The RWD domain is a conserved region of about 110 amino acid residues, which has been identified in the mouse GCN2 eIF2alpha kinase and histidyl-tRNA synthetase and in presumed orthologues in other eukaryotic species from yeast to vertebrates. Additionally, it is also found in WD repeat containing proteins, yeast DEAD (DEXD)-like helicases, many RING-finger containing proteins, the UPF0029 uncharacterised protein family and a range of hypothetical proteins. The RWD domain has been named after the better characterised RING finger and WD repeat containing proteins and DEAD-like helicases. It has been proposed that the RWD domain might have a function in protein interaction [ (PUBMED:11779830) ].
The RWD domain is predicted to have an alpha/beta secondary structure and is thought to be related to ubiquitin-conjugating enzymes (UBCc) domain, although the catalytic cysteine critical for ubiquitin-conjugating activity is not conserved in most members of the novel subfamily [ (PUBMED:11779830) ].
Systematic identification of novel protein domain families associated with nuclear functions.
Genome Res. 2002; 12: 47-56
Display abstract
A systematic computational analysis of protein sequences containing known nuclear domains led to the identification of 28 novel domain families. This represents a 26% increase in the starting set of 107 known nuclear domain families used for the analysis. Most of the novel domains are present in all major eukaryotic lineages, but 3 are species specific. For about 500 of the 1200 proteins that contain these new domains, nuclear localization could be inferred, and for 700, additional features could be predicted. For example, we identified a new domain, likely to have a role downstream of the unfolded protein response; a nematode-specific signalling domain; and a widespread domain, likely to be a noncatalytic homolog of ubiquitin-conjugating enzymes.
Metabolism (metabolic pathways involving proteins which contain this domain)
Click the image to view the interactive version of the map in iPath
This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. Percentage points are related to the number of proteins with RWD domain which could be assigned to a KEGG orthologous group, and not all proteins containing RWD domain. Please note that proteins can be included in multiple pathways, ie. the numbers above will not always add up to 100%.
