearothermophilus
  2uwf		Crystal structure of family 10 xylanase from Bacillus halodurans
  2w5f		High resolution crystallographic structure of the Clostridium thermocellum N-terminal endo-1,4-beta-D-xylanase 10B (Xyn10B) CBM22-1- GH10 modules complexed with xylohexaose
  2wys		High resolution crystallographic structure of the Clostridium thermocellum N-terminal endo-1,4-beta-D-xylanase 10B (Xyn10B) CBM22-1- GH10 modules complexed with xylohexaose
  2wze		High resolution crystallographic structure of the Clostridium thermocellum N-terminal endo-1,4-beta-D-xylanase 10B (Xyn10B) CBM22-1- GH10 modules complexed with xylohexaose
  2xyl		CELLULOMONAS FIMI XYLANASE/CELLULASE COMPLEXED WITH 2-DEOXY-2-FLUORO-XYLOBIOSE
  3cuf		Cellulomonas fimi Xylanase/Cellulase Cex (Cf Xyn10A) in complex with cellobiose-like isofagomine
  3cug		Cellulomonas fimi Xylanase/Cellulase Cex (Cf Xyn10A) in complex with cellotetraose-like isofagomine
  3cuh		Cellulomonas fimi Xylanase/Cellulase Cex (Cf Xyn10A) in complex with cellotriose-like isofagomine
  3cui		Cellulomonas fimi Xylanase/Cellulase Cex (Cf Xyn10A) in complex with sulfur substituted beta-1,4 xylotetraose
  3cuj		Cellulomonas fimi Xylanase/Cellulase Cex (Cf Xyn10A) in complex with sulfur substituted beta-1,4 xylopentaose.
  3emc		Crystal structure of XynB, an intracellular xylanase from Paenibacillus barcinonensis
  3emq		Crystal structure of xilanase XynB from Paenibacillus barcelonensis complexed with an inhibitor
  3emz		Crystal structure of xylanase XynB from Paenibacillus barcinonensis complexed with a conduramine derivative
  3mmd		Crystal structure of the W241A mutant of xylanase from Geobacillus stearothermophilus T-6 (XT6) complexed with hydrolyzed xylopentaose
  3ms8		Enzyme-Substrate interactions of IXT6, the intracellular xylanase of G. stearothermophilus.
  3msd		Enzyme-Substrate interactions of IXT6, the intracellular xylanase of G. stearothermophilus.
  3msg		Enzyme-Substrate interactions of IXT6, the intracellular xylanase of G. stearothermophilus.
  3mua		Enzyme-Substrate interactions of IXT6, the intracellular xylanase of G. stearothermophilus.
  3mui		Enzyme-Substrate interactions of IXT6, the intracellular xylanase of G. stearothermophilus.
  3niy		Crystal structure of native xylanase 10B from Thermotoga petrophila RKU-1
  3nj3		Crystal structure of xylanase 10B from Thermotoga petrophila RKU-1 in complex with xylobiose
  3nyd		Crystal Structure of Kemp Eliminase HG-2 Complexed with Transition State Analog 5-Nitro Benzotriazole
  3o2l		Crystal Structure of an Inactive Kemp Elimination Design HG-1
  3rdk		Protein crystal structure of xylanase A1 of Paenibacillus sp. JDR-2
  3ro8		Crystal structure of the catalytic domain of XynA1 from Paenibacillus sp. JDR-2
  3u7b		A new crystal structure of a Fusarium oxysporum GH10 xylanase reveals the presence of an extended loop on top of the catalytic cleft
  3w24		The high-resolution crystal structure of TsXylA, intracellular xylanase from Thermoanaerobacterium saccharolyticum JW/SL-YS485
  3w25		The high-resolution crystal structure of TsXylA, intracellular xylanase from /Thermoanaerobacterium saccharolyticum JW/SL-YS485/: the complex of the E146A mutant with xylobiose
  3w26		The high-resolution crystal structure of TsXylA, intracellular xylanase from /Thermoanaerobacterium saccharolyticum JW/SL-YS485/: the complex of the E146A mutant with xylotriose
  3w27		The high-resolution crystal structure of TsXylA, intracellular xylanase from /Thermoanaerobacterium saccharolyticum JW/SL-YS485/: the complex of the E251A mutant with xylobiose
  3w28		The high-resolution crystal structure of TsXylA, intracellular xylanase from /Thermoanaerobacterium saccharolyticum JW/SL-YS485/: the complex of the E251A mutant with xylotriose
  3w29		The high-resolution crystal structure of TsXylA, intracellular xylanase from /Thermoanaerobacterium saccharolyticum JW/SL-YS485/: the complex of the E251A mutant with xylotetraose
  3wub		3WUB
  3wue		3WUE
  3wuf		3WUF
  3wug		3WUG
  4bs0		Crystal Structure of Kemp Eliminase HG3.17 E47N,N300D Complexed with Transition State Analog 6-Nitrobenzotriazole
  4e4p		Second native structure of Xylanase A1 from Paenibacillus sp. JDR-2
  4f8x		Penicillium canescens endo-1,4-beta-xylanase XylE
  4hu8		Crystal Structure of a Bacterial Ig-like Domain Containing GH10 Xylanase from Termite Gut
  4k68		Structure of a novel GH10 endoxylanase retrieved from sugarcane soil metagenome
  4l4o		The crystal structure of CbXyn10B in native form
  4l4p		the mutant(E139A) structure in complex with xylotriose
  4pmd		4PMD
  4pmu		4PMU
  4pmv		4PMV
  4pmx		4PMX
  4pmy		4PMY
  4pmz		4PMZ
  4pn2		4PN2
  4prw		4PRW
  4pud		4PUD
  4pue		4PUE
  4qce		4QCE
  4qcf		4QCF
  4qdm		4QDM
  4w8l		4W8L
  4xuy		4XUY
  4xv0		4XV0
  4xx6		4XX6
  5ay7		5AY7
  5d4y		5D4Y
  5eb8		5EB8
  5eba		5EBA
  5efd		5EFD
  5eff		5EFF

• Links (links to other resources describing this domain)

• INTERPRO	IPR001000
  PFAM	Glyco_hydro_10

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