Resolvase, N terminal domain
SMART accession number:SM00857
Description: The N-terminal domain of the resolvase family contains the active site and the dimer interface. The extended arm at the C-terminus of this domain connects to the C-terminal helix-turn-helix domain of resolvase.
Interpro abstract (IPR006119):

Site-specific recombination plays an important role in DNA rearrangement in prokaryotic organisms. Two types of site-specific recombination are known to occur:

  1. Recombination between inverted repeats resulting in the reversal of a DNA segment.
  2. Recombination between repeat sequences on two DNA molecules resulting in their cointegration, or between repeats on one DNA molecule resulting in the excision of a DNA fragment.

Site-specific recombination is characterised by a strand exchange mechanism that requires no DNA synthesis or high energy cofactor; the phosphodiester bond energy is conserved in a phospho-protein linkage during strand cleavage and re-ligation.

Two unrelated families of recombinases are currently known [ (PUBMED:3011407) ]. The first, called the 'phage integrase' family, groups a number of bacterial phage and yeast plasmid enzymes. The second [ (PUBMED:2896291) ], called the 'resolvase' family, groups enzymes which share the following structural characteristics: an N-terminal catalytic and dimerization domain that contains a conserved serine residue involved in the transient covalent attachment to DNA, and a C-terminal helix-turn-helix DNA-binding domain ( IPR006120 ).

The N-terminal resolvase/invertase-type recombinase catalytic domain has an alpha/beta fold and consists of a five-stranded mixed beta-sheet surrounded by three alpha helices on one side and one helix on the other [ (PUBMED:7628011) ].

GO process:DNA recombination (GO:0006310)
GO function:DNA binding (GO:0003677), recombinase activity (GO:0000150)
Family alignment:
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There are 61235 Resolvase domains in 61219 proteins in SMART's nrdb database.

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